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Composite material modified electrode used for measuring glucose concentration and application thereof

A technology of modifying electrodes and composite materials, which is applied in the field of composite materials modifying electrodes, can solve the problems of insufficient electrode stability, large temperature influence, and narrow measurement range, and achieve stable measurement, good catalytic activity, and improved electrode stability.

Active Publication Date: 2016-11-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many methods for measuring glucose, among which the biosensor based on glucose oxidase has disadvantages such as easy inactivation of immobilized enzyme, great influence of temperature, and narrow measurement range. Therefore, an enzyme-free electrochemical sensor is established to measure glucose. of great significance
Electrochemical analysis methods have been widely used due to the advantages of convenient detection, high sensitivity, good selectivity, and low cost. The research mainly focuses on metal or metal oxide modified electrodes, but these electrodes are sometimes not stable enough, so the preparation of a New modified electrodes appear urgent

Method used

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  • Composite material modified electrode used for measuring glucose concentration and application thereof
  • Composite material modified electrode used for measuring glucose concentration and application thereof
  • Composite material modified electrode used for measuring glucose concentration and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Preparation of polyaspartic acid / platinum nanocomposite modified electrode (PASP / Pt / GCE) for glucose determination, comprising the following steps:

[0025] (1) Glassy carbon electrode pretreatment: Use 300nm and 50nm α-Al on the suede in sequence 2 o 3 Polish the glassy carbon electrode to the mirror surface with the paste polishing solution, then use absolute ethanol and deionized water to ultrasonically wash the electrode for 1min, and then place the cleaned glassy carbon electrode in 0.5mol / L dilute sulfuric acid solution, in- The 0.5-1.2V potential range was activated by cyclic voltammetry at a scan rate of 100mV / s until the response was stable. Finally, the glassy carbon electrode was rinsed with deionized water and dried for later use.

[0026] (2) Before modifying the glassy carbon electrode, the electrode needs to be at 1mmol / L K 3 [Fe(CN) 6 ] solution (containing 0.1mol / L KCl) to conduct cyclic voltammetry scanning to verify the reversibility of the electro...

Embodiment 2

[0036] Preparation of polyaspartic acid / platinum nanocomposite modified electrode (PASP / Pt / GCE) for glucose determination, comprising the following steps:

[0037] (1) Glassy carbon electrode pretreatment: Use 300nm and 50nm α-Al on the suede in sequence 2 o 3 Polish the glassy carbon electrode to the mirror surface with paste polishing liquid, then apply absolute ethanol and deionized water to ultrasonically wash the electrode for 90s, and then place the cleaned glassy carbon electrode in 0.4mol / L dilute sulfuric acid solution, in- In the 0.6-1.4V potential range, cyclic voltammetry activation was performed at a scan rate of 120mV / s until the response was stable. Finally, the glassy carbon electrode was rinsed with deionized water and dried for later use.

[0038] (2) The pretreated glassy carbon electrode was placed in a solution of 1.5mmol / L aspartic acid (a phosphate buffer solution with a concentration of 0.15mol / L and a pH value of 6.5), and electrodeposition was perfor...

Embodiment 3

[0042] Preparation of polyaspartic acid / platinum nanocomposite modified electrode (PASP / Pt / GCE) for glucose determination, including the following steps

[0043] (1) Glassy carbon electrode pretreatment: Use 300nm and 50nm α-Al on the suede in sequence 2 o 3 Polish the glassy carbon electrode to the mirror surface with the paste polishing solution, then use absolute ethanol and deionized water to ultrasonically wash the electrode for 2 minutes, and then place the cleaned glassy carbon electrode in 0.6mol / L dilute sulfuric acid solution, in- The 0.8-1.5V potential range was activated by cyclic voltammetry at a scan rate of 140mV / s until the response was stable. Finally, the glassy carbon electrode was rinsed with deionized water and dried for later use.

[0044] (2) The pretreated glassy carbon electrode was placed in a solution of 3 mmol / L aspartic acid (a phosphate buffer solution with a concentration of 0.2 mol / L and a pH value of 7), and electrodeposition was performed by ...

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Abstract

The invention discloses a composite material modified electrode used for measuring glucose concentration and application thereof. According to the invention, firstly a polyamino acid modified electrode is prepared by virtue of a cyclic voltammetry, then the cyclic voltammetry is adopted for depositing metal nanoparticles on the surface of the polyamino acid film modified electrode, and a polyamino acid / metal nanocomposite material modified electrode is prepared. The obtained modified electrode is taken as a working electrode, a silver / silver chloride electrode is taken as a reference electrode, a platinum cylinder electrode is taken as an auxiliary electrode, and an amperometry is applied in a three-electrode system for realizing linear electrochemical response of glucose in a certain concentration range. The modified electrode has the characteristics of sensitive response and good stability.

Description

technical field [0001] The invention belongs to the technical field of food analysis and detection, and in particular relates to a composite material modified electrode for measuring glucose concentration and its application. Background technique [0002] Glucose is the most widely distributed and most important monosaccharide in nature. It plays an important role in the field of biology. It is the energy source and metabolic intermediate product of living cells. Most of the energy required for human activities is obtained from sugar in food intake. These sugars are finally broken down into glucose to provide energy. [0003] There are many methods for measuring glucose, among which the biosensor based on glucose oxidase has disadvantages such as easy inactivation of immobilized enzyme, great influence of temperature, and narrow measurement range. Therefore, an enzyme-free electrochemical sensor is established to measure glucose. is of great significance. Electrochemical a...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3277
Inventor 韦真博张伟林王俊王永维
Owner ZHEJIANG UNIV
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