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Clean preparation method for plant polypeptide/protein

A plant polypeptide and protein technology, which is applied in the field of clean preparation of plant polypeptide/protein, can solve the problems of uncontrollable molecular weight ratio, different effects, large average molecular weight, etc., and achieve controllable molecular weight range, low viscosity and high solubility Effect

Active Publication Date: 2016-08-31
SHANDONG BOAOKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the average molecular weight of plant peptide products on the market is large, and the proportion of molecular weight in a specific range is uncontrollable. However, the mechanism and effect of peptides with different molecular weights are different, so the controllability of their functions is poor.

Method used

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  • Clean preparation method for plant polypeptide/protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of plant polypeptide using soybean meal as raw material

[0042] Proceed as follows:

[0043] (1) Raw material pretreatment: firstly, the soybean meal is tested to ensure that there is no spoilage or mold. Then, ultrasonic pretreatment is performed for 360 seconds, then crushed, passed through a 60-mesh sieve, and set aside.

[0044] (2) Slurry adjustment: Under stirring conditions, slowly add the above-mentioned crushed and sieved raw materials to a sodium hydroxide solution with a pH of 9.5, with a solid-liquid ratio of 1:10 (mass ratio).

[0045] (3) Extraction and separation: Under agitation, the temperature is raised to 55±2°C and reacted for 2.5 hours to fully extract; after the reaction, the solid-liquid separation is performed to obtain the supernatant for use.

[0046] (4) Purification: adjust the pH of the above supernatant to 5, let it stand to fully flocculate the protein, and then separate the solid-liquid to obtain crude protein, wash twice wit...

Embodiment 2

[0052] Example 2 Preparation of plant polypeptide with peanut meal as raw material

[0053] Proceed as follows:

[0054] (1) Raw material pretreatment: First, the peanut meal is tested to ensure that there is no spoilage or mold. Then, ultrasonic pretreatment is performed for 600 seconds, then crushed, passed through an 80-mesh sieve, and set aside.

[0055] (2) Slurry adjustment: Under stirring conditions, slowly add the above-mentioned crushed and sieved raw materials to an alkali solution sodium hydroxide solution with a pH of 9.5, and the solid-liquid ratio is 1:12 (mass ratio).

[0056] (3) Extraction and separation: Under agitation, the temperature is raised to 55±2°C and reacted for 3 hours to fully extract; after the reaction, solid-liquid separation is performed to obtain the supernatant for use.

[0057] (4) Purification: adjust the pH of the above supernatant to 4.5, leave it to stand to fully flocculate the protein, and then separate the solid-liquid to obtain crude protein,...

Embodiment 3

[0063] Example 3 Preparation of Plant Polypeptide with Corn Yellow Powder as Raw Material

[0064] Proceed as follows:

[0065] (1) Raw material pretreatment: Firstly, the corn yellow powder is tested to ensure that there is no spoilage or mold. Then, ultrasonic pretreatment is performed for 380 seconds, then crushed, passed through a 100-mesh sieve, and set aside.

[0066] (2) Slurry adjustment: Under stirring conditions, slowly add the above-mentioned crushed and sieved raw materials to a sodium hydroxide solution with a pH of 9.5, and a solid-to-liquid ratio of 1:8 (mass ratio).

[0067] (3) Extraction and separation: Under agitation, the temperature is raised to 55±2°C and reacted for 3 hours to fully extract; after the reaction, solid-liquid separation is performed to obtain the supernatant for use.

[0068] (4) Purification: adjust the pH of the above supernatant to 4.5, leave it to stand to fully flocculate the protein, and then separate the solid-liquid to obtain crude protein, ...

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Abstract

The invention discloses a clean preparation method for plant polypeptide / protein with a controllable molecular weight interval. The method comprises the following steps of 1, pretreatment of raw materials; 2, pulp mixing; 3, extraction and separation; 4, purification to obtain protein liquid; 5, enzymolysis, wherein the protein liquid is heated for denaturation, Na2SO3 is added, then protease is added, and protease is selected from Alcalase, neutral protease AS1398, pepsin and papain; 6, debittering of polypeptide; 7, separation through a nanofiltration membrane to obtain a polypeptide solution; 8, drying and dewatering to obtain the polypeptide product. The product prepared through the method has the controllable molecular weight interval and the advantages of high solubility, low viscosity, acid soluble stability, low bitterness and the like. The product can be widely applied to food, sport food, curative healthcare food, protein drinks, diet food and novel fermented food.

Description

Technical field [0001] The present invention relates to a clean preparation method of plant polypeptides / proteins, in particular to a preparation plant protein prepared from corn, wheat, soybeans, etc. through pretreatment, separation, and purification, and then through modern biotechnology and separation technology. A clean preparation method for prepared polypeptide products with a controllable molecular weight range. Background technique [0002] Peptides are formed by dehydration and condensation of 2 or more amino acids. They are fragments of protein structure and function, and enable proteins to have various physiological functions. They also have strong special physiological functions. By the dehydration condensation of 2 or 3 amino acids, they become dipeptides and tripeptides, and so on as tetrapeptides and pentapeptides. Generally speaking, the number of amino acids on the peptide chain is less than 10 as oligopeptides, up to 10-50 are polypeptides, and more than 50 ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/30
CPCC07K1/30C07K1/34C12P21/06
Inventor 刘钦松庄新霞刘孟刚张念强许彤陈小刚刘庆忠
Owner SHANDONG BOAOKE BIOTECH
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