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Calcium carbonate/hyaluronic acid compound medicine carrier and preparing method thereof

A technology of hyaluronic acid and calcium carbonate, which is applied in drug combinations, pharmaceutical formulations, peptide/protein components, etc., can solve the problems of complex structure and unreliable insulin loading, and achieve the advantages of simple preparation method, improved drug efficacy, and reduced trauma. Effect

Active Publication Date: 2016-11-23
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The drug carrier has shortcomings such as complex structure and unreliable insulin loading.

Method used

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  • Calcium carbonate/hyaluronic acid compound medicine carrier and preparing method thereof
  • Calcium carbonate/hyaluronic acid compound medicine carrier and preparing method thereof
  • Calcium carbonate/hyaluronic acid compound medicine carrier and preparing method thereof

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Embodiment 1

[0027] The preparation method of the calcium carbonate / hyaluronic acid composite insulin drug carrier of the present embodiment comprises the following steps:

[0028] (1) Sodium carbonate is dissolved in deionized water, and preparation obtains the sodium carbonate solution that carbonate ion concentration is 0.1mol / L; Calcium chloride is dissolved in deionized water, and preparation obtains calcium ion concentration and is the chlorine of 0.12mol / L Calcium solution, ready for use after preparation;

[0029] (2) dissolving folic acid-modified hyaluronic acid in deionized water to prepare a hyaluronic acid solution with a mass percentage of 1%, and prepare it for later use;

[0030] (3) dissolving insulin in a buffered aqueous solution with pH=2.0, preparing an insulin solution with a concentration of 0.01mol / L, and then adjusting the pH of the insulin solution to 8.0 through the buffer;

[0031] (4) Prepare the oil phase containing surfactant, disperse surfactant sorbitan fa...

Embodiment 2

[0037] The preparation method of the calcium carbonate / hyaluronic acid composite insulin drug carrier of the present embodiment comprises the following steps:

[0038](1) Sodium carbonate is dissolved in deionized water, and preparation obtains the sodium carbonate solution that carbonate ion concentration is 0.1mol / L; Calcium chloride is dissolved in deionized water, and preparation obtains calcium ion concentration and is the chlorine of 0.12mol / L Calcium solution, ready for use after preparation;

[0039] (2) Dissolving folic acid-modified hyaluronic acid in deionized water to prepare a 2% hyaluronic acid solution by mass, which is prepared for later use;

[0040] (3) dissolving insulin in a buffered aqueous solution with pH=2.0, preparing an insulin solution with a concentration of 0.01mol / L, and then adjusting the pH of the insulin solution to 8.0 through the buffer;

[0041] (4) Prepare the oil phase containing surfactant, disperse surfactant sorbitan fatty acid ester (...

Embodiment 3

[0046] The calcium carbonate / hyaluronic acid compound insulin drug carrier that embodiment 1, embodiment 2 make, simple insulin, insulin are loaded on the calcium carbonate nanoparticle and carry out the simulation experiment in the gastric juice, the insulin in the above four kinds of materials is equivalent , the loading amount of insulin is 0.3μg, the four kinds of materials are mixed with 3mL of simulated gastric acid solution with pH=1.2, the simulated gastric acid solution contains pepsin with a mass concentration of 3g / L, after incubation at 37°C for 30min, 0.2mL of 0.1mol / L NaOH terminates the activity of pepsin. Figure 7 It shows the retention effect of insulin against pepsin under different conditions. Pure insulin is completely dissolved by pepsin. Insulin loaded on calcium carbonate nanoparticles can improve the anti-pepsin effect; calcium carbonate nanocarriers are coated with hyaluronic acid It can delay the destruction of the carrier and drug molecules by diges...

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Abstract

The invention belongs to the technical field of medicine carrier materials, and particularly relates to a calcium carbonate / hyaluronic acid compound medicine carrier. The calcium carbonate / hyaluronic acid compound medicine carrier comprises a core and a coating layer, the core is prepared from porous nanometer calcium-carbonate particles for loading insulin medicine, and the coating layer is prepared from hyaluronic acid modified through folic acid. The invention further discloses a preparing method of the calcium carbonate / hyaluronic acid compound medicine carrier, and the preparing method includes the following steps that 1, at the room temperature, insulin medicine is dissolved in water, then an insulin medicine water solution is dispersed into an oil phase containing a surfactant, and W / O type emulsion is prepared; 2, a water-soluble calcium salt solution and a water-soluble carbonate solution are prepared; 3, a calcium-ion water solution and a carbonate-ion water solution are slowly dropped into the W / O type emulsion respectively, and trace and overmuch calcium ions are controlled; 4, a hyaluronic acid water solution is prepared and slowly dropped into the emulsion prepared in the step 3, the mixture is stirred and subjected to centrifugal separation, and a compound medicine carrier is obtained. The preparing method is simple, and scale production can be achieved.

Description

technical field [0001] The invention belongs to the technical field of drug carrier materials, and in particular relates to a calcium carbonate / hyaluronic acid composite drug carrier and a preparation method thereof. Background technique [0002] Diabetes (Diabetes) is a public health problem worldwide, which seriously threatens human health and life. In 2015, there were about 415 million adults in the world suffering from diabetes to varying degrees, and it is still growing rapidly. The number of adult diabetic patients in China has reached 109 million, accounting for 26.3% of the global diabetic patients. Diabetes is a chronic disease in which glucose in the blood tends to accumulate too much. Clinically, it is characterized by "hyperglycemia". Absolutely lacking, the most direct and effective route of administration for the control of blood sugar levels in diabetics is subcutaneous or intravenous insulin. But there are many deficiencies in this way of administration, su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/02A61K47/36A61K38/28A61P3/10
CPCA61K38/28A61K47/02A61K9/5036A61K9/5078
Inventor 江国华刘德朋孔祥东姚菊明童再再
Owner ZHEJIANG SCI-TECH UNIV