Method for preparing yellow pigment from phlorizin through two-enzyme process conversion

A technology of phlorizin and yellow pigment, which is applied in the field of enzymatic synthesis of food coloring, which can solve the problems of long time required for yellow pigment and slow enzymatic oxidation reaction, and achieve obvious social and economic benefits, speed and efficiency improvement , the effect of high purity

Inactive Publication Date: 2016-08-10
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because phlorizin is relatively stable, its enzymatic oxidation reaction is slow, and it takes a long time to prepare yellow pigment with it as a substrate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for preparing yellow pigment by converting phloridzin by a double-enzyme method, comprising the following steps:

[0016] 1) Dissolve 5g of phlorizin in 5L of 0.01M citric acid-disodium hydrogen phosphate buffer solution with a pH of 6.5, add 25000U of β-glucosidase and 100000U of mushroom polyphenol oxidase at the same time, feed in oxygen and stir to make The dissolved oxygen saturation in the reaction solution reaches about 30%, and the enzymatic conversion reaction is carried out at 30° C., and after 4 hours, it is heated to 80° C. to inactivate the enzyme for 5 minutes to obtain the conversion solution.

[0017] 2) Add 1:1 ethyl acetate to the conversion solution obtained in step 1) for extraction, repeat the operation 4 times to remove unreacted phlorizin, and concentrate the lower aqueous phase at 50-55°C under reduced pressure and then freeze Dry to obtain crude yellow pigment.

[0018] 3) The crude yellow pigment obtained in step 2) was dissolved in m...

Embodiment 2

[0020] A method for preparing yellow pigment by converting phloridzin by a double-enzyme method, comprising the following steps:

[0021] 1) Dissolve 3g of phlorizin in 3L of 0.01M citric acid-disodium hydrogen phosphate buffer solution with a pH of 6.0, add 10000U β-glucosidase and 30000U mushroom polyphenol oxidase at the same time, feed in oxygen and stir to make The dissolved oxygen saturation in the reaction solution reaches about 35%, and the enzymatic conversion reaction is carried out at 28° C., and after 8 hours, it is heated to 80° C. to inactivate the enzyme for 5 minutes to obtain the conversion solution.

[0022] 2) Add 1:1 ethyl acetate to the conversion solution obtained in step 1) for extraction, repeat the operation 4 times to remove unreacted phlorizin, and concentrate the lower aqueous phase at 45-50°C under reduced pressure and then freeze Dry to obtain crude yellow pigment.

[0023] 3) The crude yellow pigment obtained in step 2) was dissolved in methanol...

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PUM

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Abstract

The invention discloses a method for preparing yellow pigment from phlorizin through two-enzyme process conversion. The method is applicable to preparation of water soluble yellow pigment while phlorizin is taken as a raw material. The method disclosed by the invention comprises the following steps: adding beta-glucosaccharase and polyphenol oxidase into a phlorizin enzymatic oxidation reaction system at the same time, so that part of phlorizin is decomposed into phloretin by the beta-glucosaccharase in advance, and reaction activity is improved; then carrying out enzymatic oxidation under the action of polyphenol oxidase, so that reaction rate and efficiency are greatly improved; and further extracting and purifying, so that the water soluble yellow pigment is obtained. The yellow pigment is bright yellow under the condition that pH is 2-5 and has biological activities such as oxidation resistance. By adopting the method disclosed by the invention for preparing apple yellow pigment, enzymatic conversion rate of phlorizin is effectively improved, and the problem that cost is too high as phloretin is directly adopted as an oxidization substrate raw material is avoided, so that the method disclosed by the invention has a relatively good application prospect.

Description

technical field [0001] The invention relates to the technical field of enzymatic synthesis of food pigments, in particular to a method for preparing yellow pigment by converting phlorizin with a double enzyme method. Background technique [0002] With the progress of society and the improvement of people's living standards, more and more attention has been paid to the impact and harm of food synthetic coloring on human health, and the demand for natural food coloring is increasing. Yellow pigment is a kind of pigment that is very much needed in the food and cosmetic industry. In the past, it was usually replaced by artificial tartrazine. However, this synthetic pigment is easy to cause asthma and rubella, so it has been banned in many countries. However, it has always been a difficult problem to find natural water-soluble yellow pigments, and the few natural yellow pigments are greatly limited in application due to various defects. For example, the water solubility of curcu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/26
CPCC12P7/26
Inventor 焦中高刘杰超张春岭王雪晖陈大磊杨文博
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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