A method for extracting tea saponin from camellia oleifera seed dregs

Abstract

The invention discloses a method for extracting tea saponin from camellia oleosa seed meal, and belongs to the field of tea saponin extraction. The method comprises the steps that the camellia oleosa seed meal is taken as raw materials to be pulverized and then sequentially subjected to ethyl acetate leaching degreasing and enzyme treating, so that effective ingredients in cells of the camellia oleosa seed meal are fully released; ultrasonic extraction is conducted through cooperation of ethyl alcohol, and urea is utilized to cooperate with dry ice for cooling and recrystallizing, so that tea saponin precipitates; the precipitates are heated, so that residual ethyl alcohol is volatilized and urea is decomposed, and the high-purity tea saponin solid is finally obtained. According to the method, the obtained tea saponin is free of organic solvent residues, the urea can be completely decomposed to be taken away by nitrogen flow after being heated, and the purity of the obtained tea saponin can reach 98% or above.

Description

technical field [0001] The invention discloses a method for extracting tea saponin from camellia oleifera seed dregs, belonging to the field of tea saponin extraction. Background technique [0002] Camellia oleifera seeds of camellia oil tree can be pressed to obtain high-quality edible oil. The oleic acid content of camellia oleifera seeds is as high as 86%, and the linoleic acid content is 7-10%, which can be compared with olive oil. Camellia oleifera is mostly distributed in the southern region. China produces 1 million tons of camellia oleifera seeds per year, and the output of the remaining camellia oleifera seed meal is about 690,000 tons per year, and it shows an increasing trend every year. [0003] In ancient times, tea oil was obtained by pressing tea seeds at high temperature, and tea seed meal can be used as shampoo, mosquito coils, soap, etc. Tea seed meal is rich in nutrients, such as residual oil, protein, tea saponin, crude fiber and sugar, and can be widely...

AI Technical Summary

Problems solved by technology

[0006] The technical problem that the present invention mainly solves: for the traditional tea saponin that occurs in the extraction process, the yield and purity of tea saponin are low during water immersion extraction, which limits the application range of tea saponin, and during organic solvent extraction, The methanol used is poisonous. Although the purity of ethanol extraction is improved compared with the water extraction method, the problem of high purity cannot be achieved. A method for extracting tea saponin from camellia oleifera seed dregs is provided. The present invention uses camellia oleifera seed dregs as raw material. After pulverization, leaching and defatting with ethyl acetate, and then treating with enzymes to fully release the active ingredients in the cells, then using ethanol and ultrasonic extraction, and then using urea and dry ice to cool down and recrystallize to precipitate tea saponin. Heat the precipitate to volatilize the residual ethanol, decompose the urea, and finally obtain high-purity tea saponin solid