A kind of detection method of andrographolide content in andrographis preparations

Abstract

The invention discloses a method for detecting the content of andrographolide in a common andrographis herb preparation. The method includes the following steps that 1, an andrographolide reference substance is taken and dissolved in a solvent to serve as a reference substance solution; 2, a test sample is taken and dissolved in a solvent to serve as a test sample solution; 3, differential pulse voltammetry electrochemical detection is carried out on the test sample solution and the reference substance solution, wherein in electrochemical detection, a supporting electrolyte is a phosphate buffered solution, a working electrode is a nanometer copper oxide / Nafion modified gold electrode, a reference electrode is a saturated calomel electrode, and an auxiliary electrode is a platinum wire electrode; 4, a standard curve is drawn, and the content of andrographolide in the common andrographis herb preparation is calculated. The method is simple, rapid and economical and can be used for rapidly detecting andrographolide on the scene.

Description

technical field [0001] The invention relates to the detection of medicines, in particular to a detection method for andrographolide content in andrographolide preparations. Background technique [0002] Andrographolide is the main active ingredient of Andrographis paniculata (Burm.f.) Nees, a plant of the Acanthaceae family. The structure is as follows: [0003] [0004] Modern pharmacological studies have shown that andrographolide and its derivatives have anti-pathogenic microorganisms, antipyretic, anti-inflammatory, anti-fertility, anti-cardiovascular disease, immune stimulation, gallbladder and liver protection, anti-tumor effects [1], and anti-bacterial Sexual and viral upper respiratory tract infections and dysentery have special effects [2], known as natural antibiotics. It is clinically used for meningitis, bacillary dysentery, pneumonia, leptospirosis, enhancing adrenal cortex function, upper respiratory tract infection, etc. [3]. [0005] At present, the meth...

AI Technical Summary

Problems solved by technology

However, the above-mentioned methods or experimental conditions are harsh, or the operation steps are cumbersome, or the required instruments are expensive and time-consuming, so they are not suitable for large-scale promotion and application, nor are they suitable for ready-made rapid detection.

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