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Cornea scaffold material and preparation method thereof and 3D printing method of cornea scaffold

A technology of scaffold material and cornea, which is applied in the field of implant materials and organs in medical devices, can solve the problems that patients cannot get it, donor cornea cannot regain vision, and the height of donated cornea is scarce, so as to achieve good transparency and improve degradation speed. Fast, the effect of improving mechanical strength and transparency

Inactive Publication Date: 2016-08-31
青岛三缔生物科技有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] The vast majority of blind patients with corneal stromal disease can be cured by corneal transplantation, but due to the high shortage of donated corneas, most patients cannot get donor corneas and cannot regain their sight

Method used

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  • Cornea scaffold material and preparation method thereof and 3D printing method of cornea scaffold
  • Cornea scaffold material and preparation method thereof and 3D printing method of cornea scaffold
  • Cornea scaffold material and preparation method thereof and 3D printing method of cornea scaffold

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preparation example Construction

[0022] The preparation method of this corneal stent material, such as figure 1 shown, including the following steps:

[0023] Step S1: degumming silkworm cocoons with sodium carbonate aqueous solution, dissolving them with lithium bromide aqueous solution, and then obtaining silk fibroin aqueous solution through dialysis, suction filtration and centrifugation.

[0024] Here, the mass percentage of the calcium carbonate aqueous solution is 0.5%-1%, and the molar concentration of the lithium bromide aqueous solution is 9.5±0.3mol / L.

[0025] The dialysis treatment is specifically as follows: the lithium bromide aqueous solution dissolved with degummed silkworm cocoons is dialyzed in deionized water for 72 hours with a dialysis bag with a molecular weight cut-off of 3500; during this period, the water is changed every 2 hours.

[0026] The silk fibroin aqueous solution obtained after dialysis, suction filtration and centrifugation needs to be concentrated at 10°C-78°C for 3-200 ...

Embodiment 2

[0048] Put 50g silk into 0.5% Na 2 CO 3 Degumming was carried out in the solution and dried. During degumming, the silk was boiled at 100°C for 1 hour to remove sericin on the outside of the silk, rinsed with deionized water, and the above operation was repeated 3 times, and then the silk was dried at 60°C.

[0049] Weigh 15 g of the degummed silk after the above treatment and put it into 100 mL of LiBr solution with a concentration of 9.3 mol / L for dissolving treatment, and dissolve at 60° C. for 4 hours during the dissolving treatment. Then above-mentioned LiBr solution that is dissolved with silk is carried out dialysis treatment, during dialysis, with the dialysis bag of molecular weight cut-off 3500, soak in deionized water and dialyze for 3 days, change water once every two hours during the period, to remove LiBr in the solution, thereby obtain pure Silk fibroin solution, its concentration is 6%.

[0050] Concentrate the silk fibroin solution above at 60°C for 120 hour...

Embodiment 3

[0054] Put 50g silk into 0.5% Na 2 CO 3 Degumming was carried out in the solution and dried. During degumming, the silk was boiled at 100°C for 1 hour to remove sericin on the outside of the silk, rinsed with deionized water, and the above operation was repeated 3 times, and then the silk was dried at 60°C.

[0055] Weigh 15 g of the degummed silk after the above treatment and put it into 100 mL of LiBr solution with a concentration of 9.3 mol / L for dissolution treatment, and dissolve at 60° C. for 4 hours during the dissolution treatment. Then above-mentioned LiBr solution that is dissolved with silk is carried out dialysis treatment, during dialysis, soak the dialysis bag of molecular weight cut-off 3500 in deionized water and dialyze for 3 days, change water once every two hours during the dialysis, to remove LiBr in the solution, thereby obtain pure Silk fibroin solution, its concentration is 6%.

[0056] The graphene oxide aqueous solution with a concentration of 2 mg / m...

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Abstract

The invention provides a cornea scaffold material and its preparation method and a 3D printing method of a cornea scaffold. A technical effect of preparing a cornea scaffold by a 3D printing technology is achieved. Silkworm cocoon undergoes degumming by the use of a sodium carbonate aqueous solution; the silkworm cocoon is dissolved with a lithium bromide aqueous solution, and dialysis, suction filtration and centrifugal treatment are successively carried out to obtain a concentrated silk fibroin aqueous solution, a silk fibroin nano-structure is formed and extracellular matrix structure can be simulated; a graphene oxide aqueous solution which has undergone ultrasonic dispersion treatment is mixed with the silk fibroin aqueous solution, and mass ratio of silk fibroin to graphene oxide is 10000:1-1000:1 to raise mechanical strength and transparency of the material; and a gelatin aqueous solution and a cross-linking agent are added into the obtained mixed aqueous solution, and the mixed aqueous solution is uniformly stirred at 30-50 DGE C to regulate degradation speed of the material and guarantee that the cornea scaffold can be printed. Therefore, the cornea scaffold can be printed by the 3D printing technology.

Description

technical field [0001] The invention belongs to the technical field of implant materials and organs in medical devices, and in particular relates to a corneal stent material, a preparation method thereof, and a 3D printing method of the corneal stent. Background technique [0002] The human corneal stroma accounts for 90% of the thickness of the cornea. It is mainly composed of extracellular matrix components such as corneal stromal cells and collagen fibers. It is transparent and has a certain radius of curvature, which is conducive to the passage and bending of light. Aspects such as transparency and curvature play a vital role. [0003] The vast majority of blind patients with corneal stromal disease can be cured by keratoplasty, but due to the high shortage of donated corneas, most patients cannot get donor corneas and cannot regain their sight. [0004] In recent years, 3D printing technology has been rapidly developed and applied in the preparation of 3D scaffolds for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/08A61L27/50
CPCA61L2430/16A61L27/222A61L27/08A61L27/225A61L27/50C08L89/00C08L89/06
Inventor 王红韩芳芳张楠顾敬新
Owner 青岛三缔生物科技有限公司
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