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A method for producing Bacillus subtilis by solid fermentation using chondroitin sulfate to produce industrial waste bone sludge as raw material

A technology of Bacillus subtilis and chondroitin sulfate, which is applied in the field of microorganisms to achieve the effects of easy operation, lower price, and convenience for industrialized large-scale production and application

Inactive Publication Date: 2019-04-05
香河县龙津生物工程有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the production of Bacillus subtilis by fermentation of waste bone mud

Method used

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  • A method for producing Bacillus subtilis by solid fermentation using chondroitin sulfate to produce industrial waste bone sludge as raw material
  • A method for producing Bacillus subtilis by solid fermentation using chondroitin sulfate to produce industrial waste bone sludge as raw material
  • A method for producing Bacillus subtilis by solid fermentation using chondroitin sulfate to produce industrial waste bone sludge as raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Prepare 100 kg of solid fermentation medium according to the mass ratio of waste bone mud, bran and water 3:20:60, stir evenly, sterilize at 120°C for 20 minutes, press 5×10 5 Bacillus subtilis was inserted into the culture medium per g, solid fermentation at 35-37°C for 48 hours, the fermentation process was divided into two stages: static culture was adopted for 1-8 hours, after 8 hours, the temperature was controlled by sterile air at 35-37°C, and stirring was started every 6 hours 20rpm time 10min. After 48 hours of fermentation, the fermentation product was collected. Dried at 50-60°C until the water content is lower than 8%, crushed and passed through an 80-mesh sieve to obtain Bacillus subtilis for feed. According to GB / T 26428-2010 "Detection of Bacillus subtilis in microbial preparations for feed" method to detect the number of Bacillus subtilis >6.0×10 10 pcs / g.

Embodiment 2

[0028] Example 2: Prepare 100 kg of solid fermentation medium according to the mass ratio of waste bone mud, bran and water 8:30:80, stir evenly, sterilize at 120°C for 20 minutes, press 1×10 6 Bacillus subtilis was inserted into the culture medium per g, and solid fermentation was carried out at 35-37°C for 60 hours. The fermentation process is divided into two stages: static culture is adopted for 1-8h, and after 8h, the temperature is controlled by sterile air at 35-37°C, and stirring is started at 20rpm for 10min every 6h. After 60 h of fermentation, the fermentation product was collected. Dried at 50-60°C until the water content is lower than 8%, crushed and passed through an 80-mesh sieve to obtain Bacillus subtilis for feed. According to GB / T 26428-2010 "Detection of Bacillus subtilis in feed microbial preparations" method to detect the number of Bacillus subtilis > 1.0×10 11 pcs / g.

Embodiment 3

[0029] Example 3: Prepare 100 kg of solid fermentation medium according to the mass ratio of waste bone mud, bran, and water 3:10:20. After stirring evenly, sterilize at 120° C. for 20 minutes, and press 5×10 6 Bacillus subtilis was inserted into the culture medium per g, and solid fermentation was carried out at 35-37°C for 72 hours. The fermentation process is divided into two stages: static culture is adopted for 1-8h, and after 8h, the temperature is controlled by sterile air at 35-37°C, and stirring is started at 20rpm for 10min every 6h. After 72 hours of fermentation, the fermentation products were collected. Dried at 50-60°C until the water content is lower than 8%, crushed and passed through an 80-mesh sieve to obtain Bacillus subtilis for feed. According to GB / T 26428-2010 "Detection of Bacillus subtilis in microbial preparations for feed" method to detect the number of Bacillus subtilis >8×10 10 pcs / g.

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Abstract

The invention provides a method for producing bacillus subtilis through solid fermentation and by taking waste bone paste as a raw material in the chondroitin sulfate production industry. The method comprises the following steps: with the industrial waste bone paste generated by the technology of extracting chondroitin sulfate from animal cartilage as a matrix, mixing the matrix with bran and water to obtain a medium; performing high-temperature sterilization; inoculating bacillus subtilis and performing solid fermentation; and drying and grinding the fermented product to obtain bacillus subtilis. In the method provided by the invention, the bacillus subtilis with high added value can be produced by effectively utilizing low-cost leftover waste bone paste to achieve an aim of turning waste into wealth; and moreover, the technology is simple, the cost is low, and the operation is easy. In the invention, the waste bone paste of the chondroitin sulfate production technology is applied to the fermentation production of bacillus subtilis for the first time, and a new way is opened up for producing industrial waste bone paste from chondroitin sulfate.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for producing Bacillus subtilis by solid fermentation using chondroitin sulfate production industrial waste bone mud as a raw material. Background technique [0002] Chondroitin sulfate exists in the form of proteoglycan in cartilage tissue. The polysaccharide chain of chondroitin sulfate in proteoglycan is connected to the core protein by covalent bond (glycopeptide bond), while the aggregate of proteoglycan is connected by secondary bond (hydrogen bond). and salt bonds) are formed. It can be seen that the extraction of chondroitin sulfate from animal cartilage tissue must take appropriate and effective methods to destroy the covalent bond between polysaccharide chains and proteins, so that polysaccharide chains and proteins can be effectively separated, so that polysaccharide chondroitin sulfate can be extracted efficiently. At present, domestic man...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/125
CPCC12N1/20
Inventor 姜龙祥石苑婷吕伟杨
Owner 香河县龙津生物工程有限责任公司
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