Super-porous polysaccharide microsphere and preparation method thereof

A technology of ultra-large pores and polysaccharides, applied in the field of biochemical industry, can solve problems such as low mass transfer efficiency, small pores, and slow separation speed

Active Publication Date: 2013-05-22
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to expand the application range of konjac glucomannan microspheres and dextran microspheres, and effectively solve the mass transfer problem caused by small pores, we propose a method for preparing super-large-porous polysaccharide microspheres by the micellar swelling method, and the prepared polysaccharide m

Method used

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  • Super-porous polysaccharide microsphere and preparation method thereof
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  • Super-porous polysaccharide microsphere and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Embodiment 1: Preparation of small molecule glucomannan

[0058] In a 1L beaker, mix 25ml of 0.1M HCl and 200g of deionized water evenly, pour 50g of konjac glucomannan powder into the mixture, stir evenly, and it will become jelly after swelling for 10min. Put it into a high-pressure reactor and degrade it at 120°C for 40min. After the degradation was complete, the reactor was taken out and allowed to cool to room temperature. Add 125g of 40% NaOH solution, stir to dissolve the jelly-like substance, and filter the impurities to obtain a clarified aqueous solution of konjac glucomannan of the desired concentration. The same method can prepare konjac glucomannan aqueous solution with solid content of 0.1%, 4%, 15%, 20%, 40% and so on for future use.

Embodiment 2

[0060] Accurately take by weighing 30g concentration be the konjac glucomannan (molecular weight is 50,000) aqueous solution of 0.05wt%, in aqueous solution, add the polyoxyethylene sorbitan monooleate that quality is 6g and 2g sodium lauryl sulfate in In a beaker, stir and mix for 1 h at 50°C in a constant temperature water bath. Accurately weigh 20g of polyglycerol fatty acid ester, 15g of dichloromethane and 45g of kerosene in a three-necked flask as the oil phase, stir in a constant temperature water bath at 50°C, and adjust an appropriate speed. Add the mixed water phase to the oil phase, disperse and emulsify for 40 minutes, then weigh 15g of epichlorohydrin crosslinking agent, and add it dropwise to the mixing system. Link 6h. After the reaction is finished, filter, wash the product several times with industrial ethanol and distilled water, and wash away components such as dichloromethane, kerosene, and surfactant to obtain the product. The appearance of the obtained ...

Embodiment 3

[0062]Accurately weigh 20 g of a 20% aqueous solution of konjac glucomannan (molecular weight: 150,000) and 2 g of polyoxyethylene sorbitan tristearate in a beaker, and stir and mix for 1 h in a constant temperature water bath at 30°C. Accurately weigh 15g of polyoxyethylene fatty acid ester, 50g of petroleum ether and 200g of turbine oil in a three-necked flask as the oil phase, stir in a constant temperature water bath at 30°C, and adjust the appropriate speed. Add the mixed water phase to the oil phase, disperse and emulsify for 1 hour, then weigh 30g of epichlorohydrin crosslinking agent, and add it dropwise to the mixing system. Joint curing 10h. After the reaction, filter, wash the product several times with industrial ethanol and distilled water, wash petroleum ether, turbine oil, surfactant and other components, and dry to obtain the product. The electron micrographs of the obtained microspheres are as follows: figure 2 shown. The average particle size of the micro...

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Abstract

The invention relates to a super-porous polysaccharide microsphere product and a preparation method thereof. The mean grain size of the microsphere is 1-500 microns and the mean bore diameter of the microsphere is controllable within a range of 90-800 nm. The preparation method of the super-porous polysaccharide microsphere comprises the following steps of: adding a high-content water-soluble surfactant to a water phase; dispersing the water-phase containing the surfactant to an oil phase to obtain large ports through oil-absorption swelling action of a micelle formed by the surfactant; and preparing the super-porous microsphere through crosslinking and curing of the larger pores. The product can be used as a separating and purifying medium, an immobilized enzyme carrier, a catalyst carrier or a high-efficiency adsorbent and is particularly suitable for being used as a separating medium for biological macromolecules.

Description

technical field [0001] The present invention relates to the field of biochemical industry, in particular to a super-large pore polysaccharide microsphere, in particular a super-large pore konjac glucomannan microsphere, a super-large pore dextran microsphere or a super-large pore konjac glucomannan-dextran microsphere and its preparation method. Background technique [0002] In recent years, with the rapid development of life science and technology, a large number of biological products have been introduced to the market, and biological products, including proteins, polypeptides, nucleic acids, etc., need to be analyzed, separated, and purified to meet quality requirements. However, due to the small pore size of conventional polysaccharide microspheres, taking the commonly used agarose medium as an example, its pore size is about 20-30nm, so that biological macromolecules can only pass through the medium by diffusion, resulting in slow mass transfer rate and separation. Lon...

Claims

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Application Information

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IPC IPC(8): C08L5/00C08L5/02C08B37/00C08B37/02C08J9/00C08J3/24
Inventor 马光辉周炜清苏志国陈娟李娟王伟宸
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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