Method for producing combined inactivate vaccine of escherichia coli disease and pasteurellosis in yak

Abstract

The invention relates to a method for producing a combined inactivate vaccine of escherichia coli disease and pasteurellosis in yak. The method disclosed by the invention, which makes use of a self-prepared culture medium, is simple in production process and low in cost; safety tests and immunity tests on the yak prove that the combined inactivate vaccine disclosed by the invention has an immunizing effect on the escherichia coli disease and pasteurellosis; the combined inactivate vaccine is more convenient to use than a single vaccine, and the combined inactivate vaccine is capable of preventing the two diseases just by immunizing once, so that the workload of immunization is reduced, and immunity paralysis and immunity failure caused by frequent immunization are avoided; and the combined inactivate vaccine has the advantages of being good in safety, high in immunizing efficacy, long in shelf life and the like.

Description

[0001] Technical field The present invention relates to a method for producing Escherichia coli and Pasteurella dual inactivated vaccine, which belongs to the field of biology, especially the field of veterinary biological products. Background technique

[0002] Yaks are an important livestock that Tibetan herdsmen depend on for their survival. There are about 50 million yaks in Tibet. Yak Escherichia coli disease is seriously endangering the yak breeding industry. Currently, the incidence rate of Escherichia coli disease in yaks is 10%, and the mortality rate is 25%. The death of yaks has brought great economic losses to farmers and herdsmen. A few days ago, the only research on yak Escherichia coli disease vaccine was inactivated vaccine of yak Escherichia coli disease with aluminum glue adjuvant. The disease first occurred in female yaks, and lactating female yaks were the most susceptible, followed by 1-4 month-old calves, followed by male yaks. Sick and infected yaks a...