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Preparation method and application of ultrasonic control type anti-tumor drug delivery system

An anti-tumor drug and delivery system technology, which is applied in the field of preparation of a sound-regulated anti-tumor drug delivery system, can solve the problems of drug leakage, low drug loading, low tumor response sensitivity, etc., and achieves significant economic and social benefits and is easy to use. The effect of industrial production and simple preparation process

Active Publication Date: 2017-01-04
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In view of the above problems, in order to solve the defects of the prior art, the purpose of the present invention is to provide a preparation method and application of an acoustically regulated anti-tumor drug delivery system, which can effectively solve the problem of low drug loading of existing tumor treatment drugs, and the delivery process will be difficult. There are problems of drug leakage, low tumor response sensitivity, slow release and easy drug resistance, and inability to regulate in vitro

Method used

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Examples

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Effect test

Embodiment 1

[0024] The ultrasound-regulated antitumor drug delivery system of the present invention specifically includes the following steps:

[0025] (1) Preparation of hollow mesoporous titanium dioxide

[0026] 0.1mol / LNH 3 ▪H 2 O 5-100ml dissolved in 400ml acetone, 8mL TiCl 4The acetone solution was added dropwise to the above solution, reacted at room temperature for 30min, centrifuged at 4000rpm for 12min, dried at 40°C to remove acetone, and then added excess methanol solution to make the NH in the reaction product 4 Cl crystallization and separation, removal of NH in the product 4 Cl crystals were vacuum-dried at 40°C for 24 hours to remove methanol to obtain hollow mesoporous titanium dioxide;

[0027] (2) Preparation of red blood cell membrane:

[0028] Take 5ml of female rat blood and add 100μL of heparin, centrifuge at 3500rpm at 4°C for 10min, collect the precipitated red blood cells, wash with 10ml of 1mol / LpH7.4 PBS buffer, place in 10ml of 0.25mol / LpH7.4 PBS buffer, ...

Embodiment 2

[0034] The ultrasound-regulated antitumor drug delivery system of the present invention specifically includes the following steps:

[0035] (1) Preparation of hollow mesoporous titanium dioxide:

[0036] 0.1mol / LNH 3 ▪H 2 O 5ml is dissolved in 350ml acetone to get NH 3 ▪H 2 O acetone solution to get NH 3 ▪H 2 O acetone solution, then 5mL TiCl 4 The acetone solution was added dropwise to the above solution, reacted at room temperature for 25min, centrifuged at 3000rpm for 8min, dried at 35°C to remove acetone, and then added excess methanol solution to make the NH in the reaction product 4 Cl crystallization and separation, removal of NH in the product 4 Cl crystals were vacuum-dried at 35°C for 24 hours to remove methanol to obtain hollow mesoporous titanium dioxide;

[0037] (2) Preparation of red blood cell membrane:

[0038] Take 2ml of female rat blood and add 40μL of heparin, centrifuge at 3500rpm for 8min at 4°C, collect the precipitated red blood cells, wash wi...

Embodiment 3

[0044] The ultrasound-regulated antitumor drug delivery system of the present invention specifically includes the following steps:

[0045] (1) Preparation of hollow mesoporous titanium dioxide:

[0046] 0.1mol / LNH 3 ▪H 2 O 100ml is dissolved in 450ml acetone to get NH 3 ▪H 2 O acetone solution to get NH 3 ▪H 2 O acetone solution, then 11mL TiCl 4 The acetone solution was added dropwise to the above solution, reacted at room temperature for 35min, centrifuged at 4000rpm for 12min, dried at 45°C to remove acetone, and then added excess methanol solution to make the NH in the reaction product 4 Cl crystallization and separation, removal of NH in the product 4 Cl crystals were vacuum-dried at 45°C for 24 hours to remove methanol to obtain hollow mesoporous titanium dioxide;

[0047] (2) Preparation of red blood cell membrane:

[0048] Take 5ml of female rat blood and add 100μL of heparin, centrifuge at 3500rpm at 4°C for 12min, collect the precipitated red blood cells, w...

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Abstract

The invention relates to a preparation method and application of an ultrasonic control type anti-tumor drug delivery system. The problems that existing tumor treatment drugs are low in drug loading capacity, are leaked when delivered, are low in tumor response sensitivity, slow in release and prone to causing drug resistance, and can not be controlled in vitro are solved effectively. The ultrasonic control type anti-tumor drug delivery system is established with hollow mesoporous titanium dioxide as the base body by being internally loaded with perfluorohexane and drug bleomycin, wrapped by an endogenous biological membrane-erythrocyte membrane and modified by tumor homing peptides; a nanoscale particle size distribution drug delivery system is prepared with hollow mesoporous titanium dioxide as the base body by being internally loaded with perfluorohexane and model drug bleomycin, wrapping surfaces of hollow mesoporous titanium dioxide by RBC, and being wrapped by an endogenous biological membrane-erythrocyte membrane; a tumor drug delivery system with a remote ultrasonic control function is established by modifying tumor homing peptide NGR tail ends with diacyl lipid.

Description

technical field [0001] The invention belongs to the field of medicine and chemical industry, in particular to a preparation method and application of a sound-regulated antitumor drug delivery system. Background technique [0002] At present, chemotherapy drugs are usually poorly soluble in water, have severe side effects, and are prone to drug resistance. The development of targeted and controlled release nano drug delivery system can solve the above problems to a certain extent. In recent years, scientists from various countries have carried out various researches in the field of nano-controlled release drug delivery systems, such as tumor-responsive pH-controlled release systems, near-infrared light-controlled release systems, etc. Although these nano-drug delivery systems can realize drug delivery in tumor sites Responsive release, but there are also some problems: such as low drug loading, drug leakage during delivery, low tumor response sensitivity, slow release and ea...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K38/14A61K9/00A61K47/02A61K47/46A61K47/48A61K47/06A61K49/22A61P35/00C01G23/047
CPCA61K9/0009A61K38/14A61K41/0033A61K47/02A61K47/06A61K47/46A61K49/221C01G23/047
Inventor 张振中张慧娟张晓戈祝杏张红岭侯琳
Owner ZHENGZHOU UNIV
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