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A rooting method for tissue-cultured shoots of cinnamomum camphora (L.) presl

A technology of sassafras oil and camphor tree, which is applied in the field of plant propagation, can solve the problems of difficult rooting of subculture buds, easy blackening of the root system, and long initial rooting time, so as to improve the rooting rate and transplanting survival rate, and shorten the rooting cycle , the effect of high rooting rate

Active Publication Date: 2016-08-10
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a fast rooting method for the problems of the existing material oil dual-purpose sassafras oil type camphor tree tissue secondary bud rooting difficulty, low rooting rate, long initial rooting time, few hair roots, and easy blackening of the root system. , the rooting method of safrole type camphor tree tissue culture buds with good effect

Method used

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  • A rooting method for tissue-cultured shoots of cinnamomum camphora (L.) presl
  • A rooting method for tissue-cultured shoots of cinnamomum camphora (L.) presl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Select the safrole-type camphor tree secondary buds that have been cultured for 35 days in conventional tissue culture. After disinfecting the surface of the bottle, in the sterile space on the ultra-clean workbench, select the secondary buds that grow robustly and have a height of ≥ 2cm. Cut the single bud at 1-2mm below the node, and remove the base leaves and petioles. Then insert the 0.4-0.5cm of the base of the lower end of the bud stem of the single bud into ascorbic acid (VC) 50mg / L sterile solution and soak for 1.5h to perform rooting pretreatment.

[0025] Insert pretreated single shoots vertically into rooting medium. The formula of described rooting medium is: improved ER+NAA 2-3mg / L+ABT 1 0.5-1mg / L + ascorbic acid (VC) 30mg / L + sucrose 15g / L + agar powder 5.8g / L. Among them, the formulation of the improved ER medium is: NH 4 NO 3 1200mg / L + KNO 3 1900mg / L + CaCl 2 .2H 2 O440mg / L + MgSO 4. .7H 2 O 370mg / L + KH 2 PO 4 340mg / L + H 3 BO 3 0.63m...

Embodiment 2

[0029] Select the secondary buds of safrole-type camphor tree that have been cultured for 36 days in the conventional tissue culture. After the surface of the bottle is disinfected, in the sterile space on the ultra-clean workbench, select the secondary buds that grow robustly and have a height of ≥ 2cm. Cut the single bud at 1-2mm below the node, and remove the base leaves and petioles. Then insert the 0.4-0.5cm of the base of the lower end of the bud stem of the single bud into ascorbic acid (VC) 50mg / L sterile solution and soak for 1.5h to perform rooting pretreatment.

[0030] Insert pretreated single shoots vertically into rooting medium. The formula of described rooting medium is: improved ER+NAA 2-3mg / L+ABT 1 0.5-1mg / L + ascorbic acid (VC) 30mg / L + sucrose 15g / L + agar powder 5.8g / L. Among them, the formulation of the improved ER medium is: NH 4 NO 3 1200mg / L + KNO 3 1900mg / L + CaCl 2 .2H 2 O440mg / L + MgSO 4. .7H 2 O 370mg / L + KH 2 PO 4 340mg / L + H 3 BO ...

Embodiment 3

[0034] Select the secondary buds of sassafras type camphor tree that have been cultured for 38 days in the strong buds of conventional tissue culture. After disinfecting the surface of the bottle, in the sterile space on the ultra-clean workbench, select the secondary buds that grow robustly and have a height of ≥ 2cm. Cut the single bud at 1-2mm below the node, and remove the base leaves and petioles. Then insert the 0.4-0.5 cm of the base of the lower end of the single bud stem into ascorbic acid (VC) 50 mg / L sterile solution and soak for 2 hours to perform rooting pretreatment.

[0035] Insert pretreated single shoots vertically into rooting medium. The formula of described rooting medium is: improved ER+NAA 2-3mg / L+ABT 1 0.5-1mg / L + ascorbic acid (VC) 30mg / L + sucrose 15g / L + agar powder 5.8g / L. Among them, the formulation of the improved ER medium is: NH 4 NO 3 1200mg / L + KNO 3 1900mg / L + CaCl 2 .2H 2 O440mg / L + MgSO 4. .7H 2 O 370mg / L + KH 2 PO 4 340mg / L +...

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Abstract

A rooting method for tissue-cultured shoots of cinnamomum camphora (L.) presl is disclosed. The method includes selecting cinnamomum camphora (L.) presl subcultured shoots after 35-40 d strong-shoot culture in conventional tissue culture, cutting single shoots robust in growth and having height not less than 2 cm from the subcultured shoots, inserting into an ascorbic acid sterile solution, soaking for pretreatment, vertically inserting the pretreated single shoots into a rooting culture medium, performing rooting culture in a specific light-temperature environment, conveying to a seedling growing house under natural environment conditions, and performing seedling hardening to obtain seedlings of the cinnamomum camphora (L.) presl. Through pretreating the subcultured shoots, the sassafras oil content in a notch is reduced, the rooting period is shortened, the rooting percentage is high, quality is good, and the survival rate of transplanting of the tissue-cultured rooted seedlings is high. Industrialized tissue-cultured seedling breeding can be achieved. High-quality seedlings can be provided for construction of a natural perfume artificial clonal plantation. The method has good economic benefit, ecological benefit and social benefit.

Description

technical field [0001] The invention belongs to the technical field of plant propagation and relates to the tissue culture technology of camphor tree, in particular to a rooting method of sasrole-type camphor tree tissue culture buds. Background technique [0002] Sassafras camphor tree is a kind of chemical camphor tree (Cinnamomum camphora (L.) Presl), also known as camphor, which is a national second-class protected plant, mainly distributed in Jiangxi, Hunan, Guangdong, Fujian, Hainan, Guangxi, Sichuan, Taiwan and other places, Vietnam, South Korea and Japan are also distributed. Grows in hills and low mountains. It is a multi-purpose excellent tree species that is used for wood collection, oil, flavor and fragrance, medicine, and landscape gardening. Sasrole has the unique aroma of trees and is a very useful natural fragrance. ) and other products, it is also an important pharmaceutical intermediate, which is widely used in industrial production such as light chemica...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 蔡玲韦颖文黄艳陆顺忠黄金使黄宏喜梁晓静丘米杨素华覃玉凤覃杰
Owner GUANGXI FORESTRY RES INST
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