Method for extracting rutin from sophora japonica on basis of deep eutectic solvents (DESs)

A technology of deep eutectic solvent and Sophora japonica, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of high residue on ignition, high production cost, low extraction purity, etc., and achieve extraction efficiency Improve, ensure productivity, improve the effect of solubility

Active Publication Date: 2016-10-26
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Traditional extraction processes such as hot alcohol extraction, hot water extraction, cold alkaline water extraction or microwave-assisted extraction often have low extraction efficiency, large environmental pollution, high production costs, long process flow, low extraction purity, and blazing Disadvantages such as high residue on ignition

Method used

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  • Method for extracting rutin from sophora japonica on basis of deep eutectic solvents (DESs)
  • Method for extracting rutin from sophora japonica on basis of deep eutectic solvents (DESs)
  • Method for extracting rutin from sophora japonica on basis of deep eutectic solvents (DESs)

Examples

Experimental program
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Effect test

Embodiment 1

[0031] (1) Preparation of deep eutectic solvent (ChCl / triethylene glycol). After drying choline chloride (ChCl) with a purity of 99% in a vacuum oven at 50°C for 48 hours, mix it with triethylene glycol at a molar ratio of 1:4, and place it in a 50mL container with a magnet Put a stopper in a round-bottomed flask, seal it with a sealing film, place it in an oil bath at 100°C, stir and heat at 500rpm for about 2 to 4 hours, until a stable, uniform, and transparent solution is formed. Cool to room temperature, dry in a vacuum oven at 70°C for 48 hours, and then transfer to a desiccator for sealed storage.

[0032] (2) Sophora japonica pretreatment. Dry the purchased Sophora japonica in the air for 48 hours, pulverize it with a traditional Chinese medicine grinder, and pass it through a 60-mesh sieve. Sophora japonica fine powder is bagged and stored in a desiccator at room temperature.

[0033] (3) Extraction of rutin. Weigh Sophora japonica fine powder, add DES prepared in ...

Embodiment 2

[0036] (1) Preparation of deep eutectic solvent (ChCl / triethylene glycol). After drying choline chloride (ChCl) with a purity of 99% in a vacuum oven at 50°C for 48 hours, mix it with triethylene glycol at a molar ratio of 1:3, and place it in a 50mL container with magnets placed in advance. Put a stopper in a round-bottomed flask, seal it with a sealing film, place it in an oil bath at 100°C, stir and heat at 500rpm for about 2 to 4 hours, until a stable, uniform, and transparent solution is formed. Cool to room temperature, dry in a vacuum oven at 70°C for 48 hours, and then transfer to a desiccator for sealed storage.

[0037] (2) Sophora japonica pretreatment. With embodiment 1.

[0038] (3) Extraction of rutin. With embodiment 1. The amount of rutin extracted under these conditions was 245mg / g.

[0039] (4) Separation and purification of rutin. With embodiment 1.

Embodiment 3

[0041] (1) Preparation of deep eutectic solvent (ChCl / triethylene glycol). Choline chloride (ChCl) with a purity of 99% was dried in a vacuum oven at 50°C for 48 hours, then mixed with triethylene glycol at a molar ratio of 1:5, and placed in a 50mL container with a magnet Put a stopper in a round-bottomed flask, seal it with a sealing film, place it in an oil bath at 100°C, stir and heat at 500rpm for about 2 to 4 hours, until a stable, uniform, and transparent solution is formed. Cool to room temperature, dry in a vacuum oven at 70°C for 48 hours, and then transfer to a desiccator for sealed storage.

[0042] (2) Sophora japonica pretreatment. With embodiment 1.

[0043] (3) Extraction of rutin. With embodiment 1. The amount of rutin extracted under these conditions was 244mg / g.

[0044] (4) Separation and purification of rutin. With embodiment 1.

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Abstract

The invention belongs to the technical field of extraction processes, and discloses a method for extracting rutin from sophora japonica on the basis of deep eutectic solvents (DESs). The method comprises the following steps: heating aqueous deep eutectic solvents (DESs) and sophora japonica with stirring to perform extraction so as to obtain an extracting solution, and carrying out separation and purification to obtain the rutin. By utilizing the DESs obtained by mixing choline chloride and triglycol to perform extraction, since the DESs can severely damage the cell walls of the natural product sophora japonica, the target product rutin is sufficiently released, thereby obviously enhancing the solubility of the rutin in the solvent, and obviously enhancing the extraction efficiency as compared with the traditional extraction solvent. The DESs are easy to prepare, have the advantages of low volatilization tendency, high heat stability, low melting point and the like, and can lower the air pollution and the human body injury to the production personnel caused by solvent volatilization to some extent. The method has the advantages of simple operating technique, mild conditions, environment friendliness and the like.

Description

technical field [0001] The invention belongs to the technical field of extraction technology, and in particular relates to a method for extracting rutin from Sophora japonica based on a deep eutectic solvent. Background technique [0002] Rutin, also known as rutin, 3',4',5,7-tetrahydroxyflavone-3-rutinoside, has the following structural formula: [0003] [0004] Rutin is a natural flavonoid, the main component of vitamin P. Sophora japonica is native to northern my country, and is also produced in South China and Southwest China. Hebei Province, Shanxi Province and Beijing have abundant production, and Jiangsu is mainly produced in Zhenjiang, Suzhou, Nanjing, Xuzhou and other places. Rutin has the functions of reducing capillary permeability, maintaining normal osmotic pressure of blood vessels, maintaining or restoring normal elasticity of capillaries, and has strong pharmacological activity. As my country's population structure tends to age, the demand for drugs fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 娄文勇赵冰怡宗敏华陈庆生华洋林杨继国
Owner SOUTH CHINA UNIV OF TECH
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