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Method for extracting gutta-percha from leaves and bark of eucommia ulmoides

A technology of eucommia ulmoides leaves and eucommia gum, which is applied in the field of natural product extraction, can solve the problems of unfavorable eucommia gum refining, long fermentation time, and low gum purity, and achieve the effects of increasing solubility and mobility, less chemical reagents, and simple processes

Active Publication Date: 2016-11-09
广州存正生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, people such as Lu Zhike adopt sweet wine koji to ferment Eucommia leaves and adopt 5% NaOH solution to extract Eucommia gum after 90~100 DEG C of leaching fermentation, but the fermentation time is long, and the purity of the gum extracted is low; solvent method ( That is, the toluene method) is to use 2% NaOH solution to extract Eucommia leaves at 90-100 ° C, then use toluene to reflux to extract Eucommia leaves, and then extract the toluene reflux extract with 5% NaOH solution to obtain Eucommia gum. The loss of gum silk is small and the purity is high, but it needs repeated distillations to remove the solvent, which is not conducive to the refining of Eucommia gum
The relatively clean microbial method (Chinese patent: CN101914211A) is used to extract Eucommia gum, the disadvantage is that the cycle is long and the cost is high

Method used

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  • Method for extracting gutta-percha from leaves and bark of eucommia ulmoides

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The method for extracting Eucommia gum in a kind of Eucommia leaf bark that embodiment 1 provides, comprises the steps:

[0027] Clean the eucommia leaf bark, dry and mechanically crush it, soak it in 0.1% NaOH at room temperature for 24 hours, wash and filter it with clean water, filter the obtained filter residue, the pH value of the rinsed filtrate is 8, sterilize the filter residue by high-temperature steam at 200°C, and add 0.05% Aqueous solution of polyethylene glycol octylphenyl ether adjusts the moisture content of the filter residue to 60%, and then inoculates Streptomyces strains, the strain quality is 3% of the dried mass of Eucommia bark leaves, and 0.5% corn flour and 1% urea are added , the initial pH was controlled at 8, the temperature was controlled at 40°C, and fermentation was carried out for 8 days; petroleum ether was added to the fermented Eucommia leaf bark to extract, the ratio of the volume of petroleum ether to the dried mass of Eucommia bark an...

Embodiment 2

[0029] The method for extracting eucommia gum in a kind of eucommia leaf bark that embodiment 2 provides, comprises the steps:

[0030] Clean the eucommia leaf bark, dry and mechanically crush it, soak it in 1% NaOH at room temperature for 12 hours, wash and filter it with clean water, filter the obtained filter residue, the pH value of the rinsed filtrate is 9, sterilize the filter residue with high-temperature steam at 100°C, and add 0.15% The sorbitan monostearate aqueous solution adjusts the water content of the filter residue to 65%, and then inoculates the Streptomyces strain, the strain quality is 5% of the dried quality of Eucommia bark leaves, and adds 0.5% corn flour and 1% urea, The initial pH was controlled at 9, the temperature was controlled at 35°C, and fermentation was carried out for 8 days; the fermented eucommia leaf bark was added to petroleum ether for extraction, the ratio of the volume of petroleum ether to the dried mass of eucommia bark and leaves was 1...

Embodiment 3

[0032] The method for extracting Eucommia gum in a kind of Eucommia leaf bark that embodiment 3 provides, comprises the steps:

[0033] Wash, dry and mechanically crush the leaves of Eucommia ulmoides, soak them in 0.5% NaOH at room temperature for 18 hours, rinse and filter with clean water, filter the obtained filter residue, the pH value of the rinse filtrate is 8.5, sterilize the filter residue with high-temperature steam at 120°C, add 0.1 % polyoxyethylene dehydrated monolaurate aqueous solution to adjust the moisture content of the filter residue to 70%, then inoculate Streptomyces strains, the strain quality is 4% of the dried mass of Eucommia bark leaves, and add 0.5% corn flour and 1% urea , the initial pH was controlled at 8.5, the temperature was controlled at 38°C, and fermentation was carried out for 8 days; petroleum ether was added to the fermented Eucommia leaf bark to extract, the ratio of the volume of petroleum ether to the dried mass of Eucommia bark was 14:...

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Abstract

The invention discloses a method for extracting gutta-percha from the leaves and bark of eucommia ulmoides. The method comprises the steps that the leaves and bark of the eucommia ulmoides are washed, dried and mechanically crushed, soaking is carried out with aqueous alkali at normal temperature, filtering is carried out, and filter residues are subjected to high temperature sterilization; an aqueous surfactant solution is added to adjust the moisture content of the filter residues, then the filter residues are inoculated with actinomycetes strains, a carbon source and a nitrogen source are added, and fermentation is carried out to damage cellulose in the leaves and bark of the eucommia ulmoides; then the gutta-percha is extracted through a conventional solvent method. Through aqueous alkali pretreatment on the gutta-percha, the leaves and bark of the eucommia ulmoides are fermented through actinomycetes in an alkali environment to degrade lignin, meanwhile, and the aqueous surfactant solution is added to improve the degradation efficiency of the actinomycetes on the cellulose in the leaves and bark of the eucommia ulmoides. According to the method, the gutta-percha yield is increased, and meanwhile the efficiency of extracting the gutta-percha through a biodegradation method is improved.

Description

【Technical field】 [0001] The invention belongs to the field of natural product extraction and relates to a method for extracting eucommia gum from eucommia leaf bark. 【Background technique】 [0002] Eucommia ulmoides (scientific name Eucommia ulmoides Oliver), also known as Bakelite, is a plant of Eucommia ulmoides. At present, the cultivation area of ​​Eucommia ulmoides in China is about 360,000 hm 2 , accounting for more than 99% of the total planting area of ​​Eucommia ulmoides in the world, and has unique resource advantages. Studies have shown that the main active ingredients of Eucommia ulmoides are lignin, phenylpropanoids, cyclic ethers, flavonoids, polysaccharides, amino acids, eucommia gum, and various trace elements and vitamins. Eucommia ulmoides is a unique economic crop in China, and it is also a precious Chinese medicinal material, and it is also a high-quality gum source tree species with the greatest development potential in the world. Eucommia gum is the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/00C08L7/00
CPCC08J3/00C08J2307/00
Inventor 曹蕊
Owner 广州存正生物科技有限公司
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