Highly-active Pichia pastoris powder and preparation method thereof

A powdered Pichia pastoris, high activity technology, applied in the biological field, can solve the problems of high cost of freeze-drying, low tolerance, low number of viable bacteria, etc.

Inactive Publication Date: 2016-09-07
三门峡龙飞生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few powdered Pichia yeast powders on the market, the number of viable bacteria is low, and the stability is also poor
Mainly due to its low tolerance, it is not suitable for spray drying, but the cost of freeze drying is relatively high, which seriously restricts the industrial production of Pichia powder

Method used

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  • Highly-active Pichia pastoris powder and preparation method thereof
  • Highly-active Pichia pastoris powder and preparation method thereof
  • Highly-active Pichia pastoris powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Embodiment 1: the preparation of powdery Pichia pastoris high active bacteria powder

[0095] The implementation steps of this embodiment are as follows:

[0096] A. Activation culture

[0097] Preparation of wort juice liquid medium: According to the weight ratio of barley malt to water 1:4, add water to barley malt, stir and mix evenly, then put it in an incubator for saccharification at a temperature of 58°C for 3.8 hours, and filter to obtain The saccharified wort at a pressure of 0.5kg / cm 2 Sterilize under high temperature for 40min, then dilute the sterilized saccharified wort with water to a sugar content of 12 Baume degrees to obtain a wort liquid culture medium;

[0098] Use a sterile straw to draw 0.3mL of the above wort liquid medium, drop it into the ampoule containing freeze-dried powdered Pichia ACCC20136, shake gently to dissolve it into a suspension, absorb all the bacterial suspension, and put it in In 1L wort medium, activate and culture at 26°C for...

Embodiment 2

[0112] Embodiment 2: the preparation of powdery Pichia pastoris high active bacterial powder

[0113] The implementation steps of this embodiment are as follows:

[0114] A. Activation culture

[0115] Preparation of wort liquid culture medium: according to the weight ratio of barley malt to water 1:3, add water to barley malt, stir and mix evenly, then put it in an incubator at a temperature of 55°C for saccharification for 4.5 hours, filter to obtain The saccharified wort at a pressure of 0.6kg / cm 2 Sterilize for 38 minutes, then dilute the sterilized saccharified wort with water to a sugar content of 12 Baume degrees to obtain a wort liquid culture medium;

[0116] Use a sterile pipette to draw 0.5mL of the above wort liquid medium, drop it into the ampoule containing freeze-dried powdered Pichia ACCC20136, shake gently to dissolve it into a suspension, absorb all the bacterial suspension, and put it in In 1L wort medium, activate culture at a temperature of 30°C for 2 d...

Embodiment 3

[0130] Embodiment 3: the preparation of powdery Pichia pastoris high active bacterial powder

[0131] The implementation steps of this embodiment are as follows:

[0132] A. Activation culture

[0133] Preparation of wort liquid medium: according to the weight ratio of barley malt to water 1:5, add water to barley malt, stir and mix evenly, then put it in an incubator for saccharification at a temperature of 60°C for 3.5 hours, and filter to obtain The saccharified wort at a pressure of 0.7kg / cm 2 Sterilize under high temperature for 40min, then dilute the sterilized saccharified wort with water to a sugar content of 12 Baume degrees to obtain a wort liquid culture medium;

[0134] Use a sterile straw to draw 0.4mL of the above wort liquid medium, drop it into the ampoule containing freeze-dried powdered Pichia ACCC20136, shake gently to dissolve it into a suspension, absorb all the bacterial suspension, and put it in In 1L wort medium, activate culture at a temperature of 28...

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Abstract

The invention relates to a highly-active Pichia pastoris powder and a preparation method thereof. The preparation method comprises the steps of activation culture, primary seed culture, preparation of a high-density liquid fermentation medium, fermentation culture, material supplementing and auxiliary material addition. The preparation method can substantially the output per unit by about 30%, reduces the cost per unit by about 20%, and greatly improves the device utilization rate; a composite protection agent and a carrier maximally protect Pichia pastoris in order to obtain the highly-active Pichia pastoris powder; and compared with traditional centrifuge and spray drying production technologies, the preparation method disclosed in the invention, adopting a cold air drying technology, has the advantages of low drying temperature, satisfactory reservation of the bioactivity, overcoming of the disadvantages of low living bacteria and high investment, and wide market prospect.

Description

【Technical field】 [0001] The invention belongs to the field of biotechnology. More specifically, the present invention relates to a preparation method of high-activity powdered Pichia pastoris powder, and to the high-activity powdered Pichia pastoris powder prepared by the preparation method. 【Background technique】 [0002] Pichia powder has a very wide range of uses as an industrial strain. It can not only control the discharge of ammonia nitrogen in the production process of aquaculture, but also provide high-quality active protein for livestock and poultry to improve the quality of livestock and poultry products; some research work shows that Pichia powder can not only improve the salinity of soil effect, and has a strong inhibitory effect on root-knot nematodes, and can also increase crop yields; powdered Pichia pastoris can promote organic material decomposing, and is an important strain in the production of organic material decomposing agents; powdery Pichia pastoris ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12R1/84
CPCC12N1/16
Inventor 赵跃进赵跃文赵跃民易卿杜亚楠赵洁赵科谢松张盼任寸娟秦中辉赵九生
Owner 三门峡龙飞生物工程有限公司
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