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Preparation method for high-porosity high-connectivity biological scaffold
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A high-porosity, bio-stent technology, applied in drug delivery, pharmaceutical formulations, coatings, etc., can solve the problems of cell interconnection limitations, inability to process three-dimensional scaffolds, poor hydrophilicity and biocompatibility, etc., to achieve The effect of repeatability and flexibility of production process, improvement of hydrophilicity and biocompatibility, and simple operation process
Active Publication Date: 2016-12-07
FUJIAN UNIV OF TECH
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Problems solved by technology
However, there is still no technology that can produce tissue engineering scaffolds that fully meet the needs of cells and tissues.
For solution casting / particle leaching technology, bioscaffolds with higher porosity can be prepared, but the inter-connectivity of cells inside the scaffold is poor; thermally induced phase separation technology can form a variety of microscopic pore structures, and It has a high porosity, but the micropores generally prepared have a small pore size, and the interconnectivity of the cells is often limited, which affects the entry and proliferation of cells; extrusion molding technology cannot process three-dimensional scaffolds with complex shapes; and injection molding Although the particle leaching method has the advantages of low processing cost, repeatability of the production process, and flexibility in the shape design of the product, it greatly increases the processing difficulty while increasing the porosity of the bracket, so it is also difficult to further develop. Improve the porosity and connectivity of the scaffold; injection molding / supercritical fluid technology can effectively increase the porosity of the scaffold and reduce the difficulty of processing, but for some materials, such as crystalline polymers, the foam structure produced by this method is mostly closed. Pore structure, so it does not significantly improve the connectivity of cells inside the scaffold
In addition, most of the synthetic polymer bioscaffolds with various structures currently prepared have the disadvantages of poor hydrophilicity and biocompatibility.
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Embodiment 1
[0046] A preparation method based on PLA biological support, comprising the following preparation steps:
[0047] (1) Dissolve 3.2, 3.6, and 4 g of PLA in 20 ml of chloroform, respectively, and shake for 6 hours to obtain 16, 18, and 20% (w / v) PLA homogeneous solutions.
[0048] (2) Use a standard sieve to sieve the NaCl particles into 150-212, 212-300, 300-425 μm grades.
[0049] (3) Weigh 40g of NaCl particles in the size range screened in the above step 2, place them in a metal mold of 15cm×10cm×5cm, and press them under a pressure of 16kPa for 10min with a hot press to prepare a pore-forming agent with a dense structure Preform.
[0050] (4) Firstly, the pore-forming agent preform prepared in step 3 is placed on a flat operating table, and then a release cloth is laid up and down the preform, and the guide net is placed on the upper release cloth, Finally, vacuum bagging is used to seal the above preform area. There are two rubber hose inlets and outlets in the sealed a...
Embodiment 2
[0062] A preparation method based on PCL bioscaffold, comprising the following preparation steps:
[0063] (1) Dissolve 4.8, 5.6, and 6.4 g of PCL in 20 ml of chloroform, respectively, and shake for 6 hours to obtain uniform solutions of 24, 28, and 32% (w / v) PCL.
[0064] (2) Use a standard sieve to sieve the NaCl particles into 150-300, 300-450, 450-600 μm grades.
[0065] (3) Weigh 40g of NaCl particles in the size range screened in the above step 2, place them in a metal mold of 15cm×10cm×5cm, and press them under a pressure of 16kPa for 10min with a hot press to prepare a pore-forming agent with a dense structure Preform.
[0066] (4) Firstly, the pore-forming agent preform prepared in step 3 is placed on a flat operating table, and then a release cloth is laid up and down the preform, and the guide net is placed on the upper release cloth, Finally, vacuum bagging is used to seal the above preform area. There are two rubber hose inlets and outlets in the sealed area, o...
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Abstract
The invention discloses a preparation method for a high-porosity high-connectivity biological scaffold. A three-dimensional porous scaffold prepared according to the method provided by the invention has higher porosity, larger aperture and excellent connectivity and is favorable for cell entering, proliferation, conveying and discharging of nutrient solution and metabolin. The aperture of the scaffold is adjustable and can form a bubble form with a porous structure. Besides, the biological scaffold prepared by adopting natural chitosan is subjected to surface coating modification, so that the hydrophilic performance of the scaffold is greatly increased and the planting and growth of the cells are benefited. The invention also aims to provide the preparation method for the biological scaffold. The preparation method has the advantages of low processing cost, production technology repeatability, flexibility of product shape design, and the like. The preparation method is an ideal method for processing and manufacturing the three-dimensional porous scaffold.
Description
technical field [0001] The invention belongs to the technical field of polymer material processing, and in particular relates to a method for preparing a biological scaffold with high porosity and high connectivity. Background technique: [0002] Tissue engineering is an emerging interdisciplinary subject, which integrates the basic principles, basic theories, basic technologies and basic methods of life science, engineering and materials science. Tissue engineering mainly covers the following three parts: cells, scaffolds and growth information. Among them, cells are the most basic structural units of all biological tissues, and tissue engineering scaffolds are used as a place for planting cells and a template for tissue regeneration, and its internal microporous structure and performance play an extremely important role in cell adhesion, proliferation and differentiation. Therefore, it has become one of the important research directions in the field of tissue engineering,...
Claims
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