Dynamic multipoint grass variety identification and authentication method based on terahertz time domain spectroscopy

A terahertz time-domain, terahertz pulse technology, applied in the measurement of color/spectral properties, material analysis by optical means, measurement devices, etc., can solve the problems of long time, cumbersome process, expensive equipment, etc., to avoid fragmentation Effect

Active Publication Date: 2016-12-21
CHINA UNIV OF PETROLEUM (BEIJING) +1
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  • Abstract
  • Description
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Problems solved by technology

Among them, the DNA-based polymerase chain reaction detection technology mainly includes nucleic acid probe hybridization, DNA fingerprint analysis, PCR-RELP analysis, PCR specific amplification and other technologies, but the equipment of this method is too expensive, the process is cumbersome, and time-consuming Long, not suitable for large-scale detection in industry

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  • Dynamic multipoint grass variety identification and authentication method based on terahertz time domain spectroscopy
  • Dynamic multipoint grass variety identification and authentication method based on terahertz time domain spectroscopy
  • Dynamic multipoint grass variety identification and authentication method based on terahertz time domain spectroscopy

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Embodiment 1

[0078]The dynamic, multi-point identification and authentication method of grass varieties based on terahertz time-domain spectroscopy provided by the present invention can be detected by using a transmission terahertz time-domain spectroscopy device (THz-TDS), wherein a titanium-sapphire femtosecond mode-locked pulse laser generates A laser light source with an adjustable center wavelength of 810nm, a repetition rate of 82MHz, and a pulse width of 100fs; the laser pulses used in the above device are divided into two beams, one beam is used as pump light to excite GaAs photoconductive wires to generate THz pulses; the other One beam is used as the probe light, and the THz signal is measured by a crystal detector, and finally the THz signal is collected by a lock-in amplifier controlled by a chopper.

[0079] In order to prevent the influence of water vapor in the air on the THz signal, the optical path from generating the THz signal to the detection signal is sealed in a box fi...

Embodiment 2

[0126] In this embodiment, a multi-point and dynamic terahertz detection method is used to sample and detect the grassland No. 2 sample to be tested;

[0127] The grassland No. 2 sample to be tested in this example is sampled using the "sampling device for transmission-type terahertz detection (patent number is ZL 201520239272.7)"; wherein, the grassland No. 2 sample to be tested has 13 sampling points , the distribution of the 13 sampling points is as follows Figure 8 As shown; using a transmission-type terahertz time-domain spectrometer to sample and detect the grassland No. 2 sample to be tested, after the sampling and detection, perform data processing to obtain the average refractive index n(ω) and average absorption of the grassland No. 2 sample to be tested Coefficient α(ω) and other data, and then compare the average refractive index n(ω) and average absorption coefficient α(ω) data of Grassland No. 2 sample to be tested with the corresponding data of Grassland No. D...

Embodiment 3

[0135] In this embodiment, a single-point, static terahertz detection method is used to sample and detect the grassland No. 2 sample to be tested;

[0136] Grassland No. 2 samples to be tested in this embodiment are sampled using a conventional sampling device (i.e. a hollow box in the middle made of transparent polyethylene material in the prior art), that is, the Grassland No. 2 samples to be tested are placed in the On the sampling device, then place the sampling device at the position facing the terahertz pulse, and then emit a terahertz pulse for sampling detection. After sampling, take out the sampling device together with the sample, re-arrange the sample to another position, and then carry out The next sampling test; in the present embodiment, there are 13 sampling points for the grassland No. 2 sample to be tested, and a total of 13 samples are taken for the 13 sampling points. Sampling pulse, and then sampling detection;

[0137] After the sampling and testing, data...

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Abstract

The invention provides a dynamic multipoint grass variety identification and authentication method based on terahertz time domain spectroscopy. The method comprises the following steps: preparing a standard grass seed sample; respectively detecting the standard grass seed sample and dry nitrogen by using a terahertz time domain spectroscopy device to respectively obtain a terahertz pulse time domain waveform sum of the standard grass seed sample and the dry nitrogen, taking the terahertz pulse time domain waveform of the dry nitrogen as a reference signal, and taking the terahertz pulse time domain waveform of the standard grass seed sample as a sample signal; carrying out data processing on the terahertz pulse time domain waveform of the standard grass seed sample and the terahertz pulse time domain waveform of the dry nitrogen to construct a standard fingerprint spectrum library; and analyzing, calculating and establishing an identification database of unknown grass varieties according to optical parameters of the standard fingerprint spectrum library in combination with a mathematical statistical method, thereby carrying out qualitative analysis and recognition on unknown grass samples. By adoption of the method, the grass varieties can be effectively recognized and detected, the database can also be established for the unknown grass samples, and the unknown grass samples can be quickly distinguished and identified in batches.

Description

technical field [0001] The invention relates to a dynamic and multi-point identification and certification method for grass varieties based on terahertz time-domain spectroscopy, and belongs to the technical field of analysis and detection of grass seeds. Background technique [0002] Terahertz refers to electromagnetic waves with a frequency between 0.1THz-10THz and a wavelength between 30μm-1mm. Terahertz spectrum has the characteristics of typical pulse width in picosecond level and high time resolution; many organic molecules and biological macromolecules Both the vibrational and rotational energy levels fall in the THz band. These organic molecules and biomacromolecules have strong absorption and dispersion for terahertz, and the terahertz spectra of different substances often show different characteristics. Therefore, terahertz spectroscopy technology is used It can realize the feature recognition of many organic molecules and biological macromolecules, and has high te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35
CPCG01N21/3586
Inventor 王芳任慧赵景峰夏红岩郭帅王嘉妮
Owner CHINA UNIV OF PETROLEUM (BEIJING)
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