Rapid detection method for researching human azoospermia and severe oligozoospermia and kit using method

A technique for azoospermia and oligospermia, which is applied in the field of detection and research of human sperm, can solve the problems of low efficiency and long detection time, and achieve the effect of high efficiency

Inactive Publication Date: 2016-11-23
JILIN UNIV
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Problems solved by technology

[0004] The existing clinical detection methods for azoospermia and severe oligospermia mainly include karyotype analysis and AZF microdeletion detection. %) causes of spermatogenic disorders

Method used

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Embodiment Construction

[0019] The present invention proposes a method for rapid testing of human azoospermia and severe oligozoospermia. The steps include: 1. Sample collection: extract the same equal parts of blood and sperm from patients with severe oligospermia / azospermia and normal men slurry, urine samples, and extract the testicular tissue and liquid nitrogen in the same aliquots of patients with severe oligospermia / azoospermia and normal male testes for preservation. In this embodiment, the collection of testicular tissue is carried out by using line 2. Sample processing, including: seminal plasma processing, urine processing, and testicular tissue processing; seminal plasma processing refers to the difference between centrifuging the semen, collecting the supernatant, and extracting the seminal plasma protein, and finally use the western blotting method to verify the differential protein. In some embodiments, protein labeling means that the relative expression of the protein is expressed by t...

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Abstract

The invention relates to research of a human azoospermia and severe oligozoospermia detection method, and provides a rapid detection method for researching human azoospermia and severe oligozoospermia. The method includes the steps: acquiring samples; treating the samples; performing diagnosis by the aid of a real-time PCR (real-time polymerase chain reaction) platform; performing ELISA (enzyme-linked immuno sorbent assay) detection by the aid of sample treating results. The invention further provides a kit using the method. The kit comprises RNAs (ribose nucleic acid) and tissue metabolites, wherein the RNAs are extracted after the samples are treated. The research method helps patients suffering from the human azoospermia and the severe oligozoospermia to be rapidly detected and diagnosed, and the kit can help the patients to rapidly find pathogenesis for treatment.

Description

technical field [0001] The invention relates to the detection and research field of human sperm, in particular to the research on the detection method of human azoospermia and severe oligospermia and a kit for applying the method. Background technique [0002] According to statistics, more than 25% of couples of childbearing age are unable to conceive within one year, and about 50% of them are caused by male factors. Spermia and severe oligospermia. The etiology of male spermatogenic disorder is being paid more and more attention. However, due to the complexity of the spermatogenic process, the pathophysiological understanding of spermatogenesis has been insufficient for a long time, which leads to difficulties in the diagnosis, treatment and prognosis of these patients. [0003] At present, the etiology of spermatogenic disorder in most patients with azoospermia and severe oligozoospermia is unknown, which brings great confusion to clinical treatment. Specific markers for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68G01N33/53
CPCC12Q1/6883C12Q2600/158C12Q2600/178G01N33/5308G01N33/6893G01N2800/34
Inventor 郑连文徐影代晓微陈晓晨郑晓宇刘永正
Owner JILIN UNIV
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