Live animal two-photon excitation time-lapse detection fluorescence imaging analysis method and equipment

An analysis method, a technique of fluorescence imaging, which is applied in the field of in vivo fluorescence image analysis of animals, and can solve problems such as difficult application of high-efficiency two-photon excitation imaging analysis
CN105891170BActive Publication Date: 2019-07-05PEKING UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
PEKING UNIV
Publication Date
2019-07-05

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Abstract

The invention discloses a two-photon excitation time-lapse detection fluorescence imaging analysis method and equipment for living animals, which are used for analyzing the distribution and fluorescence intensity of fluorescent nano-probes (or fluorescent nano-drug-carrying analog probes) in tissues and organs in animals. Or the kinetic properties of the probe concentration changing with time, using red light or near-infrared pulsed laser two-photon to excite fluorescent nanoprobes in vivo to emit light, and detecting the fluorescence intensity of specific parts in vivo and its dynamic properties with time by time-lapse detection fluorescence imaging , has the advantages of large imaging analysis depth, high reliability and high detection sensitivity.
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Description

technical field

[0001] The present invention relates to animal in vivo fluorescence image analysis technology, in particular to a fluorescence imaging analysis method for the distribution and transport kinetic properties of nano-drug-loaded simulated probes or fluorescent nano-probes in living animals, and equipment for implementing the analysis, And the application of the method and the device in the characterization of the nano drug carrier tumor targeting kinetic properties. Background technique

[0002] Animal in vivo fluorescence imaging technology has the characteristics of high sensitivity and small damage, and has developed into a common method for imaging living animals, and is widely used in the fields of life science, biomedicine and drug research and development [V.Wagner, A.Dullaart, A. Bock, A. Zweck, The emerging nanomedicine landscape. Nature Biotechnology. 2006, 24, 1211-1217.]. In vivo fluorescence imaging systems have broad application prospects in many r...

Claims

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