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Synchronous culture method for improving yeast biomass and intracellular trehalose content

A technology of simultaneous cultivation and biomass, applied in the field of microbial fermentation, can solve the problems of increasing the steps of centrifugal collection of yeast milk, low trehalose yield, long total fermentation time, etc., and achieves low production cost, short production cycle and convenient operation. Effect

Inactive Publication Date: 2016-08-17
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, the increase of yeast biomass and the increase of intracellular trehalose content in yeast are completed in two steps, and the step of centrifugation to collect yeast milk is added. The operation is more complicated; at the same time, the total fermentation time (22-23 h) is also longer, so seaweed Sugar yields are still low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Seed culture: Pick out Saccharomyces pentacyclicus from the activated slant medium ( Zygosaccharomyces rouxii , CCTCC NO: M 2013310) connected with 400 ml of liquid medium (100 parts of molasses with 30% sugar content, 0.5 parts of yeast extract, 1.0 parts of potassium dihydrogen phosphate, 0.05 parts of potassium chloride, 0.5 parts of ammonium sulfate, Magnesium sulfate 0.06 part) culture bottle, 30 ℃, 180 r / min, shaker culture for 24-30 h, to obtain about 400 ml of liquid seed culture solution.

[0013] (2) Fermentation culture: 4 L fermentation medium (100 parts of molasses with 30% sugar content, yeast extract 0.5 parts, 1.0 parts of potassium dihydrogen phosphate, 0.05 parts of potassium chloride, 0.5 parts of ammonium sulfate, and 0.06 parts of magnesium sulfate) were added to the fermenter at one time, and after sterilization, inoculation (the inoculation amount of yeast seed liquid should control the fermentation The initial cell concentration in the tank ...

Embodiment 2

[0016] (1) Seed culture: same as in Example 1.

[0017] (2) Fermentation culture: 4 L fermentation medium (100 parts of molasses with 30% sugar content, yeast extract 0.5 parts, 1.0 parts of potassium dihydrogen phosphate, 0.05 parts of potassium chloride, 0.5 parts of ammonium sulfate, and 0.06 parts of magnesium sulfate) were added to the fermenter at one time, and after sterilization, inoculation (the inoculation amount of yeast seed liquid should control the fermentation The initial cell concentration in the tank is 40-50 g / L), and the carbon source (molasses), nitrogen source (ammonia water), and phosphorus source (potassium dihydrogen phosphate) are fed-in. The specific feed-in method is: 1.5 L molasses ( sugar content 30%), 0.4 L ammonia water (10%, v / v), 0.3 L potassium dihydrogen phosphate (8%, w / v) from the beginning of fermentation to the completion of fermentation at about 12 h, control the flow speed, so that The specific growth rate of yeast cells was maintained...

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Abstract

The invention discloses a synchronous culture method for improving yeast biomass and intracellular trehalose content, belonging to the field of microbial fermentation. Through a synchronous culture mode of simultaneously carrying out fed-batch culture and temperature-changing culture, the method improves the yeast cell biomass as well as greatly improves the accumulation content of intracellular trehalose of yeast cells, thus remarkably improving the yield of yeast trehalose. In the method, fed-batch culture and temperature-changing culture are completed in one step, so the method has the advantages of simple process, convenient operation, short production cycle and low production cost.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for simultaneously cultivating yeast biomass and intracellular trehalose content. Background technique [0002] Trehalose is a non-reducing disaccharide that widely exists in various organisms, especially those organisms that are resistant to dehydration, such as some microorganisms, drought-tolerant plants, and even low-temperature dormant vertebrates. Because trehalose can protect biological macromolecules in cells from being damaged by harsh environments such as dryness, high temperature, freezing and high osmotic pressure, and the protective effect of trehalose is not specific, it is called "sugar of life". . Trehalose is widely used in medicine, food, cosmetics, agriculture and other fields. [0003] Trehalose production methods include: (1) Enzyme synthesis method, that is, using the transglycosylation effect of enzyme preparations to act on subs...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P19/12C12R1/645
CPCC12N1/16C12P19/12
Inventor 陈雄李欣王志代俊
Owner HUBEI UNIV OF TECH
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