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Construction method of fingerprints of rhizoma corydalis analgesic capsules and application of construction method

A kind of Yuanhu Zhitong, fingerprint technology, applied in the establishment of Yuanhu Zhitong Capsule HPLC fingerprint, Yuanhu Zhitong Capsule fingerprint field, can solve the influence of formula preparation quality control and evaluation, limited liquid chromatography separation ability, Authenticity restrictions and other issues, to achieve the effect of targeted and practical quality control

Active Publication Date: 2016-11-23
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex components contained in the medicinal materials of the prescription, and the limited separation ability of liquid chromatography, the components interfere with each other, resulting in serious overlapping of some chromatographic peaks in the fingerprints obtained under the same sample preparation method and the same chromatographic conditions, it is difficult to tell clearly The origin of the medicinal material components of the chromatographic peak (that is, which medicinal material it comes from)
The fingerprint chromatogram established in this way will be subject to certain restrictions on the quality control and evaluation of a certain group of medicinal materials, and the authenticity of the inspection preparation feeding, and will also have a certain impact on the quality control and evaluation of the entire prescription preparation.

Method used

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  • Construction method of fingerprints of rhizoma corydalis analgesic capsules and application of construction method
  • Construction method of fingerprints of rhizoma corydalis analgesic capsules and application of construction method
  • Construction method of fingerprints of rhizoma corydalis analgesic capsules and application of construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1: Construction of Yuanhu Zhitong Capsule Fingerprints Reflecting the Fingerprint Characteristics of Corydalis Corydalis and Methodology Verification

[0064] Sample information: commercially available drugs, see Table 1 for sample information.

[0065] Table 1:

[0066]

[0067] The specific steps are:

[0068] (1) Preparation of test solution: take Yuanhu Zhitong Capsule, pour out the contents, add ammonia water to infiltrate, seal for 15min, use methanol as solvent, the ratio of Yuanhu Zhitong Capsule to methanol addition is 1g:12ml, ultrasonic frequency is 30KHz, and the ultrasonic time is 30min. Perform ultrasonic extraction, filter, evaporate the filtrate to dryness, dissolve the residue in water, transfer it to a separatory funnel, adjust the pH to 2-3, extract 2 times with petroleum ether (60-90°C), 40 mL each time, and discard the petroleum ether layer , the aqueous layer was adjusted to pH 9-10 by adding concentrated ammonia test solution, ext...

Embodiment 2

[0098] Embodiment 2: The construction of Yuanhu Zhitong Capsule fingerprints reflecting the fingerprint characteristics of Angelica Radix and methodological verification

[0099] The sample information is the same as Table 1 in Example 1.

[0100] The specific steps are:

[0101] (1) Preparation of test solution: take Yuanhu Zhitong capsule, pour out the contents, grind finely, pass through a No. 6 sieve, take 1 g, accurately weigh it, place it in a Soxhlet extractor, add methyl tert-butyl ether 15mL, reflux extraction for 6 hours, allow to cool, recover the ether solution, dissolve the residue in methanol and transfer it to a 25mL volumetric flask, add methanol to the mark, shake well, filter, take the subsequent filtrate and filter it with a 0.22μm microporous membrane , as the test solution.

[0102] (2) Preparation of reference medicinal material solution: take 1 g of Angelica dahurica reference medicinal material, and prepare a reference medicinal material solution acco...

Embodiment 3

[0131] Example 3: Using the established Yuanhu Zhitong Capsule fingerprints to evaluate the quality of samples from different manufacturers and batches

[0132] The quality control of Yuanhu Zhitong Capsules is carried out by using fingerprints, and the specific method includes the following steps:

[0133] (1) Construction of the fingerprint of the Yuanhu Zhitong Capsule sample to be tested: take 10 batches of the Yuanhu Zhitong Capsule sample to be tested, and construct the fingerprint of the Yuanhu Zhitong Capsule sample to be tested by the methods of Example 1 and Example 2;

[0134] (2) The HPLC fingerprint chromatographic peaks of 10 batches of Yuanhu Zhitong Capsule samples were matched by the software of "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System". Select S1 (sample number 1) as the reference spectrum, and the generated control fingerprint is R. See Table 14 and Table 15 for the fingerprints of each batch of samples and the control fing...

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Abstract

The invention discloses a construction method of fingerprints of rhizoma corydalis analgesic capsules, aiming at construction of fingerprints of components of the rhizoma corydalis analgesic capsules, namely rhizoma corydalis and angelica dahurica. The construction method includes preparing test sample solutions, preparing reference substance solutions, preparing reference herb solutions, preparing negative reference solutions, performing liquid phase measurement, and processing the obtained fingerprints by fingerprint software to obtain the fingerprints representing fingerprint characteristics of the rhizoma corydalis and the fingerprints representing fingerprint characteristics of the angelica dahurica respectively. By the constructed fingerprints of the rhizoma corydalis analgesic capsules, mutual interference among medicinal elements can be avoided effectively, authenticity and quality of herbs applied to preparations can be tested conveniently, quality of the rhizoma corydalis analgesic capsules can be evaluated accurately from part to whole, and quality standards and control methods of the rhizoma corydalis analgesic capsules can be improved and perfected.

Description

technical field [0001] The invention relates to a method for analyzing fingerprints of traditional Chinese medicine preparations, in particular to a method for establishing HPLC fingerprints of Yuanhuzhitong capsules and the fingerprints of Yuanhuzhitong capsules obtained by the method, which are used for samples of Yuanhuzhitong capsules from different manufacturers and different batches. quality evaluation. Background technique [0002] The fingerprint of traditional Chinese medicine refers to the chromatogram (or spectrogram) that can identify the characteristics of a Chinese medicinal material or a Chinese patent medicine after proper processing and certain analytical methods. The chromatographic method is the mainstream method. The characteristics of traditional Chinese medicine fingerprints can effectively identify the authenticity of samples through the fingerprint characteristics of the fingerprints; secondly, through the area and proportion of its main characterist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 姜红冯凯聂磊
Owner SHANDONG UNIV