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Method for detecting lomefloxacin enantiomer in aquatic product

A technology for aquatic products and enantiomers, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of high cost, long separation time, and complicated operation of lomefloxacin enantiomers, and achieve the optimization of solid-phase extraction conditions, The effect of good linear relationship and simple operation

Inactive Publication Date: 2016-11-09
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a fast, high precision, simple operation and low cost in order to solve the defects of high cost, long separation time, narrow applicability, complicated operation and low precision of lomefloxacin enantiomer detected by the existing crystallization method. Lower method for detection of lomefloxacin enantiomers in aquatic products

Method used

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  • Method for detecting lomefloxacin enantiomer in aquatic product

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Embodiment 1

[0026] A method for detecting lomefloxacin enantiomers in aquatic products, comprising the following steps:

[0027] 1) Use a mixture of isopropanol and n-heptane with a volume ratio of 90%: 10% as the mobile phase to dissolve and dilute lomefloxacin to a solution of 50 μg / mL, and filter with a microporous membrane;

[0028] 2) Take the homogenized aquatic product and put it in a centrifuge tube, add the lomefloxacin solution prepared in step 1), the solid-liquid ratio is 1g:150mL, add anhydrous copper sulfate and monoethanolamine after shaking, and centrifuge at 12000rpm for 15min, Take the supernatant, dissolve the residue with monoethanolamine and centrifuge, and combine the supernatant for later use; the amount of anhydrous copper sulfate added is 3 times the quality of the aquatic product, and the amount of monoethanolamine added is 20 mL for 1 g of aquatic product;

[0029] 3) Add n-hexane solution of 3 times the volume of the supernatant to the supernatant in step 2), d...

Embodiment 2

[0033] A method for detecting lomefloxacin enantiomers in aquatic products, comprising the following steps:

[0034] 1) Use a mixture of isopropanol and n-heptane with a volume ratio of 90%: 10% as the mobile phase to dissolve and dilute lomefloxacin to a solution of 40 μg / mL, and filter it with a microporous membrane;

[0035] 2) Take the homogenized aquatic product and place it in a centrifuge tube, add the lomefloxacin solution prepared in step 1), the solid-liquid ratio is 1g:120mL, add anhydrous copper sulfate and monoethanolamine after shaking, and centrifuge at 8000rpm for 10min, Take the supernatant, dissolve the residue with monoethanolamine and centrifuge, and combine the supernatant for later use; the amount of anhydrous copper sulfate added is 3 times the quality of the aquatic product, and the amount of monoethanolamine added is 20 mL for 1 g of aquatic product;

[0036] 3) Add a n-hexane solution twice the volume of the supernatant to the supernatant in step 2), ...

Embodiment 3

[0040] A method for detecting lomefloxacin enantiomers in aquatic products, comprising the following steps:

[0041] 1) Use a mixture of isopropanol and n-heptane with a volume ratio of 90%:10% as the mobile phase to dissolve and dilute lomefloxacin to a solution of 45 μg / mL, and filter it with a microporous membrane;

[0042] 2) Take the homogenized aquatic product and put it in a centrifuge tube, add the lomefloxacin solution prepared in step 1), the solid-liquid ratio is 1g:130mL, add anhydrous copper sulfate and monoethanolamine after shaking, and centrifuge at 9000rpm for 12min, Take the supernatant, dissolve the residue with monoethanolamine and centrifuge, and combine the supernatant for later use; the amount of anhydrous copper sulfate added is 3 times the quality of the aquatic product, and the amount of monoethanolamine added is 20 mL for 1 g of aquatic product;

[0043] 3) Add n-hexane solution of 3 times the volume of the supernatant to the supernatant in step 2), ...

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Abstract

The invention relates to a method for detecting a lomefloxacin enantiomer in an aquatic product. According to the present invention, the solid-phase extraction condition is optimized, such that the solid-phase extraction time is shortened, and the efficiency is high; the high performance liquid chromatography method for analyzing the lomefloxacin enantiomer concentration has characteristics of high precision, rapidness, simple operation, and low cost; and the detection method has characteristics of good linearity to the target compound, high recovery rate, low relative deviation, low toxicity of the used reagent, low consumption of the used reagent, and environmental protection.

Description

technical field [0001] The invention relates to a method for rapidly and efficiently detecting lomefloxacin enantiomers in aquatic products. Background technique [0002] Lomefloxacin is a quinolone antibiotic. It has high antibacterial activity against Enterobacteriaceae bacteria such as Escherichia coli, Shigella, Klebsiella, Proteus, Enterobacter, etc.; Haemophilus influenzae, Neisseria gonorrhoeae, etc. It is highly sensitive; it also has certain antibacterial effects on Acinetobacter, Pseudomonas aeruginosa and other Pseudomonas, Staphylococcus, pneumococcus, and hemolytic streptococcus. This product plays a bactericidal role by acting on the A subunit of DNA helicase in bacterial cells, inhibiting DNA synthesis and replication. It is currently used in aquaculture. [0003] At present, the commonly used separation methods of enantiomers of chiral drugs include crystallization, kinetic methods, enzymatic resolution and chromatography, etc. These methods are widely use...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 杨立业欧阳小琨王阳光金如娜王艳飞卢娇
Owner ZHEJIANG OCEAN UNIV
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