Timosaponin AIII nano liposome as well as preparation method and application thereof
The technology of nanoliposome and timosaponin is applied in the field of preparation of medicine and traditional Chinese medicine. Easy to obtain, improve absorption in the body, prolong the effect of drug action time
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Embodiment 1
[0038] Embodiment 1, timosaponin AⅢ nano liposome preparation (prescription 1)
[0039]Weigh 0.8 mg timosaponin AⅢ and dissolve in 400 μL methanol; weigh 8.35 mg egg yolk lecithin (EPC) and dissolve it in 200 μL chloroform solution, and ultrasonically dissolve. Combine the above solutions into a round-bottomed test tube with a stopper, blow dry with nitrogen to remove methanol and chloroform, until a uniform lipid film is formed on the wall of the round-bottomed test tube. The molar ratio of EPC to timosaponin AⅢ was 10:1. -0.09MPa, 45~50℃, dry under reduced pressure to remove residual solvent and moisture, add 200μL 0.01mol / L phosphate buffer (pH=7.4), hydrate under 45~50℃ water bath for 1 hour, and obtain timosaponin AⅢ ester Plastid suspension. The timosaponin AⅢ liposome suspension is sonicated 3 times under the conditions of ultrasonic frequency 40-100 Hz and power 200-300 W, each ultrasonic time is 3-5 min, to obtain timosaponin AⅢ nano liposome.
Embodiment 2
[0040] Embodiment 2, timosaponin AⅢ nano liposome preparation (prescription 2)
[0041] Weigh 0.8 mg timosaponin AⅢ and dissolve in 400 μL methanol, weigh 8.53 mg distearoylphosphatidylcholine (DSPC) and dissolve in 200 μL chloroform solution, and dissolve by ultrasonication. Combine the above solutions into a round-bottomed test tube with a stopper, blow dry with nitrogen to remove methanol and chloroform, until a uniform lipid film is formed on the wall of the round-bottomed test tube. The molar ratio of DSPC to timosaponin AⅢ was 10:1. -0.09MPa, 45~50℃, dry under reduced pressure to remove residual solvent and moisture, add 200μL 0.01mol / L phosphate buffer (pH=7.4), hydrate under 45-50℃ water bath for 1 hour, and obtain timosaponin AⅢ ester Plastid suspension. The timosaponin AⅢ liposome suspension is subjected to ultrasonication at an ultrasonic frequency of 40-100 Hz and a power of 200-300 W, and ultrasonicated 3 times for 3-5 minutes each time to obtain timosaponin AⅢ ...
Embodiment 3
[0042] Embodiment 3, timosaponin AⅢ nano liposome preparation (prescription 3)
[0043] Weigh 0.8 mg timosaponin AⅢ and dissolve in 400 μL methanol, weigh 7.51 mg egg yolk lecithin (EPC), 3.02 mg distearoylphosphatidylethanolamine-polyethylene glycol 2000 (DSPE-PEG2000) and dissolve in 500 μL chloroform solution , ultrasonically dissolved, combined the above solutions in a round-bottomed test tube, blown dry with nitrogen to remove methanol and chloroform, until a uniform lipid film was formed on the wall of the round-bottomed test tube. The molar ratio of EPC, DSPE-PEG2000 and timosaponin AⅢ was 9:1:1. -0.09MPa, 45~50℃, dry under reduced pressure to remove residual solvent and moisture, add 200μL 0.01mol / L phosphate buffer (pH=7.4), hydrate under 45-50℃ water bath for 1 hour, and obtain timosaponin AⅢ ester Plastid suspension. The timosaponin AⅢ liposome suspension is subjected to ultrasonication at an ultrasonic frequency of 40-100 Hz and a power of 200-300 W, and ultrason...
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