Preparation method of water body denitrification material
A technology for denitrification and water body, applied in chemical instruments and methods, special compound water treatment, biological water/sewage treatment, etc. The nitrifying bacteria have strong activity, high utilization efficiency and good particle forming effect.
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Embodiment 1
[0032] Such as figure 1 As shown, the preparation method of the water body denitrification material of the present embodiment comprises the following steps:
[0033] (1) Get the natural zeolite to grind and pulverize, then pass through a 100-mesh sieve, and the obtained sieved zeolite is immersed in 0.1mol / L hydrochloric acid solution for 2h, and then washed with deionized water until neutral;
[0034] (2) Soak the zeolite treated with hydrochloric acid in step (1) with 1 g / L lysozyme aqueous solution for 12 hours, and then wash it with deionized water for 3 times.
[0035] (3) Mix the powdery denitrifying bacteria with the zeolite treated with lysozyme in step (2) according to the mass ratio of 1:20 and then add it into the Erlenmeyer flask; weigh 4g KNO 3 , 4g NaNO 3 , 0.05g MgSO 4 ·7H 2 O, 1.85g K 2 HPO 4 , 8g potassium sodium tartrate, 1g KH 2 PO 4 , 1g FeCl 2 , 1g CaCl 2 2H 2 0, 0.5g cysteine and 54.06g glucose, add deionized water to dissolve and be constant...
Embodiment 2
[0044] Such as figure 1 As shown, the preparation method of the water body denitrification material of the present embodiment comprises the following steps:
[0045] (1) Get the natural zeolite to grind and pulverize, then pass through a 100-mesh sieve, and the obtained sieved zeolite is immersed in 0.1mol / L hydrochloric acid solution for 2h, and then washed with deionized water until neutral;
[0046] (2) Soak the zeolite treated with hydrochloric acid in step (1) with 1 g / L lysozyme aqueous solution for 12 hours, and then wash it with deionized water for 3 times.
[0047] (3) Mix the powdered denitrifying bacteria with the lysozyme-treated zeolite in step (2) according to the mass ratio of 1:30 and then add it into the Erlenmeyer flask; weigh 4g KNO 3 , 4g NaNO 3 , 0.05g MgSO 4 ·7H 2 O, 1.85g K 2 HPO 4 , 8g potassium sodium tartrate, 1g KH 2 PO 4 , 1g FeCl 2 , 1g CaCl 2 2H 2 0, 0.5g cysteine and 54.06g glucose, add deionized water to dissolve and be constant to ...
Embodiment 3
[0056] Such as figure 1 As shown, the preparation method of the water body denitrification material of the present embodiment comprises the following steps:
[0057] (1) Get the natural zeolite to grind and pulverize, then pass through a 100-mesh sieve, and the obtained sieved zeolite is immersed in 0.1mol / L hydrochloric acid solution for 2h, and then washed with deionized water until neutral;
[0058] (2) Soak the zeolite treated with hydrochloric acid in step (1) with 1 g / L lysozyme aqueous solution for 12 hours, and then wash it with deionized water for 3 times.
[0059] (3) Mix the powdered denitrifying bacteria with the zeolite treated with lysozyme in step (2) according to the mass ratio of 1:40 and then add it into the Erlenmeyer flask; weigh 4g KNO 3 , 4g NaNO 3 , 0.05g MgSO 4 ·7H 2 O, 1.85g K 2 HPO 4 , 8g potassium sodium tartrate, 1g KH 2 PO 4 , 1g FeCl 2 , 1g CaCl 2 2H 2 0, 0.5g cysteine and 54.06g glucose, add deionized water to dissolve and be constan...
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