Bladder acellular matrix-cell-three-dimensional fiber network based bionic tissue and preparation method thereof

An acellular matrix and fiber network technology, which is applied in the field of composite bladder acellular matrix hydrogel preparation, can solve the problems of excessively fast component migration and insufficient structural strength, and achieve the effects of good biocompatibility and biodegradability

Active Publication Date: 2016-11-09
DONGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a bionic tissue based on bladder acellular matrix-cell-three-dimensional fiber network and its preparation method, which is a bladder acellular matrix bionic tissue and its preparation method for urinary system repair, to solve The technical problems of insufficient structural strength of the existing technology and excessively fast migration speed of internal effective additives

Method used

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  • Bladder acellular matrix-cell-three-dimensional fiber network based bionic tissue and preparation method thereof
  • Bladder acellular matrix-cell-three-dimensional fiber network based bionic tissue and preparation method thereof
  • Bladder acellular matrix-cell-three-dimensional fiber network based bionic tissue and preparation method thereof

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Embodiment 1

[0092] Based on the preparation method of bladder acellular matrix-cell-three-dimensional fiber network biomimetic tissue, the acellular matrix material from the bladder is prepared, freeze-dried, then freeze-ground, crushed into powder; digested with pepsin in hydrochloric acid solution Bladder decellularized matrix powder for about 48 hours to form bladder decellularized matrix pregel solution, add about 1 / 10 of the volume of bladder decellularized matrix pregel solution NaOH, wherein the concentration of NaOH is 0.01mol / L, then add about 1 / 10 9 volumes of bladder acellular matrix pregel solution volume 10×PBS, wherein the concentration of PBS is 0.01mol / L, then add insulin, wherein the mass ratio of insulin to bladder acellular matrix is ​​0.05:100, to form bladder acellular matrix gel precursor solution. Before the gel was formed, the above-mentioned bladder acellular matrix precursor solution was coated on the fibers prepared by the three-dimensional weaving method with a...

Embodiment 2

[0094] Based on the preparation method of bladder acellular matrix-cell-three-dimensional fiber network biomimetic tissue, the acellular matrix material from the bladder is prepared, freeze-dried, then freeze-ground, crushed into powder; digested with pepsin in hydrochloric acid solution Bladder decellularized matrix powder for about 48 hours to form bladder decellularized matrix pregel solution, add about 1 / 10 of the volume of bladder decellularized matrix pregel solution NaOH, wherein the concentration of NaOH is 0.01mol / L, then add about 1 / 10 9 volumes of bladder acellular matrix pregel solution volume 10×PBS, wherein the concentration of PBS is 0.01mol / L, then add aspirin, wherein the mass ratio of aspirin to bladder acellular matrix is ​​1:20, to form bladder acellular matrix gel precursor solution. Before the gel was formed, the above-mentioned bladder acellular matrix precursor solution was sprayed on the fibers prepared by the three-dimensional weaving method with a di...

Embodiment 3

[0096] Preparation method of biomimetic tissue based on bladder acellular matrix-cell-three-dimensional fiber network: prepare the acellular matrix material from the bladder, freeze-dry it, freeze and grind it, and break it into powder; digest it with pepsin in hydrochloric acid solution The acellular matrix powder is about 48 hours to form a bladder acellular matrix pregel solution, add about 1 / 10 of the volume of the bladder acellular matrix pregel solution NaOH, wherein the concentration of NaOH is 0.01mol / L, and then add about 1 / 9 Bladder decellularized matrix pregel solution volume 10×PBS, wherein the concentration of PBS is 0.01mol / L, then add ketoprofen, wherein the mass ratio of ketoprofen to bladder decellularized matrix is ​​1:10, to form decellularized bladder Matrix gel precursor solution. Before the gel was formed, the above-mentioned bladder acellular matrix precursor solution was coated on the fibers prepared by the three-dimensional weaving method with a diamet...

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Abstract

The invention relates to a bladder acellular matrix-cell-three-dimensional fiber network based bionic tissue and a preparation method thereof, and specifically relates to a bladder acellular matrix-cell-three-dimensional fiber network based bionic tissue for repairing a urinary system and a preparation method thereof. The bladder acellular matrix-cell-three-dimensional fiber network based bionic tissue at least comprises composite bladder acellular matrix hydrogel and epithelial cells; the composite bladder acellular matrix hydrogel is bladder acellular matrix hydrogel with a three-dimensional fiber network material having good biocompatibility as a framework; the three-dimensional fiber network material having good biocompatibility is a network structure material formed by winding fiber with good biocompatibility. The bladder acellular matrix-cell-three-dimensional fiber network based bionic tissue provided by the invention can perform simulation according to the structure of a bladder, and has a good application prospect in the urinary system.

Description

technical field [0001] The invention belongs to the technical field of preparation of composite bladder acellular matrix hydrogel, and relates to a bionic tissue based on bladder acellular matrix-cell-three-dimensional fiber network, in particular to bladder acellular matrix-cell-three-dimensional fiber network for urinary system repair Biomimetic organization of fiber network and method for its preparation. Background technique [0002] At present, the repair and reconstruction of the urinary system mainly includes the bladder and urethra. The repair and reconstruction of complex bladder and urethra is still one of the most difficult problems in urology. It is very important to select suitable substitute materials for bladder and urethra reconstruction in the treatment of neurogenic bladder, urethral stricture and other diseases. At present, in the repair and reconstruction of the urinary system, a variety of autologous tissue materials are mainly used, such as penile or p...

Claims

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Application Information

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IPC IPC(8): A61L27/52A61L27/50A61L27/36A61L27/56A61L27/54A61L27/28A61L27/22A61L27/18
Inventor 张耀鹏宋鲁杰黄建文邵惠丽傅强蒋丹胡学超
Owner DONGHUA UNIV
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