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A kind of detection method of maldi-tof-ms of polythiocarbonate compound

A technology of MALDI-TOF-MS and polythiocarbonate, which is applied in the detection field of MALDI-TOF-MS, can solve the problems such as rarely seen polythiocarbonate compounds, achieve the best ionic strength and avoid drift and measurement errors, avoiding the effect of operating steps

Active Publication Date: 2019-01-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few research reports using MALDI-TOF-MS to detect polythiocarbonate compounds

Method used

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  • A kind of detection method of maldi-tof-ms of polythiocarbonate compound
  • A kind of detection method of maldi-tof-ms of polythiocarbonate compound
  • A kind of detection method of maldi-tof-ms of polythiocarbonate compound

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Embodiment 1

[0026] Embodiment 1, the MALDI-TOF-MS detection method of polythiocarbonate compound, comprises the following steps:

[0027] a) Select the sample target Ground steel stainless steel target plate;

[0028] b) Matrix solution preparation: Dithranol (DIT) was used as the matrix, dissolved in THF at a concentration of 10 mg / mL; no ionizing reagent was added;

[0029] c) The sample is made into a THF solution of 10 mg / mL;

[0030] d) Optimizing the spotting ratio: Spot the matrix solution and the sample solution at a volume ratio of 10:2, take 1 μL of the mixed solution and spot it on the sample target, and let it dry naturally to obtain a thin layer of MALDI matrix and sample crystals, and perform MALDI-TOF -MS analysis.

[0031] Mass spectrometry conditions: Ultraflex matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS) (Bruker Daltonic, Germany), positive linear mode selected, accelerating voltage 20KV, scan range of m / z2000-6000, the ...

Embodiment 2

[0034] Embodiment 2, the MALDI-TOF-MS detection method of polythiocarbonate compound, comprises the following steps:

[0035] a) Select the sample target Ground steel stainless steel target plate;

[0036] b) Preparation of matrix solution: using DIT as matrix, dissolve it in THF at a concentration of 10 mg / mL; potassium trifluoroacetate is used as the ionization reagent of the sample, and prepare a 2.5 mg / mL solution;

[0037] c) The sample is made into a THF solution of 10 mg / mL;

[0038] d) Optimizing the spotting ratio: spotting the matrix solution, sample solution and salt solution at a ratio of 10:2:0.5 (volume ratio), taking 1 μL of the mixed solution to spot on the sample target, and drying naturally to obtain MALDI matrix and sample crystallization The thin layer was analyzed by MALDI-TOF-MS.

[0039] Mass spectrometry conditions: Ultraflex matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS) (Bruker Daltonic, Germany), positiv...

Embodiment 3

[0041] Embodiment 3, the MALDI-TOF-MS detection method of polythiocarbonate compound, comprises the following steps:

[0042] a) Select the sample target Ground steel stainless steel target plate;

[0043]b) Matrix solution preparation: use DIT as the matrix, dissolve it in THF at a concentration of 10 mg / mL; use potassium trifluoroacetate as the ionization reagent of the sample, and prepare a THF solution of 2.5 mg / mL;

[0044] c) The sample is made into a THF solution of 10 mg / mL;

[0045] d) Optimizing the spotting ratio: Spotting the matrix solution, sample solution and salt solution at a ratio of 10:2:1 (volume ratio), taking 1 μL of the mixed solution to spot on the sample target, and drying naturally to obtain MALDI matrix and sample crystallization The thin layer was analyzed by MALDI-TOF-MS.

[0046] Mass spectrometry conditions: Ultraflex matrix-assisted laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS) (Bruker Daltonic, Germany), positive...

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Abstract

The present invention relates to the establishment and optimization of a matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS) analysis method for structural analysis of polythiocarbonate compounds, in particular, to a polythiocarbonate compound The detection method of MALDI-TOF-MS includes the following steps: take 1 μL of the mixed solution of matrix solution, sample solution and salt solution, spot it on the sample target, and dry it naturally to obtain a thin layer of MALDI matrix and sample crystals, and perform MALDI-TOF-MS MS analysis. The invention not only avoids complicated operation steps, reduces the loss of samples, but also improves the accuracy of analysis results, makes each peak value more accurate during mass spectrometry detection, and avoids molecular weight drift and measurement errors.

Description

technical field [0001] The present invention relates to the establishment and optimization of a matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS) analysis method for structural analysis of polythiocarbonate compounds, in particular, to a polythiocarbonate compound MALDI-TOF-MS detection method. Background technique [0002] Polycarbonate (Polycarbonate) refers to the general term for a class of polymers containing carbonate groups on the main chain, referred to as PC. Because of its high glass transition temperature, good transparency, outstanding impact resistance, electrical insulation, acid resistance, cold resistance and other advantages, it has been developed rapidly. In recent years, people have begun to seek a new type of PC with simple preparation process, environmental protection and excellent performance, and a class of polythiocarbonate compounds has emerged. The structure of a general product and its distribution affect the stabil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62
CPCG01N27/628
Inventor 刘雅琴何巧红
Owner ZHEJIANG UNIV
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