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Gentamicin degradation strain and application thereof

A technology of gentamicin and bacterial strains, which is applied in the field of gentamicin-degrading bacterial strains, can solve problems such as the screening of aminoglycoside antibiotic-degrading bacteria that have not yet been discovered, and achieve good adaptability

Active Publication Date: 2016-07-20
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI +1
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  • Application Information

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Problems solved by technology

However, according to literature and patent searches, at present, most of the studies are on antibiotic-degrading bacteria such as tetracyclines, sulfonamides, β-lactams, fluoroquinolones and macrolides, and no screening of aminoglycoside antibiotic-degrading bacteria has been found.

Method used

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  • Gentamicin degradation strain and application thereof
  • Gentamicin degradation strain and application thereof
  • Gentamicin degradation strain and application thereof

Examples

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experiment example

[0048] 1. Determination of domestication and degradation rate of bacterial flora

[0049] (1), source of flora: get 10g of medicinal residues from 4 different sources (4 sources: fermentation tank sludge, anaerobic tank sludge, aerobic tank sludge and workshop waste residue) in the pool containing 90g Add an appropriate amount of glass beads to a 250ml Erlenmeyer flask of bacterial water, shake in a constant temperature shaking incubator for 5-10 minutes to fully disperse the sample, and then take out the dregs leachate for later use.

[0050] (2) Bacterial flora domestication culture: take 5 mL of the above-mentioned dregs leachate and add it to 95 mL of liquid BPM containing a certain amount of gentamicin (using gentamicin standard), and place it at a constant temperature of 30 ° C and 150 r / min. Acclimatized in a shaker.

[0051] The domestication is divided into four cycles. The domestication pressure is to increase the concentration of gentamicin from 50mg / L to 200mg / L s...

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Abstract

The invention discloses Brevendimonas diminuta BZC-3. The Brevendimonas diminuta BZC-3 was collected in the General Microbiological Center of China Microbiological Culture Collection Management Committee on January 15th, 2016, and the collection number is CGMCC (China General Microbiological Culture Collection Center) NO. 12070. The strain can be applied to degradation of gentamicin, and the method for degrading gentamicin refers to inoculating Brevendimonas diminuta BZC-3 to a system containing gentamicin to be cultured to realize the degradation of gentamicin. The Gentamicin degradation strain, namely Brevendimonas diminuta BZC-3 can be used for treating pharmaceutical wastes, including wastewater, waste residues and the like with gentamicin residues to reduce damages of gentamicin on the environment, humans and animals so as to solve the problem of antibiotic pollution.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a bacterial strain for degrading gentamicin and its application. Background technique [0002] Antibiotics are a class of secondary metabolites produced by microorganisms (including bacteria, fungi, and actinomycetes) that have anti-pathogen or other activities and can interfere with the developmental functions of other living cells. As antibacterial or bactericidal drugs, antibiotics have been widely used in the treatment of human diseases, livestock and aquaculture, etc., mainly including tetracyclines, sulfonamides, β-lactams, fluoroquinolones, aminoglycosides and large cyclic lactones, etc. my country is a big country in the production and use of antibiotics, with an annual production of 210,000 tons of antibiotics and a usage of 189,000 tons, of which veterinary antibiotics account for more than half of the usage. Studies have found that after organisms ingest a large amount o...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A62D3/02C12R1/01C02F101/38A62D101/28
CPCA62D3/02A62D2101/28C02F3/34C02F2101/38C12N1/20C12N1/205C12R2001/01
Inventor 李兆君刘元望葛祥斌郝金海胡海燕冯瑶杜德维章程王莉莉马金凤
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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