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A kind of sericin conductive hydrogel and preparation method thereof and stent prepared therefrom

A technology of conductive hydrogel and sericin, which is applied in pharmaceutical formulations, prostheses, drug delivery, etc., to achieve the effects of reducing trauma and pain, excellent electrical conductivity, and good biocompatibility

Active Publication Date: 2018-11-09
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of the existing hydrogel materials in this field has good electrical conductivity, memory deformation ability and biological activity at the same time.

Method used

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  • A kind of sericin conductive hydrogel and preparation method thereof and stent prepared therefrom
  • A kind of sericin conductive hydrogel and preparation method thereof and stent prepared therefrom
  • A kind of sericin conductive hydrogel and preparation method thereof and stent prepared therefrom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-4

[0047] The preparation of embodiment 1-4 sericin conductive hydrogel

[0048] The preparation process of the sericin conductive hydrogel of the present embodiment is as follows: figure 1 shown.

[0049] 1. Preparation of sericin aqueous solution

[0050] The silk used in this example comes from the silkworm cocoon of the silkworm silk fibroin-deficient mutant (purchased from the Institute of Sericulture, Chinese Academy of Agricultural Sciences). An aqueous solution of sericin was prepared from the cocoons by the following method:

[0051] 1) Weigh 1 g of silkworm cocoon of mutant silkworm and cut it into 1 cm 2 The fragments were placed in a clean beaker, washed 3 times with ultrapure water, and centrifuged at 3500rpm for 5 minutes to remove water;

[0052] 2) Add 55 mL of LiBr aqueous solution with a concentration of 6 mol / L to the silkworm cocoon fragments obtained in step 1), put the beaker into a constant temperature water bath at 35° C. for 24 hours to dissolve sericin...

Embodiment 9-12

[0121] The preparation of embodiment 9-12 horseradish peroxidase (HRP) sustained-release agent

[0122] During the preparation of Example 1-4, the HRP solution was added so that the content of HRP in the final scaffold was 8 μg; the rest of the operations were the same as the preparation of the hydrogel in Example 1-4, thus obtaining four kinds of hydrogels containing HRP. These four sericin conductive hydrogels containing HRP were freeze-dried by the scaffold preparation method in Examples 5-8 to form the HRP slow-release agents of Examples 9-12.

[0123] Put the above slow-release agent in 1 mL of PBS with pH 7.4 at 37°C; , 264, 288, and 323 hours, take out the supernatant carefully, and add 1mL PBS (pH7.4) again; use enzyme-linked immunosorbent assay (ELISA) to measure the content of HRP in the supernatant, and calculate the accumulated HRP in the supernatant at each time point The ratio of the HRP content and the actual drug load is the cumulative release rate, the result...

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Abstract

The invention relates to a sericin conductive hydrogel, which is formed by cross-linking sericin protein, and the sericin conductive hydrogel contains 0.0125-0.0833g / mL sericin protein and 0.01-0.1mg / mL sericin protein carbon nanotubes. The invention also discloses a preparation method of the sericin conductive hydrogel, a bracket made of the sericin conductive hydrogel and application thereof. The sericin conductive hydrogel of the present invention and the scaffold prepared therefrom have excellent conductive properties, injectability and memory deformation properties, and the ability to promote nerve cell differentiation, so it is especially suitable for use in nerve, heart, muscle, etc. Wound repair and treatment of tissues closely related to physiological activities. The sericin conductive hydrogel of the present invention and the prepared scaffold and slow-release drug preparation can be transplanted into the body through injection or minimally invasive surgery, which minimizes the trauma and pain caused by the transplant operation for patients, The resulting risk of infection is also reduced, and there is no serious immune response after implantation in the body.

Description

technical field [0001] The invention relates to the field of biomaterials, more particularly, to a sericin conductive hydrogel, a preparation method thereof and a scaffold prepared therefrom. Background technique [0002] Hydrogel (Hydrogel) is a gel with water as the dispersion medium. There are hydrophobic groups and hydrophilic groups in the water-soluble polymer with network crosslinked structure, and the hydrophilic groups combine with water molecules to bind water molecules It is a cross-linked polymer that is connected inside the network and the hydrophobic groups swell with water. [0003] Sericin (Silk Sericin) is a natural macromolecular viscous protein wrapped on the surface of silk fibers, accounting for about 20-30% of the cocoon content, composed of polypeptides with a molecular weight of 24-400kDa, and its molecules are composed of serine, asparagus It is composed of 18 kinds of amino acids such as amino acid and glycine. In recent years, sericin has become ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/24C08J3/075C08L89/00C08K7/24A61L27/54A61L27/50A61L27/22A61L27/08A61L27/52
CPCA61L27/08A61L27/227A61L27/50A61L27/52A61L27/54A61L2400/16A61L2430/20A61L2430/30A61L2430/32C08J3/075C08J3/24C08J2389/00C08K7/24C08K2201/011C08L89/00
Inventor 王琳王征王健李晓麟杨文张磊
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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