Preparation method of water body denitrification material

A technology for denitrification and water body, applied in chemical instruments and methods, special compound water treatment, biological water/sewage treatment, etc. The nitrifying bacteria have strong activity, high utilization efficiency and good particle forming effect.

Inactive Publication Date: 2016-08-24
CHANGSHA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In practical applications, it was found that although the solidification microorganisms have a certain degradation effect on nitrate nitrogen and nitrite nitrogen in the water body, the removal efficiency is not high, mainly because although the solidification technology can make the substrate and metabolic reactions in the water body The product freely enters the porous gel membrane, but in the aquaculture water, other substances besides nitrate nitrogen and nitrite nitrogen can also enter the embedding body, which will adversely affect the biological function of the embedding body, thereby limiting Degradation of Nitrogen Substances in Water by Microorganisms

Method used

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  • Preparation method of water body denitrification material
  • Preparation method of water body denitrification material
  • Preparation method of water body denitrification material

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Such as figure 1 As shown, the preparation method of the water body denitrification material of the present embodiment comprises the following steps:

[0033] (1) Get the natural zeolite to grind and pulverize, then pass through a 100-mesh sieve, and the obtained sieved zeolite is immersed in 0.1mol / L hydrochloric acid solution for 2h, and then washed with deionized water until neutral;

[0034] (2) Soak the zeolite treated with hydrochloric acid in step (1) with 1 g / L lysozyme aqueous solution for 12 hours, and then wash it with deionized water for 3 times.

[0035] (3) Mix the powdery denitrifying bacteria with the zeolite treated with lysozyme in step (2) according to the mass ratio of 1:20 and then add it into the Erlenmeyer flask; weigh 4g KNO 3 , 4g NaNO 3 , 0.05g MgSO 4 ·7H 2 O, 1.85g K 2 HPO 4 , 8g potassium sodium tartrate, 1g KH 2 PO 4 , 1g FeCl 2 , 1g CaCl 2 2H 2 0, 0.5g cysteine ​​and 54.06g glucose, add deionized water to dissolve and be constant...

Embodiment 2

[0044] Such as figure 1 As shown, the preparation method of the water body denitrification material of the present embodiment comprises the following steps:

[0045] (1) Get the natural zeolite to grind and pulverize, then pass through a 100-mesh sieve, and the obtained sieved zeolite is immersed in 0.1mol / L hydrochloric acid solution for 2h, and then washed with deionized water until neutral;

[0046] (2) Soak the zeolite treated with hydrochloric acid in step (1) with 1 g / L lysozyme aqueous solution for 12 hours, and then wash it with deionized water for 3 times.

[0047] (3) Mix the powdered denitrifying bacteria with the lysozyme-treated zeolite in step (2) according to the mass ratio of 1:30 and then add it into the Erlenmeyer flask; weigh 4g KNO 3 , 4g NaNO 3 , 0.05g MgSO 4 ·7H 2 O, 1.85g K 2 HPO 4 , 8g potassium sodium tartrate, 1g KH 2 PO 4 , 1g FeCl 2 , 1g CaCl 2 2H 2 0, 0.5g cysteine ​​and 54.06g glucose, add deionized water to dissolve and be constant to ...

Embodiment 3

[0056] Such as figure 1 As shown, the preparation method of the water body denitrification material of the present embodiment comprises the following steps:

[0057] (1) Get the natural zeolite to grind and pulverize, then pass through a 100-mesh sieve, and the obtained sieved zeolite is immersed in 0.1mol / L hydrochloric acid solution for 2h, and then washed with deionized water until neutral;

[0058] (2) Soak the zeolite treated with hydrochloric acid in step (1) with 1 g / L lysozyme aqueous solution for 12 hours, and then wash it with deionized water for 3 times.

[0059] (3) Mix the powdered denitrifying bacteria with the zeolite treated with lysozyme in step (2) according to the mass ratio of 1:40 and then add it into the Erlenmeyer flask; weigh 4g KNO 3 , 4g NaNO 3 , 0.05g MgSO 4 ·7H 2 O, 1.85g K 2 HPO 4 , 8g potassium sodium tartrate, 1g KH 2 PO 4 , 1g FeCl 2 , 1g CaCl 2 2H 2 0, 0.5g cysteine ​​and 54.06g glucose, add deionized water to dissolve and be constan...

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Abstract

The invention discloses a preparation method of a water body denitrification material. The method comprises the following steps of (1) using a hydrochloric acid solution for impregnating and washing zeolite; (2) putting the zeolite into a lysozyme water solution to be soaked and washed; (3) uniformly mixing denitrifying bacteria and the zeolite, then, adding the mixture into a container, then adding a nutrition solution, introducing nitrogen gas, and performing first domestication after the sealing; (4) then, centrifuging the obtained mixture, adding centrifuging precipitate into the container, adding a nutrition solution, introducing nitrogen gas, and performing secondary domestication after the sealing; (5) centrifuging the mixture obtained in the fourth step, dripping the mixture obtained by uniformly mixing the centrifuging precipitates and algin solution into a mixed solution of a chitosan-acetic acid solution and a calcium chloride solution; stirring to form globules; then, washing the globules; (6) adding the globules into a glutaraldehyde solution; performing stirring, washing and immobilization; obtaining the water body denitrification material. The denitrification material provided by the invention has a good effect on the nitrogen substance removal from the water body.

Description

technical field [0001] The invention relates to the technical field of preparation of biological functional materials, in particular to a preparation method of a water body denitrification material. Background technique [0002] With the development of the economy, people's demand for aquatic products is increasing day by day. When the catch cannot meet the market demand, the aquaculture industry has developed rapidly. However, the current aquaculture is a resource-dependent and extensive development model. Leading to ecological imbalance, environmental degradation, shrinking resources and other problems are very prominent. The main wastes produced in the aquaculture process include residual bait, excrement, chemical substances and therapeutic drug residues. The dead bodies and pathogens of aquaculture organisms are also part of the waste. According to statistics, the excess bait accounts for 35% of these sources of pollutants. Excreta accounted for 50%, household garbage a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
CPCC02F3/34C02F2305/06
Inventor 宋勇陈圆华刘臻杨珊
Owner CHANGSHA UNIVERSITY
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