Selenium-rich glossy ganoderma hypha powder and a preparing method thereof

A technology of Ganoderma lucidum mycelium and mycelium powder, applied in biochemical equipment and methods, microorganism-based methods, fungi and other directions, can solve the problems of high price, low selenium enrichment rate of mycelium, high production cost, and achieve stable quality Safety, improving the enrichment rate of selenium, and reducing the production cost

Inactive Publication Date: 2016-11-09
湖南中医药高等专科学校
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0006] The applicant has long been committed to the cultivation of selenium-enriched Ganoderma lucidum mycelia, and published in the magazine "Edible Fungi" (2014 (6) pages 7-9) in 2014, titled "Ganoderma lucidum liquid selenium-enriched deep layer culture time and mycelia yield and quality The relationship between" mentioned in the paper that pretreated corn flour, nutrient broth, potassium dihydrogen phosphate, magnesium sulfate, vitamin C, diselenose sugar and purified water were used as raw materials to prepare a liquid medium. Optimal quality and improved yield of Ganoderma lucidum mycelia were obtained, but the obtained mycelia had a low selenium-enrichment rate, and the relatively expensive animal protein nutrient broth was used as a nitrogen source, resulting in high production costs

Method used

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  • Selenium-rich glossy ganoderma hypha powder and a preparing method thereof
  • Selenium-rich glossy ganoderma hypha powder and a preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Prepare each raw material according to the amount required for the selenium-enriched ganoderma mycelium powder medium shown in Table 1, and the preparation method of the selenium-enriched ganoderma mycelium powder comprises the following steps:

[0042] S1. Preparation of selenium-enriched Ganoderma lucidum mycelia powder medium

[0043] S11. Pretreatment of corn flour: first add corn flour to 4 times the amount of purified water, gelatinize at 90°C for 15 minutes, and cool to room temperature; then add liquefaction enzyme, the mass ratio of corn flour to liquefaction enzyme is 25:2, Liquefaction at constant temperature for 4 hours, boil at 100°C for 20 minutes, cool to room temperature; then add glucoamylase, the mass ratio of corn flour to glucoamylase is 25:1, saccharify at 60°C for more than 12 hours, boil at 100°C for 20 minutes, and cool to room temperature; Then at a rotating speed of 800r / min, centrifuge for 15min to filter, get the filtrate to obtain corn flour...

Embodiment 2、3

[0049] Different from Example 1, various raw materials were prepared as shown in Table 1.

[0050] The biomass of the prepared selenium-enriched ganoderma lucidum mycelium powder, the determination of the polysaccharide content and the selenium content in the obtained selenium-enriched ganoderma lucidum mycelium powder were determined, and the results are shown in Table 2.

Embodiment 4

[0052] Different from Example 1, prepare selenium-enriched Ganoderma lucidum mycelium powder according to the following steps:

[0053] S1. Preparation of selenium-enriched Ganoderma lucidum mycelia powder medium

[0054] S11. Pretreatment of corn flour: first add corn flour to 1 times the amount of purified water, gelatinize at 90°C for 15 minutes, and cool to room temperature; then add liquefaction enzyme, the mass ratio of corn flour to liquefaction enzyme is 25:1, Liquefaction at constant temperature for 4 hours, boil at 100°C for 20 minutes, cool to room temperature; then add glucoamylase, the mass ratio of corn flour to glucoamylase is 25:1.5, saccharify at 60°C for more than 12 hours, boil at 100°C for 20 minutes, and cool to room temperature; Then at a rotating speed of 800r / min, centrifuge for 15min to filter, get the filtrate to obtain corn flour liquid, and set aside;

[0055] S12. Pretreatment of soybean cake: take soybean cake and extract with 1 times the amount ...

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Abstract

Selenium-rich glossy ganoderma hypha powder and a preparing method thereof are provided. Through adopting biological theories as guidance, the hypha powder is prepared by preparing a liquid medium by adopting corn flour, soybean cakes, cane sugar, glucose, monopotassium phosphate and organic-state diselenium-sugar as additives, sterilizing, cooling, adding a cultured strain seed liquid, culturing in an oscillator or a liquid fermentation tank at 28 DEG C under high oxygen flux conditions for about two weeks with the oscillation rate being 180 times per min, filtering and drying. The selenium content of the hypha powder prepared by the method is greatly increased, and the hypha powder is low in production cost and high in yield. The organic-state selenium is stable and effective. A product of the method is high in nutrient contents, safe and reliable. The hypha powder and the method are suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of cultivation of ganoderma lucidum mycelium powder, and more particularly relates to a selenium-enriched ganoderma mycelium powder and a preparation method thereof. Background technique [0002] With the advent of urbanization, industrialization, and population aging, environmental pollution has intensified, agricultural ecological conditions have deteriorated, and the safety of various foods has declined, posing a serious threat to human survival. In the past 30 years, the spectrum of human diseases and causes of death have undergone fundamental changes. According to statistics, cardiovascular and cerebrovascular diseases, malignant tumors, diabetes and other metabolic diseases have the highest impact on human health, accounting for more than 70% of the mortality rate. . [0003] Selenium is one of the essential trace elements for the human body and has important physiological functions. It is known as "f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 张旺凡
Owner 湖南中医药高等专科学校
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