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CeVO4 mimic enzyme material, and preparation method thereof and application

A technology of imitating enzymes and H2O2, applied in the field of imitating enzymes, can solve the problems of unexploited application potential in the biological field, and achieve the effects of good imitation peroxidase catalytic performance, easy control, stability and reusability

Inactive Publication Date: 2017-01-04
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, vanadic acid decoration (CeVO 4 ), as a composite metal oxide, has unique optical, electrical and redox properties, and has been widely used in the fields of luminescent materials, gas sensors, oxidation catalysts, photocatalysts and solid fuel cells. Untapped application potential

Method used

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  • CeVO4 mimic enzyme material, and preparation method thereof and application
  • CeVO4 mimic enzyme material, and preparation method thereof and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Rod CeVO 4 preparation of

[0023] 1.6mmolCe(NO 3 ) 3 ·6H 2 O and 2.0mmol ethylenediaminetetraacetic acid disodium (EDTA) were successively added to 30mL ultrapure water, and magnetically stirred to dissolve it to obtain solution A; meanwhile, 1.6mmol NH 4 VO 3 Add it into 30mL of ultrapure water, heat to 80°C and magnetically stir to dissolve it to obtain solution B; then add the above solution B dropwise to the above solution A under magnetic stirring to obtain a suspension, and then use 2.0mol / L NaOH solution to adjust the pH of the suspension to 10, then transfer the suspension to a stainless steel high-pressure reactor equipped with a polytetrafluoroethylene liner, and put it into an electric constant temperature blast drying oven for heat treatment at 180°C for 24h; After the end, the reactor was cooled to room temperature, and the precipitate obtained by suction filtration was washed with ultrapure water and absolute ethanol several times in sequen...

Embodiment 2

[0025] Example 2 Rod CeVO 4 preparation of

[0026] Prepared by hydrothermal synthesis, the difference from Example 1 is that the amount of EDTA is changed. 1.6mmolCe(NO 3 ) 3 ·6H 2 O and 1.5mmol disodium ethylenediaminetetraacetic acid (EDTA) were successively added to 30mL ultrapure water, and magnetically stirred to dissolve it to obtain solution A; simultaneously, 1.6mmol NH 4 VO 3 Add it into 30mL of ultrapure water, heat to 80°C and magnetically stir to dissolve it to obtain solution B; then add the above solution B dropwise to the above solution A under magnetic stirring to obtain a suspension, and then use 4.0mol / L NaOH solution to adjust the pH of the suspension to 10, then transfer the suspension to a stainless steel high-pressure reactor equipped with a polytetrafluoroethylene liner, and put it into an electric constant temperature blast drying oven for heat treatment at 180°C for 24h; After the end, the reactor was cooled to room temperature, and the precipit...

Embodiment 3

[0027] Prepared by hydrothermal synthesis method, the difference from Example 1 is that the pH value of the suspension is adjusted to 9. 1.6mmolCe(NO 3 ) 3 ·6H 2 O and 2.0mmol disodium ethylenediaminetetraacetic acid (EDTA) were successively added to 30mL ultrapure water, and magnetically stirred to dissolve it to obtain solution A; meanwhile, 2.0mmol NH 4 VO 3 Add it into 30mL of ultrapure water, heat to 80°C and magnetically stir to dissolve it to obtain solution B; then add the above solution B dropwise to the above solution A under magnetic stirring to obtain a suspension, and then use 3.0mol / L NaOH solution to adjust the pH of the suspension to 9, then transfer the suspension to a stainless steel high-pressure reactor equipped with polytetrafluoroethylene lining, and put it into an electric constant temperature blast drying oven for heat treatment at 180°C for 16h; After the end, the reactor was cooled to room temperature, and the precipitate obtained by suction filtr...

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Abstract

The invention discloses a CeVO4 mimic enzyme material. The material is CeVO4 with a rodlike structure and has a size of 50-800 nm. The invention further discloses a method for preparing the material and application thereof as a peroxidase. The material has good peroxidase catalytic simulation performance. The method for preparing the CeVO4 mimic enzyme material is simple in process, easy to control and low in cost.

Description

technical field [0001] The present invention relates to a CeVO 4 The simulated enzyme material and its preparation method and application belong to the field of simulated enzymes. Background technique [0002] Peroxidase (Horseradish peroxidase, referred to as HRP) is a type of oxidoreductase produced by microorganisms or plants. It uses hydrogen peroxide as an electron acceptor to catalyze the oxidation of substrates. It is often used to detect H 2 o 2 . h 2 o 2 is an important intermediate substance, the establishment involves H 2 o 2 The analysis method has always been a research direction that people are interested in. However, as a natural enzyme, peroxidase is also a protein with a supramolecular structure, which is composed of more than one hundred or even thousands of amino acids that fold or self-assemble through non-covalent interactions. Although it can efficiently and specifically catalyze various biochemical reactions and promote the metabolism of organi...

Claims

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Application Information

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IPC IPC(8): B01J23/22G01N21/33
CPCG01N21/33B01J23/22B01J35/50
Inventor 鞠鹏于延珍孙承君郑立曹为尹晓斐
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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