A Newcastle Disease Virus Piezoelectric Immunosensing Detection Method

Abstract

The invention discloses a Newcastle disease virus piezoelectric immunosense detection method.The Newcastle disease virus piezoelectric immunosense detection metho comprises the steps that 1, the surfaces of quartz crystals are coated with a staphylococcus A protein; 2, then a prepared Newcastle disease virus monoclonal antibody is combined with the staphylococcus A protein to obtain a Newcastle disease virus piezoelectric immunosenser; 3, the Newcastle disease virus piezoelectric immunosenser is inserted into a detected sample, whether the quartz crystals produce resonant frequency change or not is detected through a detector, if the resonant frequency change is produced, it is indicated that a Newcastle disease virus exists in the detected sample, and if not, it is indicated that no Newcastle disease virus exists in the detected sample.The Newcastle disease virus piezoelectric immunosense detection method can perform online NDV detection in real time and has higher sensitivity and specificity, and a result is produced within a linear detection range of 2.56-150 ng / ml within 40 minutes.The Newcastle disease virus piezoelectric immunosense detection method has higher sensitivity and accuracy and is rapid and low in cost.

Description

technical field [0001] The invention relates to virus detection in veterinary bioproducts, immunology and biomedical engineering, in particular to a piezoelectric immunosensing detection method for Newcastle disease virus, which belongs to the field of veterinary biotechnology. Background technique [0002] Newcastle Disease (ND) is an acute, septic, and highly contagious animal disease. It spreads rapidly and has high morbidity and mortality. Once an outbreak occurs, it will cause immeasurable losses to the poultry industry. In the "National Medium and Long-Term Animal Disease Prevention and Control Plan (2012-2020), Newcastle disease is listed as one of the major animal diseases that are prioritized for prevention and control. Therefore, animal epidemic control agencies, animal health supervision agencies and farms attach great importance to the prevention and control of Newcastle disease. [0003] There are many methods for diagnosing Newcastle disease, but all of them h...

AI Technical Summary

Problems solved by technology

Hemagglutination-hemagglutination inhibition test (HA-HI) and enzyme-linked immunosorbent assay (ELISA) take a long time, the specificity is not strong, and the procedure is relatively complicated; chicken embryo virus isolation method is a sensitive and specific method, but the whole process is about It needs about 3 weeks, and the operation is cumbersome, which is not conducive to on-site quarantine or monitoring; CN1827775A (patent application number is 200510008997.6) "nucleoside sequence, kit and inspection method for detecting Newcastle virus", this invention selects Newcastle disease virus F gene (synthetic F protein) as the target region, select a conservative region that can reflect the characteristics of the cleavage site and lack secondary structure, design multiple pairs of primers and probes, and establish a fluorescent PCR to detect Newcastle disease virus. This method is 100-1000 times more sensitive than the traditional PCR method. times, but it takes about 4 hours from sample processing to the result. The implementation of this method still needs expensive instruments such as fluorescent quantitative PCR instrument, so it is not suitable to be promoted at the grassroots level

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