Bladder acellular matrix-cell-three-dimensional fiber network based bionic tissue and preparation method thereof
An acellular matrix and fiber network technology, which is applied in the field of composite bladder acellular matrix hydrogel preparation, can solve the problems of excessively fast component migration and insufficient structural strength, and achieve the effects of good biocompatibility and biodegradability
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Embodiment 1
[0092] Based on the preparation method of bladder acellular matrix-cell-three-dimensional fiber network biomimetic tissue, the acellular matrix material from the bladder is prepared, freeze-dried, then freeze-ground, crushed into powder; digested with pepsin in hydrochloric acid solution Bladder decellularized matrix powder for about 48 hours to form bladder decellularized matrix pregel solution, add about 1 / 10 of the volume of bladder decellularized matrix pregel solution NaOH, wherein the concentration of NaOH is 0.01mol / L, then add about 1 / 10 9 volumes of bladder acellular matrix pregel solution volume 10×PBS, wherein the concentration of PBS is 0.01mol / L, then add insulin, wherein the mass ratio of insulin to bladder acellular matrix is 0.05:100, to form bladder acellular matrix gel precursor solution. Before the gel was formed, the above-mentioned bladder acellular matrix precursor solution was coated on the fibers prepared by the three-dimensional weaving method with a...
Embodiment 2
[0094] Based on the preparation method of bladder acellular matrix-cell-three-dimensional fiber network biomimetic tissue, the acellular matrix material from the bladder is prepared, freeze-dried, then freeze-ground, crushed into powder; digested with pepsin in hydrochloric acid solution Bladder decellularized matrix powder for about 48 hours to form bladder decellularized matrix pregel solution, add about 1 / 10 of the volume of bladder decellularized matrix pregel solution NaOH, wherein the concentration of NaOH is 0.01mol / L, then add about 1 / 10 9 volumes of bladder acellular matrix pregel solution volume 10×PBS, wherein the concentration of PBS is 0.01mol / L, then add aspirin, wherein the mass ratio of aspirin to bladder acellular matrix is 1:20, to form bladder acellular matrix gel precursor solution. Before the gel was formed, the above-mentioned bladder acellular matrix precursor solution was sprayed on the fibers prepared by the three-dimensional weaving method with a di...
Embodiment 3
[0096] Preparation method of biomimetic tissue based on bladder acellular matrix-cell-three-dimensional fiber network: prepare the acellular matrix material from the bladder, freeze-dry it, freeze and grind it, and break it into powder; digest it with pepsin in hydrochloric acid solution The acellular matrix powder is about 48 hours to form a bladder acellular matrix pregel solution, add about 1 / 10 of the volume of the bladder acellular matrix pregel solution NaOH, wherein the concentration of NaOH is 0.01mol / L, and then add about 1 / 9 Bladder decellularized matrix pregel solution volume 10×PBS, wherein the concentration of PBS is 0.01mol / L, then add ketoprofen, wherein the mass ratio of ketoprofen to bladder decellularized matrix is 1:10, to form decellularized bladder Matrix gel precursor solution. Before the gel was formed, the above-mentioned bladder acellular matrix precursor solution was coated on the fibers prepared by the three-dimensional weaving method with a diamet...
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