Detection method of ginsenoside in evodia decoction and application thereof

A detection method, ginsenoside technology, applied in Evodia decoction, the detection field of ginsenoside in vivo and in vitro, can solve the problems of poor UV absorption, low bioavailability, and poor absorption of saponins, and achieve simple and fast operation, Accurate and reproducible results

Inactive Publication Date: 2016-08-10
SHENYANG PHARMA UNIVERSITY
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Saponins have poor absorption and low bioavailability, coupled with their poor UV absorption, it is difficult for ordinary detectors to reach their detection limits

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of ginsenoside in evodia decoction and application thereof
  • Detection method of ginsenoside in evodia decoction and application thereof
  • Detection method of ginsenoside in evodia decoction and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: chromatographic conditions: chromatographic column is Agilent ZORBAX SB-C18 column (4.6mm * 150mm, 5 μ m), mobile phase is the formic acid solution of methanol-0.1%, gradient elution (4min-58% methanol, 6min-90% methanol, 6.01min-58% methanol). The flow rate was 0.5mL·min-1, and the column temperature was 20°C.

[0020] Mass spectrometry conditions: ion injection voltage 5500V, source gas 1 at 310.275KPa, source gas 2 at 344.75KPa, curtain gas at 137.9KPa, source temperature at 500°C. The detection method is positive ion detection, the scanning method is selective reaction monitoring (MRM) method, and the ion pair (m / z) used for quantitative analysis: 823.6 / 203.1 (ginsenoside Rg 1 ), 969.7 / 789.0 (ginsenoside R e ), 1131.8 / 365.1 (ginsenoside Rb 1 ) and 823.6 / 789.5 (ginsenoside R d ).

Embodiment 2

[0021] Example 2: Chromatographic conditions: chromatographic column is RESTEK Pinnacle-C18 column (50mm-2.1mm, 5 μm); column temperature: 20°C; flow rate is 200 μL / min; mobile phase is A water (volume fraction 0.5‰ formic acid), B acetonitrile (volume fraction 0.5‰ formic acid), gradient elution (0-in, mobile phase B volume fraction 20%-50%; 13-13.1min, mobile phase B volume fraction 50%-20%; 13.1-23min, mobile phase Phase B volume fraction 20%).

[0022] Mass spectrometry conditions: electrospray ESI ion source; curtain gas 10ps; atomization gas (GAS1) 40ps; heating auxiliary gas (GAS2) 40ps; collision gas CAD medium; spray voltage IS 5500V; atomization temperature 500°C ; The detection method is positive ion multiple ion reaction detection (MRM, the ion used for quantitative analysis is m / z R g 1832.8 → 643.6; R e 969.8 → 789.7; R b 11132.1 → 365.3.

Embodiment 3

[0023] Embodiment 3: chromatographic conditions: chromatographic column: Apollo C18 chromatographic column (250 * 4.6mm, 5 μ m), mobile phase: A pump: 0.1% formic acid water; B pump: 0.1% formic acid acetonitrile, gradient program is as follows:

[0024]

[0025] Column temperature: 35°C, flow rate: 1.0ml / min (25% split into mass spectrometer), injection volume: 5μl.

[0026]Mass spectrometry conditions: first-level full scan conditions: ESI positive ion scanning, scanning mass range: 150-1000 (chloroform extraction site) and 150-1500 (n-butanol extraction site); capillary voltage: 2.8kV; cone voltage: 30V; Extraction voltage: 3.0V, lens voltage: 0.0V; source temperature: 120°C; desolvation gas flow rate and temperature are 600ml / min and 350°C, respectively; cone gas: 50ml / min. Under the above chromatographic conditions and mass spectrometry conditions, the samples from the chloroform extraction parts of Evodia rutaecarpa, the chloroform extraction parts of Evodia rutaecarp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of traditional Chinese medicine identification methodology and relates to a detection method of ginsenoside in an evodia decoction and an application thereof, specifically to an in vitro and in vivo detection method of ginsenoside and its application in the evodia decoction for treating migraine. By combination of modern HPLC and the mass spectrometric detection technology, the method has advantages of accurate result, good reproducibility and simple and fast operation, and meets requirements of biological sample analysis. With an adjuvant ginseng as the point of penetration, rat plasma pharmacokinetics of relevant components in the evodia decoction is researched, thus providing methodology reference for objectively evaluating pharmacokinetics rules of the components in human body or animal body and effects of the components in the compound prescription.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine identification methodology, and relates to a detection method and application of ginsenosides in Evodia decoction, in particular to an in vivo and in vitro detection method of ginsenosides and its effect in Evodia decoction for treating migraine. Background technique [0002] Since ancient times, ginseng has been regarded as the top grade of "happy intelligence, light body and prolonging life". It has been clinically used for thousands of years. At present, people think that the main medicinal ingredient that exerts its curative effect in the body is ginsenoside. Modern pharmacological research shows that ginsenoside It can improve microcirculation, improve tissue anti-hypoxia ability, inhibit platelet aggregation, Ca 2+ Antagonism, affecting prostaglandin metabolism, anti-tumor, anti-aging, anti-radiation and other biological activities. Base, anti-shock, anti-cerebral ischemia, prote...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 袁子悦毕开顺李清许华容胡紫霞
Owner SHENYANG PHARMA UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap