Method for extracting, purifying and detecting Euphausia superba DTDP

An Antarctic krill and extraction method technology, applied in the direction of measuring devices, organic chemistry, instruments, etc., can solve the problems of Antarctic krill DTDP, etc., and achieve the effect of simple extraction method, accurate separation and detection

Inactive Publication Date: 2017-01-04
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no research report on Antarctic krill DTDP in the world

Method used

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  • Method for extracting, purifying and detecting Euphausia superba DTDP
  • Method for extracting, purifying and detecting Euphausia superba DTDP
  • Method for extracting, purifying and detecting Euphausia superba DTDP

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Weigh 100 g of freeze-dried Antarctic krill, add 800 ml of methanol, and extract under reflux at 80 degrees Celsius for 7 times, each time for 1 hour, until the extract is colorless. After filtration, the filtrate was rotary evaporated to obtain a methanol paste. Add diethyl ether to the methanol paste for leaching 4 times, 1 hour each time, and filter to obtain the ether extract; the ether extract is concentrated by rotary evaporation to obtain the ether extract. Add 10 times the volume of ether to dissolve the ether extract, and then add 10 times the volume of 2% H 2 SO 4 The aqueous solution was stirred and extracted, and the layers were separated to obtain an acidic aqueous layer. Add an equal volume of chloroform to the acidic aqueous layer and stir for extraction 3 times, stirring for 1 h each time. After standing still, separate the layers to obtain the chloroform layer A. Add an equal volume of 2% NaOH aqueous solution to the chloroform layer A, stir and extr...

Embodiment 2

[0046] Weigh 100g of freeze-dried Antarctic krill, add 1000ml of methanol, and reflux at 80°C for 8 times, each time for 1.5h, until the extract is colorless. After filtration, the filtrate was rotary evaporated to obtain a methanol paste. Add diethyl ether to the methanol extract and extract 5 times, each time for 1h, and filter to obtain ether extract; the ether extract is concentrated by rotary evaporation to obtain ether extract. Add 10 times the volume of ether to dissolve the ether extract, and then add 20 times the volume of 1% H 2 SO 4 The aqueous solution was stirred and extracted, and the layers were separated to obtain an acidic aqueous layer. Add an equal volume of chloroform to the acidic aqueous layer and stir for extraction 4 times, stirring for 1 h each time. After standing still, separate the layers to obtain the chloroform layer A. Add an equal volume of 1% NaOH aqueous solution to the chloroform layer A, stir and extract 7 times, stir for 1 h each time, a...

Embodiment 3

[0049] Weigh 100 g of freeze-dried Antarctic krill, add 600 ml of methanol, and extract under reflux at 80 degrees Celsius for 9 times, each time for 2 hours, until the extract is colorless. After filtration, the filtrate was rotary evaporated to obtain a methanol paste. Add diethyl ether to the methanol paste for leaching 6 times, each time for 0.5h, and filter to obtain ether extract; diethyl ether extract is concentrated by rotary evaporation to obtain diethyl ether extract. Add 10 times the volume of ether to dissolve the ether extract, and then add 3% H 2 SO 4 The aqueous solution was stirred and extracted, and the layers were separated to obtain an acidic aqueous layer. Add an equal volume of chloroform to the acidic aqueous layer and stir for extraction 3 times, stirring for 1 h each time. After standing still, separate the layers to obtain the chloroform layer A. Add an equal volume of 3% NaOH aqueous solution to the chloroform layer A, stir and extract 7 times, sti...

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Abstract

The invention discloses a method for extracting, purifying and detecting Euphausia superba DTDP, comprising: contacting Euphausia superba with methanol, extracting, and filtering: evaporatively concentrating filtrate to obtain methanol paste; contacting the methanol paste with ether, extracting, and filtering; evaporating ether liquid to obtain ether extract; contacting the ether extract with ether, dissolving to obtain ether extract liquid, contacting the ether extract liquid with acid aqueous solution, and extracting to obtain an acid aqueous phase; contacting the acid aqueous phase with chloroform, extracting and separating to obtain chloroform layer A; contacting the chloroform layer A with sodium hydroxide aqueous solution, and extracting and separating to obtain alkali aqueous layer and chloroform layer B; evaporating the chloroform layer B to remove a solvent, adding petroleum ether for leaching, and filtering to obtain petroleum ether extract liquid; evaporating the petroleum ether extract liquid to remove a solvent to obtain DTDP. It is first time herein to discover DTDP in Euphausia superba, and DTDP is extracted and detected.

Description

technical field [0001] The invention relates to an Antarctic krill 5,10-dimethoxy-2,3,7,8-tetrahydro-1H,6H-pyrrolo[1,2-a; 1',2'-d]pyrrole The invention discloses an extraction, purification and detection method of oxazine, which belongs to the fields of food, medicine and chemical industry. Background technique [0002] Antarctic krill (Euphausia superba Dana), belonging to the phylum Arthropoda, Crustacea, and Order Krill, is small in size, generally about 5.5-6.0cm in length, and about 2g in weight. There are few species of Antarctic organisms, but the number is huge, and the food chain is relatively simple. Antarctic krill, which feeds on phytoplankton, is the main food of whales, seals, penguins and other carnivores, and is also the basis of the Antarctic food chain. Antarctic krill is one of the largest and most successful single species of biological resources on the earth, with a biological reserve of about 6.5×10 8 ~10×10 8 tons, the latest estimate is 3.79×10 8...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/04G01N30/02
CPCC07D487/04G01N30/02
Inventor 王翩翩刘代成
Owner SHANDONG NORMAL UNIV
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