Method for producing combined inactivate vaccine of escherichia coli disease and pasteurellosis in yak

A technology of Escherichia coli and double inactivated vaccine, which is applied in the fields of biology and veterinary biological products, can solve problems such as double vaccine without yak infectious disease, and achieves reduction of immunization workload and simple production process. , easy to use effect

Inactive Publication Date: 2016-08-10
青海生物药品厂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And in the prior art, there is no dual vaccine about the prevention and treatment of yak infectious diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of bacterial solution for seedling preparation

[0024] (1) Take one vacuum freeze-dried strain of Escherichia coli (Tibetan-9903) preserved at 0°C, thaw the freeze-dried strain and culture it in a liquid medium, and streak two pairs of ordinary agar for each liquid culture Plate, select 5 round, smooth, edge-tidy, and moderately sized colonies, culture them at 37°C for 20 hours, inoculate them into 2.5% peptone yak broth medium, place them at 37°C for 24 hours with 160r / min shaking After the inspection and pure inspection, inoculate the culture medium of 2.5% peptone yak broth in a large Erlenmeyer flask, and place it at 37°C for 24 hours with shaking at 160r / min.

[0025] (2) Resuscitate the Pasteurella multocida strains and culture and proliferate them. After submerged aerated fermentation culture or flat bottle solid culture, the bacterial liquid is collected and concentrated for later use.

[0026] 2. culture medium adopts the self-made culture medi...

Embodiment 2

[0043] safety test

[0044] Twenty healthy Tibetan yak calves aged 1-3 months were selected and randomly divided into 5 groups, 4 in each group. Groups 1-4 were the experimental groups, and each group was inoculated with a batch of yak Escherichia coli and pasteurellosis inactivated vaccines, each head was inoculated with 2 mL; yaks in groups 1 and 3 were inoculated subcutaneously at the neck; yaks in groups 2 and 4 were inoculated subcutaneously. Inoculate the buttock muscle. Fourteen days after the yaks were inoculated, 4 mL of the vaccine was repeated at the same site as the first inoculation. Group 5 was the control group, and the dose and method of injecting broth to each yak were the same as those in the experimental group. The results were observed up to 14 days after the initial inoculation and re-inoculation in the test group and the control group.

[0045] The yaks in the test group were observed to be healthy and alive after the initial vaccination and after the ...

Embodiment 3

[0047] immune potency test

[0048] Twenty healthy Tibetan yak calves aged 1-3 months were selected and randomly divided into 5 groups, 4 in each group. Groups 1-4 were the experimental groups, and each group was inoculated with a batch of yak Escherichia coli and pasteurellosis inactivated vaccines, each head was inoculated with 2 mL; yaks in groups 1 and 3 were inoculated subcutaneously at the neck; yaks in groups 2 and 4 were inoculated subcutaneously. Inoculate the buttock muscle. On the 14th day after the yak was inoculated, 2 mL of the vaccine was repeated at the same site as the first inoculation. Group 5 was the control group, and the dose and method of injecting broth to each yak were the same as those in the experimental group. 24 hours after re-inoculation, 1-2 groups of yaks were injected intramuscularly with 2M LD (2 times the minimum lethal dose) of Escherichia coli in yaks respectively, and observed for 14 days. Groups 3-5 of yaks were injected intramuscularl...

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PUM

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Abstract

The invention relates to a method for producing a combined inactivate vaccine of escherichia coli disease and pasteurellosis in yak. The method disclosed by the invention, which makes use of a self-prepared culture medium, is simple in production process and low in cost; safety tests and immunity tests on the yak prove that the combined inactivate vaccine disclosed by the invention has an immunizing effect on the escherichia coli disease and pasteurellosis; the combined inactivate vaccine is more convenient to use than a single vaccine, and the combined inactivate vaccine is capable of preventing the two diseases just by immunizing once, so that the workload of immunization is reduced, and immunity paralysis and immunity failure caused by frequent immunization are avoided; and the combined inactivate vaccine has the advantages of being good in safety, high in immunizing efficacy, long in shelf life and the like.

Description

[0001] Technical field The present invention relates to a method for producing Escherichia coli and Pasteurella dual inactivated vaccine, which belongs to the field of biology, especially the field of veterinary biological products. Background technique [0002] Yaks are an important livestock that Tibetan herdsmen depend on for their survival. There are about 50 million yaks in Tibet. Yak Escherichia coli disease is seriously endangering the yak breeding industry. Currently, the incidence rate of Escherichia coli disease in yaks is 10%, and the mortality rate is 25%. The death of yaks has brought great economic losses to farmers and herdsmen. A few days ago, the only research on yak Escherichia coli disease vaccine was inactivated vaccine of yak Escherichia coli disease with aluminum glue adjuvant. The disease first occurred in female yaks, and lactating female yaks were the most susceptible, followed by 1-4 month-old calves, followed by male yaks. Sick and infected yaks a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/108A61K39/102A61P31/04C12N1/20C12R1/19
CPCA61K39/0258A61K39/102A61K2039/521A61K2039/552A61K2039/70C12N1/20Y02A50/30
Inventor 申登庆王文卓张林青李晓东文进才让
Owner 青海生物药品厂有限公司
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