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Kit for fungal rapid culture, identification and drug sensitivity

A kit and drug susceptibility technology, which is applied in the field of fungal rapid culture, identification and drug susceptibility kits, can solve the problems of long detection and culture time and poor identification, and achieve strong clinical adaptability, treatment and clinical detection. Therapeutic, specific effect

Inactive Publication Date: 2016-10-26
郑州点石生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a kit for rapid fungal culture, identification and drug sensitivity, which solves the problems of long cultivation time and poor identification pertinence in the existing fungal drug sensitivity detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This embodiment provides a kit for rapid culture, identification and drug sensitivity of fungi, including a culture solution, a diluent, liquid paraffin oil and a detection plate, and the culture solution contains 0.3% tryptone, 0.8% NaCl, NaCl 2 HPO 4 0.05%, Nichloramine 0.08%, Ammonium Sulfate 0.1%, Standard Calf Serum 30.0%, Ferrous Sulfate 0.001%, Potassium Chloride 0.05%, Egg Yolk Liquid 5.0%, Sucrose 1.5%, Glucose 0.1%, Vancomycin 0.001 %, chloramphenicol 0.08%, and methylbromide purple 0.05%; the detection plate is provided with fungal identification holes and antibiotic susceptibility detection holes.

[0023] Wherein, the diluent is an aqueous NaCl solution with a concentration of 0.8%.

[0024] Wherein, the fungal identification hole includes more than one fungal identification hole in Candida albicans, Candida tropicalis, Candida krusei, Candida glabrata, and Cryptococcus neoformans.

[0025] Wherein, the antibiotic sensitivity detection group includes sev...

Embodiment 2

[0028] The present embodiment provides a kit for rapid culture, identification and drug sensitivity of fungi, including a culture solution, a diluent, liquid paraffin oil and a detection plate, and the culture solution contains 1.0% tryptone, 0.7% NaCl, NaCl 2 HPO 4 0.2%, Nichloramine 0.05%, Ammonium Sulfate 0.15%, Standard Calf Serum 20%, Ferrous Sulfate 0.003%, Potassium Chloride 0.03%, Egg Yolk Liquid 10.0%, Sucrose 1.0%, Glucose 0.3%, Vancomb 0.001% chloramphenicol, 0.05% chloramphenicol, and methyl bromide violet 0.1%; fungal identification holes and antibiotic susceptibility detection holes are arranged on the detection plate.

[0029] Wherein, the diluent is an aqueous NaCl solution with a concentration of 0.5%.

[0030] Wherein, the fungal identification hole includes more than one fungal identification hole in Candida albicans, Candida tropicalis, Candida krusei, Candida glabrata, and Cryptococcus neoformans.

[0031] Wherein, the antibiotic sensitivity detection g...

Embodiment 3

[0034] This embodiment provides a kit for rapid culture, identification and drug susceptibility of fungi, including culture fluid, diluent, liquid paraffin oil, and detection plate. The culture fluid contains 2.0% tryptone, 0.6% NaCl, NaCl 2 HPO 4 0.4%, Nichloramine 0.03%, Ammonium Sulfate 0.2%, Standard Calf Serum 10.0%, Ferrous Sulfate 0.005%, Potassium Chloride 0.01%, Egg Yolk Liquid 15.0%, Sucrose 0.5%, Glucose 0.5%, Vancomycin 0.001% chloramphenicol, 0.01% chloramphenicol, and methyl bromide violet 0.02%; the detection plate is provided with fungal identification holes and antibiotic susceptibility detection holes.

[0035] Wherein, the diluent is an aqueous NaCl solution with a concentration of 1.0%.

[0036] Wherein, the fungal identification hole includes more than one fungal identification hole in Candida albicans, Candida tropicalis, Candida krusei, Candida glabrata, and Cryptococcus neoformans.

[0037] Wherein, the antibiotic sensitivity detection group includes...

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PUM

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Abstract

The invention discloses a kit for fungal rapid culture, identification and drug sensitivity, and belongs to the technical field of drug sensitivity detection. The kit includes a culture liquid, a diluent, mineral oil and a detection plate; the culture liquid contains tryptone, NaCl, Na2HPO4, dicloran, ammonium sulfate, a standard fetal bovine serum, ferrous sulfate, potassium chloride, an egg yolk liquid, sucrose, glucose, vancomycin, chloramphenicol, and bromocresol purple; the detection plate is provided with a fungal identification hole and an antibiotic drug sensitivity detection hole. According to the kit, sucrose and glucose efficient growth factors and other effective components are added in the used culture liquid, so that the rapid growth of fungi can be guaranteed, culture of the fungi is completed in 24 h, a result can be obtained in 24 h, the period of clinical detection is greatly shortened, and early diagnosis and treatment of patients are facilitated.

Description

technical field [0001] The invention relates to the technical field of drug sensitivity detection, in particular to a kit for rapid culture, identification and drug sensitivity of fungi. Background technique [0002] Fungi (fungus) originally belonged to the plant kingdom, but the modern classification system separates them into a kingdom. Fungi, unlike bacteria, have a true nuclear envelope, produce spores, lack chlorophyll, produce branched hyphae, and have a cell wall of chitin or cellulose. Clinically, pathogenic fungi are divided into two types: superficial fungi and deep fungi. The former invades the skin, hair, and nails and is obstinate to treatment, but has less impact on the body. The latter can invade the internal organs of the whole body, and severe cases can cause death. Deep fungi are fungi that invade the subcutaneous tissue and internal organs and cause systemic infections. These fungi are mainly Candida and Cryptococcus neoformans. Candida widely exists i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12Q1/18
CPCC12Q1/04C12Q1/18
Inventor 李杰
Owner 郑州点石生物技术有限公司
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