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Nano carrier capable of pH-responsive rapid release of object molecules, and preparation method and application thereof

A nano-carrier and guest molecule technology, applied in the field of nano-materials, to achieve the effect of easy operation, good stability and good stability

Active Publication Date: 2016-12-21
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, nanocarriers based on bio-peptides or bio-amino acids have not been able to achieve the explosive release of drugs without destroying the function of drug molecules.

Method used

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  • Nano carrier capable of pH-responsive rapid release of object molecules, and preparation method and application thereof
  • Nano carrier capable of pH-responsive rapid release of object molecules, and preparation method and application thereof
  • Nano carrier capable of pH-responsive rapid release of object molecules, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, the preparation of diphenylalanine nanocarrier

[0052] 1) Dissolve 1 mg of biological peptide diphenylalanine in 8 μl of hexafluoroisopropanol to obtain a hexafluoroisopropanol solution of biological peptide (also called solution a), with a concentration of 125 mg / ml, and store at 4°C for 24 hours above to make it fully dissolved;

[0053] 2) Dilute 2.4 μl of an aqueous solution of 25% glutaraldehyde by ultrapure water to 500 μl to obtain solution b with a concentration of 0.12%; the molar ratio of diphenylalanine to glutaraldehyde is 1 :1;

[0054] 3) Heat solution b in a water bath at 65°C until fully dissolved at a constant temperature, then add it to solution a, react with Schiff base, leave at room temperature (25°C) for 24 hours after aging, the solution turns into light yellow turbid liquid, and remove by centrifugation The supernatant liquid is the pH-responsive nanocarrier capable of rapidly releasing guest molecules of the present invention. ...

Embodiment 2

[0055] Embodiment 2, preparation of phenylalanine nanocarrier

[0056] 1) Dissolve 20 mg of biological amino acid phenylalanine in 1 mL of ultrapure water to obtain an aqueous solution of phenylalanine (also called solution a) with a concentration of 20 mg / ml, store at 4°C for more than 24 hours to fully dissolve;

[0057] 2) Take 24 μl of an aqueous solution of glutaraldehyde with a concentration of 25% by mass, i.e. solution b, with a concentration of 0.6%; the molar ratio of phenylalanine to glutaraldehyde is 1:1;

[0058] 3) Add solution b to solution a, react with Schiff's base, and after aging at room temperature (25°C for 24 hours, the solution turns into a light yellow turbid liquid, and centrifuge to remove the supernatant liquid to obtain the pH-responsive pH-responsive pH solution of the present invention. Nanocarriers for Rapid Release of Guest Molecules.

Embodiment 3

[0059] Embodiment 3, the characterization of the diphenylalanine nano-carrier obtained in Example 1 of the present invention

[0060] Scanning electron microscope characterization of the nano-carrier prepared in Example 1 of the present invention: drop the nano-container suspension (5 μl) that has been centrifuged and washed on the surface of the silicon wafer, and after vacuuming it to dryness, fix it on the sample stage with conductive glue, and After spraying Au particles on its surface with a radiometer, observe it under a HITACHI S-4800 scanning electron microscope, and the accelerating voltage is 10kV; the results are as follows figure 2 shown.

[0061] Depend on figure 2 It can be seen that the nano-carrier material obtained in the present invention is a monodisperse nano-sphere, the surface is very rough and has many pore structures.

[0062] Transmission electron microscopy characterization of diphenylalanine nanocarriers: drop the centrifuged-washed nanosphere su...

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Abstract

The invention discloses a nano carrier capable of pH-responsive rapid release of object molecules, and a preparation method and an application thereof. The nano carrier is prepared by carrying out a reaction of biological peptide or biological amino acid with a crosslinking agent; the biological peptide includes diphenylalanine and / or diphenylalanine carboxyl terminal amidated hydrochloride; the biological amino acid includes phenylalanine; the crosslinking agent comprises aliphatic dialdehyde. The preparation method comprises the following steps: mixing a biological peptide solution or a biological amino acid solution and a crosslinking agent solution, carrying out a Schiff base reaction, to obtain the nano carrier capable of pH-responsive rapid release of the object molecules. The nano carrier has the advantages of adjustable particle size, good stability and high encapsulation capability on medicines, and is capable of pH-responsive super rapid release.

Description

technical field [0001] The invention relates to a pH-responsive nano-carrier capable of rapidly releasing guest molecules, a preparation method and application thereof, and belongs to the field of nano-materials. Background technique [0002] The amino group of one amino acid is condensed with the carboxyl group of another amino acid to form a peptide molecule. Peptide molecules widely exist in organisms and participate in important life activities in cells or tissues. They have excellent biocompatibility and biodegradability, and are excellent raw materials for the preparation of various advanced biomaterials. Recently, it has been found that many peptide molecules (including cyclic peptides, dendritic peptides, amphiphilic peptides, copolypeptides and aromatic peptides, etc.) have very strong assembly properties and can easily form various functional ordered structures. It is of great research significance to design and synthesize new peptide molecules, use self-assembly ...

Claims

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Application Information

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IPC IPC(8): C07C251/08C07C249/02C07K5/065C07K1/02B82Y30/00B82Y40/00A61K47/18
CPCA61K47/183B82Y30/00B82Y40/00C07C249/02C07C251/08C07K5/06078
Inventor 李峻柏费进波张鹤秦晨晨
Owner INST OF CHEM CHINESE ACAD OF SCI
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