Extraction method of radix bardanae general flavone

An extraction method and technology of total flavonoids are applied in pharmaceutical formulations, medical preparations containing active ingredients, antidote and other directions, which can solve the problems of high cost of reagent consumables, low product yield, complicated process, etc. The effect of high yield and purity and broad application prospects

Inactive Publication Date: 2017-01-04
FUYANG HUAIBANG MACHINERY
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AI-Extracted Technical Summary

Problems solved by technology

However, at present, there are few techniques for extracting flavonoids from burdock, and most of them hav...
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Abstract

The invention belongs to the technical field of plant extraction, and discloses an extraction method of radix bardanae general flavone. The extraction method comprises the following steps of: step 1) drying and pulverizing; step 2) performing ultraviolet treatment; step 3) performing soaking treatment; step 4) performing ethanol extraction; step 5) performing flocculation; and step 6) performing extraction and drying. The extraction method is capable of extracting an effective substance general flavone compound from radix bardanae, greatly improving the yield and the purity, reducing the material cost and increasing the benefits of enterprises and has extensive application prospects.

Application Domain

Metabolism disorderAntinoxious agents +1

Technology Topic

ChemistryFlavones +3

Examples

  • Experimental program(3)

Example Embodiment

[0020] Example 1
[0021] A method for extracting total flavonoids from burdock root, comprising the following steps:
[0022] Step 1) Clean the fresh burdock root, then heat it in steam at 100°C for 20 minutes, take it out, cool it naturally, cut it into slices with a thickness of 3 mm, then dry it in an oven, pulverize it, and pass it through a 100-mesh sieve to obtain burdock root powder dry powder;
[0023] Step 2) Flatten the dry powder obtained in step 1) into a flat layer with a thickness of 5 mm, and then place it under ultraviolet light for 30 minutes, and the ultraviolet intensity is 3000 mW/cm 2;
[0024] Step 3) Soak the dry powder of step 2) in a 6wt% sucrose solution for 30min, collect the precipitate by centrifugation, and place the precipitate in a cool and ventilated place to dry;
[0025] Step 4) Extract the precipitate obtained in step 3) by refluxing with 75% ethanol for three times, each time for 30 minutes, and the ratio of material to liquid is 1g:10ml, combine the extracts, filter, and collect the filtrate;
[0026] Step 5) Add 1wt% flocculant to the filtrate in step 4), stir evenly, then stand for 40min, filter to remove the precipitate, and collect the supernatant;
[0027] Step 6) Concentrate the supernatant obtained in step 5) under reduced pressure into an extract with a density of 1.2 g/ml, then redissolve with 10 times the mass of purified water, extract twice with chloroform, combine the two aqueous phases, reduce Pressed and concentrated into an extract with a density of 1.3 g/ml, then diluted with twice the weight of purified water, passed through a polyamide column, eluted with 70% ethanol, collected the eluent, evaporated to recover ethanol, and freeze-dried to obtain a precipitate .
[0028] Determination of total flavonoids in the precipitate: Precisely draw 200 μL of the sample solution, place it in a 10 ml volume, measure the absorbance at 510 nm, and calculate the yield of total flavonoids as 24.3 mg/g burdock root dry powder, with a purity of 33.2%.
[0029] The flocculant is prepared according to the following process: (1) Peanut shell powder and sepiolite powder are pulverized to obtain peanut shell powder and sepiolite powder respectively, and peanut shell powder, sepiolite powder and diatomite are obtained by 2:1:1 The mass ratio of diatomite was mixed and stirred evenly, added to a sodium hydroxide solution with a concentration of 1M accounting for 5 times the weight of diatomite, stirred at 200 rpm for 120 min, then filtered, dried, and ground into a powder with a particle size of 10-20 mesh, That is component A; (2) Take chitosan and pectin according to the mass ratio of 10:1, mix and stir evenly, then add 2M sodium hydroxide aqueous solution that accounts for twice the mass of chitosan, ultrasonic at 30KHz for 10min, Finally, stir at 500 rpm for 3 minutes to obtain component B; (3) Mix and stir evenly component A and component B in a mass ratio of 2:1, then place it in a closed reaction kettle, and raise the temperature to a temperature of 2 °C/s. 150 ℃, heat preservation for 30 min, take out, put into a mixer, stir at 500 rpm for 3 min to obtain a mixture, put it in an oven, dry at 80 ℃ for 60 min, take out, and pulverize.

Example Embodiment

[0030] Example 2
[0031] A method for extracting total flavonoids from burdock root, comprising the following steps:
[0032] Step 1) Clean the fresh burdock root, then place it in 100 ℃ steam for heat treatment for 15 minutes, take it out, cool it naturally, cut it into slices with a thickness of 3 mm, then dry it in an oven, pulverize it, and pass it through a 100-mesh sieve to obtain burdock root powder dry powder;
[0033] Step 2) Flatten the dry powder obtained in step 1) into a flat layer with a thickness of 5mm, and then place it under ultraviolet light for 20 minutes, and the ultraviolet intensity is 3000mW/cm 2;
[0034] Step 3) Soak the dry powder of step 2) in a 6wt% sucrose solution for 30min, collect the precipitate by centrifugation, and place the precipitate in a cool and ventilated place to dry;
[0035] Step 4) Extract the precipitate obtained in step 3) by refluxing with 75% ethanol for three times, each time for 30 minutes, and the ratio of material to liquid is 1g:10ml, combine the extracts, filter, and collect the filtrate;
[0036] Step 5) Add 0.5wt% flocculant to the filtrate in step 4), stir evenly, then stand for 60min, filter to remove the precipitate, and collect the supernatant;
[0037] Step 6) Concentrate the supernatant obtained in step 5) under reduced pressure into an extract with a density of 1.2 g/ml, then redissolve with 10 times the mass of purified water, extract twice with chloroform, combine the two aqueous phases, reduce Pressed and concentrated into an extract with a density of 1.3g/ml, then diluted with twice the weight of purified water, passed through a polyamide column, eluted with 70% ethanol by volume, collected the eluate, evaporated to recover ethanol, and freeze-dried to obtain Precipitate.
[0038] Determination of total flavonoids in the precipitate: Precisely draw 200 μL of the sample solution, place it in a 10 ml volume, measure the absorbance at 510 nm, and calculate the yield of total flavonoids to be 23.9 mg/g burdock root dry powder, with a purity of 32.7%.
[0039]The flocculant is prepared according to the following process: (1) Peanut shell powder and sepiolite powder are pulverized to obtain peanut shell powder and sepiolite powder respectively, and peanut shell powder, sepiolite powder and diatomite are obtained by 2:1:1 The mass ratio of diatomite was mixed and stirred evenly, added to a sodium hydroxide solution with a concentration of 1M accounting for 5 times the weight of diatomite, stirred at 200 rpm for 120 min, then filtered, dried, and ground into a powder with a particle size of 10-20 mesh, That is component A; (2) Take chitosan and pectin according to the mass ratio of 10:1, mix and stir evenly, then add 2M sodium hydroxide aqueous solution that accounts for twice the mass of chitosan, ultrasonic at 30KHz for 10min, Finally, stir at 500 rpm for 3 minutes to obtain component B; (3) Mix and stir evenly component A and component B in a mass ratio of 2:1, then place it in a closed reaction kettle, and raise the temperature to a temperature of 2 °C/s. 150 ℃, heat preservation for 30 min, take out, put into a mixer, stir at 500 rpm for 3 min to obtain a mixture, put it in an oven, dry at 80 ℃ for 60 min, take out, and pulverize.

Example Embodiment

[0040] Example 3
[0041] Take the process of Example 1 as an example to detect the influence of each parameter and condition on the extraction efficiency:
[0042] Groups: control group 1, control group 2, example group 1;
[0043] Control group 1: do not use sucrose soaking and ultraviolet irradiation, other is the same as Example 1;
[0044] Control group 2: not treated with flocculant, treated with activated carbon, the others are the same as in Example 1.
[0045] The determination of total flavonoids in the precipitate is shown in Table 1:
[0046] Table 1
[0047] group Contentmg/g burdock root dry powder purity% Example 1 24.3 33.2 control group 1 14.6 29.7 control group 2 20.8 21.8

PUM

PropertyMeasurementUnit
Thickness3.0mm

Description & Claims & Application Information

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