Composition for preventing or treating non-alcoholic liver disease or insulin resistance comprising ginsenoside F2
A non-alcoholic, ginsenoside technology, applied in the field of ginsenoside F2 pharmaceutical composition, health functional food and feed composition, can solve the problems that the effect of ginsenoside has not been revealed
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Embodiment 1
[0067] Embodiment 1. Preparation of Ginsenoside F2
[0068] The leaves and roots of other ginseng including Korean ginseng, American ginseng, and bamboo ginseng are put into 20 times the volume of 80% alcohol, extracted twice or more, and dried to obtain crude saponins. The crude saponin was redissolved in water and adsorbed to HP-20 resin, then washed with 100% water to remove sugar, and then washed once with 40% alcohol to preferentially remove protopanaxatriol (protopanaxatriol) Line of ginsenosides Re and Rg1. Thereafter, by washing with 80% alcohol, protopanaxadiol-based ginsenosides Rb1, Rb2, Rc, and Rd were eluted and dried to obtain the ginsenoside F2. The mixture of protopanaxadiol and ginsenosides was used as a substrate and reacted according to the method described in Korean Laid-Open Patent No. 2013-0134930 to obtain 70% or more of ginsenoside F2. Thereafter, if the ODS resin is used to absorb the ginsenoside F2 that will be used as a sample, and the alcohol of a...
Embodiment 2
[0069] Example 2: Confirmation of Fatty Liver Relief Effect
[0070] In order to confirm the fatty liver alleviating effect of ginsenoside F2, a mouse model fed a high-fat diet was used. Eight-week-old male mice (body weight 25-28 g) were continuously fed a high-fat diet for 12 weeks, and 250 mg / kg of ginsenoside F2 was orally administered to the mice five days a week.
[0071] Thereafter, the liver was isolated and observed, and the result was confirmed as follows: Compared with the control group, the size of the liver of the group using ginsenoside F2 ( Figure 1a ) and liver weight ( Figure 1b ) is significantly reduced. From these results, it can be seen that ginsenoside F2 has an effect of suppressing fatty liver even if a high-fat diet is performed.
Embodiment 3
[0072] Example 3: Liver tissue analysis
[0073] In order to observe the changes in the liver tissue of the mice carrying out the high-fat diet described in Example 2, perform H&E (Hematoxylin and eosin) staining, and stain the neutral lipids of the liver cells by Oil Red O (Oil Red O) staining, Express the results at a magnification of ×100 ( Figure 2a ). As a result, it was reconfirmed that the accumulation of fat in hepatocytes was reduced by the administration of ginsenoside F2.
[0074] In addition, it was confirmed that the value of neutral fat in the liver (Liver TG) decreased ( Figure 2b ), the protein expression of SREBP1c and FAS, which play an important role in fat synthesis, and the expression levels of CB1R, NAPE-PLD, SREBP1c and FAS genes decreased ( Figure 2c and Figure 2d ). Therefore, it was confirmed that ginsenoside F2 effectively suppresses fat formation in the liver.
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