Manufacturing method and film formation method for microfibrillated cellulose
A film-forming method and chemical fiber technology, applied in pulp beating method, non-fibrous pulp addition, fiber raw material treatment, etc., can solve the problems of high energy consumption, environmental pollution, poor product performance, etc., achieve uniform distribution, reduce energy consumption, Effect of improved optical and strength properties
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Embodiment 1
[0054] 1. Analysis of enzymatic properties
[0055] (1) Drawing of standard curve: Take 8 25ml colorimetric tubes, numbered 0~7 respectively, first add 1mg / ml standard glucose solution 0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4 into 7 respectively ml, then add buffer solutions 2.0, 1.8, 1.6, 1.4, 1.2, 1.0, 0.8, 0.6ml to tubes 0-7, then add 3ml of DNS color developing solution to each test tube, develop color in boiling water bath for 5 minutes After taking it out, put it into cold water to cool immediately, add water to make the volume to 25ml, adjust the zero point of the instrument with distilled water, measure its absorbance at 530nm, and draw a standard curve.
[0056] (2) Enzyme activity determination method: use Xinhua No. 1 filter paper (6cm×1cm, 50mg) and 50mg absorbent cotton balls as substrates, add 1ml of cellulase solution and 1ml of buffer solution with a certain pH value into the colorimetric tube , and put it into a water bath at a certain temperature to react for 60...
Embodiment 2
[0103] 1. Analysis of enzymatic properties
[0104] (1) Drawing of standard curve: Take 8 25ml colorimetric tubes, numbered 0~7 respectively, first add 1mg / ml standard glucose solution 0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4 into 7 respectively ml, then add buffer solutions 2.0, 1.8, 1.6, 1.4, 1.2, 1.0, 0.8, 0.6ml to tubes 0-7, then add 3ml of DNS color developing solution to each test tube, develop color in boiling water bath for 5 minutes After taking it out, put it into cold water to cool immediately, add water to make the volume to 25ml, adjust the zero point of the instrument with distilled water, measure its absorbance at 530nm, and draw a standard curve.
[0105] (2) Enzyme activity determination method: use Xinhua No. 1 filter paper (6cm×1cm, 50mg) and 50mg absorbent cotton balls as substrates, add 1ml of cellulase solution and 1ml of buffer solution with a certain pH value into the colorimetric tube , and put it into a water bath at a certain temperature to react for 60...
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