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Paecilomyces hepialid strain capable of realizing high yield of adenosine and mannite type substances and application

A technology of Paecilomyces spp. and mannitol, which is applied in the field of microorganisms, can solve problems such as difficult artificial cultivation, damage to the ecological environment, and scarcity of natural Cordyceps sinensis resources.

Active Publication Date: 2016-07-20
JIANGSU SUZHONG PHARM GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Natural Cordyceps sinensis resources are scarce, the growth environment is special, the growth cycle is long, and over-harvesting has seriously damaged the ecological environment, and artificial cultivation is extremely difficult

Method used

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  • Paecilomyces hepialid strain capable of realizing high yield of adenosine and mannite type substances and application
  • Paecilomyces hepialid strain capable of realizing high yield of adenosine and mannite type substances and application
  • Paecilomyces hepialid strain capable of realizing high yield of adenosine and mannite type substances and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The isolation of embodiment 1 Cordyceps sinensis endophytic fungus strain

[0017] 1. Medium

[0018] PDA medium: 1.0 L of potato extract, 20.0 g of glucose, and 15.0 g of agar.

[0019] Seed medium: glucose 3%, peptone 1%, KH 2 PO 4 0.1%, MgSO 4 0.05%.

[0020] 2. After surface disinfection of the wild Cordyceps sinensis on the Qinghai-Tibet Plateau, cut into 2mm×2mm small pieces, insert them into PDA medium, and cultivate them at 25°C. When aerial hyphae grow around the tissue, pick them from the edge of the colony with an inoculation loop The mycelium was taken and inserted into a new medium, and the strain was isolated and purified by picking the edge mycelium of the strain several times. After preliminary isolation and purification, 29 strains of bacteria were obtained. According to the observation of morphology, 25 strains were preliminarily determined to be fungi, 3 strains were bacteria, and 1 strain was actinomycetes.

Embodiment 2

[0021] Embodiment 2 shaking flask screening and strain identification

[0022] The 11 strains of filamentous fungi isolated were fermented in shake flasks using the fermentation medium, and indicators such as biomass, adenosine content, and mannitol content were detected, and the strains with good growth and high yields of adenosine and mannitol were screened out. Bacterial strain identification.

[0023] 1. Medium

[0024] Fermentation medium: glucose 3%, peptone 1%, soybean cake powder 2%, KH 2 PO 4 0.1%, MgSO 4 0.05%.

[0025] 2. Detection method

[0026] (1) Biomass detection

[0027] The fermentation broth was centrifuged at 6000r / min for 10min, the supernatant was discarded, and the precipitate was dried in an oven at 105°C until constant weight, and the biomass was calculated. The crushed mycelium was used to detect the content of adenosine and mannitol.

[0028] (2) Detection of adenosine content

[0029] With reference to Chinese Pharmacopoeia 2000 edition tw...

Embodiment 3

[0039] Embodiment 3 optimization of Paecilomyces japonica fermentation medium

[0040] The seed medium is used as the basic medium, and the types of carbon sources, nitrogen sources, inorganic salts, and growth factors are screened and the amount of addition is optimized. The culture conditions are as follows: liquid filling volume 100mL / 500mL, inoculum volume 5% (v / v), temperature 25°C, rotation speed 150r / min, and culture for 6 days.

[0041] 1. Carbon source screening

[0042] The effects of glucose, fructose, sucrose, maltose and lactose on the fermented biomass, adenosine and mannitol yields of Paecilomyces chrysalis were investigated respectively, and it was found that 3% glucose had the best effect.

[0043] 2. Nitrogen source screening

[0044] Using 3% glucose as the optimal carbon source, the effects of nitrate, bean cake powder, corn steep liquor, yeast powder, tryptone and beef extract on the fermentation biomass, adenosine and mannitol production of Paecilomyces...

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Abstract

The invention relates to a paecilomyces hepialid strain capable of realizing high yield of adenosine and mannite type substances and application. The invention discloses the paecilomyces hepialid strain which is capable of realizing high yield of adenosine and mannite type substances and is obtained through being separated from Qinghai-Tibet Plateau wild cordyceps sinensis, and the application of the paecilomyces hepialid strain. The strain is collected in CGMCC (China General Microbiological Culture Collection Center); and the collection number is CGMCC No.11803. The strain is obtained through separation and screening. When an optimum fermentation culture medium disclosed by the invention is used for 500L fermentation tank production; the biomass can reach 36.1 g / L; the content of the adenosine is 0.61 percent; the yield of the adenosine is 220.1 mg / L; the content of the mannite type substances is 12.8 percent; the yield of the mannite type substances is 4.6g / L; and the values are higher than highest reported adenosine (110.2 mg / L) and mannite type substance (1.4g / L) yield of the paecilomyces hepialid strain in the prior art.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Paecilomyces thaliana with high yield of adenosine and mannitol and its application. Background technique [0002] Cordyceps sinensis is a high-grade nourishing and precious Chinese herbal medicine. It has an early history of folk application. It was first recorded in "Materia Medica Congxin". The tonic is the same as bird's nest". Cordyceps sinensis is a complex composed of the fruiting body of Cordyceps sinensis fungus and the sclerotia of zombophilus (the corpse of bat moth larvae). Increase the number of immune system cells, promote the production of antibodies, have a good regulatory effect on the endocrine system and nervous system, and have anti-tumor active substances, with high health care and medicinal value. [0003] Natural Cordyceps sinensis resources are scarce, the growth environment is special, the growth cycle is long, and over-ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/40C12P7/18C12R1/79
CPCC12P7/18C12P19/40C12R2001/79C12N1/145
Inventor 陆震鸣许正宏唐海涛史劲松唐仁茂赵辉朱青
Owner JIANGSU SUZHONG PHARM GRP CO LTD
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