Preparation of two antibody and antigen conjugates and method thereof for simultaneously detecting alpha-fetoprotein and carcinoembryonic antigen

Abstract

The invention relates to the field of medical tumor marker detection, in particular to preparation of two antibody and antigen conjugates respectively from 1,1'-ferrocenediacetic acid / gold@platinum and thionine / gold@ platinum and a method thereof for simultaneously performing specificity detection on alpha-fetoprotein and carcinoembryonic antigen. The 1,1'-ferrocenediacetic acid / gold@platinum and thionine / gold@ platinum are respectively used for preparing the two kinds of antibody and antigen conjugates; according to the method for simultaneously detecting the alpha-fetoprotein AFP and the carcinoembryonic antigen CEA by an electrochemical sensor to be built, a modified glassy carbon electrode is put into a mixed solution of the alpha-fetoprotein and the carcinoembryonic antigen; then, active sites which are not subjected to specific binding are sealed; after the glassy carbon electrode is taken out, alpha-fetoprotein antigen and carcinoembryonic antigen mixtures with different concentrations are sequentially used for modifying the glassy carbon electrode, so that the antigen and the antibody realize the specific bonding; a sandwich immunosensor is built; the sandwich immunosensor is used for detecting the concentration of the alpha-fetoprotein and carcinoembryonic antigen by using a DPV (differential pulse voltammetry) method.

Description

technical field [0001] The invention relates to the field of medical tumor marker detection, specifically a 1,1'-ferrocenedicarboxylic acid / gold@platinum (Fc / Au@Pt) nanocomposite and thionine / gold@platinum (Thi / Au@ Preparation of Pt) nanocomplex and its method for specific detection of alpha-fetoprotein and carcinoembryonic antigen simultaneously. Background technique [0002] Modern oncology believes that malignant tumors are a systemic disease that can appear in almost every organ and tissue of the human body except the heart and other individual organs, and some cancers have no obvious symptoms from the onset to the late stage. Diagnosis adds to the difficulty. In view of this, it is expected that the early diagnosis and treatment of cancer can be realized through the detection of tumor markers. Highly sensitive detection of tumor markers has high research value for the detection and treatment of cancer. (Freedland S., 2011. Cancer 117, 1123–1135.). However, the detect...

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Problems solved by technology

(Hu M., Yan J., He Y., Lu H., Weng L., Song S., Fan C., Wang L. ACS Nano 4 (2009) 488–494.) The detection methods of tumor markers are mainly There is ELISA (Wan, Y., Qi P., Zhang D., Wu J.J., Wang Y. 2012. Biosens. Bioelectron. 33, 69–74. and Jia, C.P., Zhong, X.Q., Hua, B. , Liu, M.Y., Jing, F.X., Lou, X.H., Yao, S.H., Xiang, J.Q., Jin, Q.H., Zhao, J.L. 2009. Biosens. Bioelectron. 24, 2836–2841.), chemiluminescent immunoassay (Zhang A.M., Xiang H.K., Zhang X., Guo W.W., Yuan E.H., Huang C.S., Jia N.Q., 2016. Biosens. Bioelectron. 75, 206–212. and Xu S.J., Liu Y., Wang T.H., and Li J.H. 2011. Anal. Chem., 83 (10), 3817–3823.) and immunofluorescence (Xie, Q.F., Weng X.H., Lu L.J., LinZ.Y., Xu X.W., Fu C.L. 2015. Biosens. Bioelectron. 77, 46–50. and Hua, W.H., Liua, Y.S., Yang, H.B., Zhou, X.Q., Li, C.M. 2011. Biosens. Bioelectron.26, 3683–3687.), etc., although these methods have the characteristics of sensitivity, accuracy, and high specificity, but they are not effective in environmental protection , operating speed, quantitative detection and other aspects have their own shortcomings; although some have been automated, due to the high price of special instruments and special reagents, they cannot be widely used in laboratories at present.

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