Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel sweetening agent and application thereof

A technology for daily use and condiments, which is applied in the fields of application, antiviral agents, and cosmetic preparations, etc. It can solve the problems of reduced sweetness, non-specific positions of enzyme-transferred glycosyl groups, and impure products, etc., and achieves low production costs. , high purity, easy purification effect

Inactive Publication Date: 2016-08-10
NANJING NUOYUN BIOLOGICAL TECH CO LTD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that stevia can be modified by cyclodextrin glycosyltransferase to improve its taste and taste, but the position of the enzyme to transfer glycosyl groups is not specific, the product is not pure, and its sweetness is also seriously reduced

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel sweetening agent and application thereof
  • Novel sweetening agent and application thereof
  • Novel sweetening agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Construction and induced expression of recombinant Escherichia coli

[0021] The recombinant Escherichia coli expression strain containing the target gene is obtained by using molecular biology and genetic engineering techniques, and then the recombinant Escherichia coli is fermented and cultured to induce expression to prepare recombinant cells containing the target protein. The specific steps are as follows:

[0022] 1) Synthesize the required primer fragments, obtain the required UDP-glucosyltransferase M303 encoding DNA fragments by PCR amplification, and integrate them into the expression cassette of the pNYK expression vector through homologous recombination technology.

[0023] 2) Transform the recombinant plasmid into Escherichia coli to obtain the engineering bacterium J303 containing the target gene.

[0024] 3) Put 1ml of engineered bacteria J303 in TB medium, shake culture at 250rpm, 37°C until OD600=1.0, add final concentration of 0.1mM IPTG, shake culture...

Embodiment 2

[0026] Preparation of Compound (Ⅲ) Using Compound (I) as Substrate

[0027]

[0028] Take 330 mg of wet bacteria M30, resuspend the cells with sterile water, and ultrasonically break the cells in an ice bath, which is the crude enzyme solution used in the reaction. Accurately weigh the sample and prepare a 1.85ml system, wherein the final concentration of the substrate compound (I) is 2.0g / L, UDP-glucose is 2.70g / L, and 0.286g / L of MgCl is added 2 ; Then add crude enzyme solution, and add Tris-HCl buffer solution (pH8.0) to the system of 1.85ml, start the reaction. Shake at 150 rpm for 30 hours at 25°C, and boil at 100°C to terminate the reaction. Centrifuge at 13000 g for 10 min, take the supernatant as a sample, use macroporous resin to perform initial purification on the sample, and use LC-MS method to detect the purity of the purified compound (Ⅲ).

Embodiment 3

[0030] Preparation of Compound (Ⅴ) Using Compound (Ⅲ) as Substrate

[0031]

[0032] Take 20mg of compound (I), add 2ml of KOH with a concentration of 0.1g / ml, 2ml of methanol in sequence, the reflux time is 90min, and the temperature of the water bath is 85°C. Finally, it was cooled to room temperature, and concentrated hydrochloric acid was added to adjust the pH to 7.0 to terminate the reaction. The reaction samples were subjected to HPLC and MS for detection.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a novel sweetening agent and application thereof, and belongs to the technical field of food chemical industry. The method relates to a method for production of a novel stevioside derivative compound (V) by alkali hydrolysis from a raw material of stevioside derivative compound (III). The preparation method of novel stevioside compound (V)by alkaline hydrolysis of stevioside derivative compound (III) is simple for operation, low in cost, and easy to control; and purification process is easy and reaches high purity, can be used for food and beverage industries, and has great potential application value.

Description

technical field [0001] The invention relates to a novel sweetener and its application, belonging to the technical field of food chemical industry. Background technique [0002] Stevioside is a new type of natural sweetener extracted from the leaves and stems of the Compositae herb Stevia rebaudiana. It is a natural, green and health-care functional food. It has the characteristics of high and low calorie. Its sweetness is 200-300 times that of sucrose, and its calorific value is only 1 / 300 of that of sucrose. It is an ideal sweetener that can replace sucrose. As human beings pay more attention to health and green, stevioside is called by food scientists as the most promising sweetener in the future world. [0003] Stevioside is considered by the international medical community as a good nutritional supplement and health medicine for human beings. A large number of scientific experiments have proved that stevioside is beneficial to the regulation of blood sugar and blood pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/00C12P19/56C12P19/18A61K31/704A61P3/10A61P3/04A61P9/10A61P17/02A61P9/12A61P9/00A61P31/04A61P11/00A61P31/16A61P1/02A61K8/60A23L33/10A23L33/125A23L2/60A23L2/52A61Q19/00A61Q19/08
CPCC07H15/256A23L2/52A23L2/60A23V2002/00A61K8/602A61Q19/00A61Q19/08C07H1/00C12P19/18C12P19/56A23V2200/312A23V2200/326A23V2200/328A23V2200/332A23V2250/60
Inventor 朱惠霖丁雪峰刘怡冰
Owner NANJING NUOYUN BIOLOGICAL TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com