Method for detecting l-type tryptophan concentration in solution
A tryptophan and L-type technology, applied in the direction of measuring devices, instruments, and electrochemical variables of materials, can solve the problems of high detection limit, low accuracy, low sensitivity, etc., and achieve low detection cost, simple and convenient operation, The effect of improving sensitivity and accuracy
Active Publication Date: 2019-06-07
SUZHOU ZHILVE INTELLECTUAL PROPERTY OPERATION CO LTD
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Problems solved by technology
[0004] Existing methods for detecting amino acids with different configurations include cyclic voltammetry and AC impedance. These methods only use one of the indicators as a basis for simultaneous qualitative and quantitative analysis. For example, cyclic voltammetry only uses the magnitude of the current signal as a method to distinguish different configurations. Amino acids, the AC impedance is distinguished by the resistance value, its accuracy is low, the sensitivity is low, and the detection limit is high
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Embodiment 1
[0046] Prepare the working electrode: immerse the reference electrode, auxiliary electrode and glassy carbon electrode in a mixed solution containing 2mg / mL aminated graphene quantum dots and 2mg / mLβ cyclodextrin, use cyclic voltammetry to scan and then take it out and dry it. The modified glassy carbon electrode;
[0047] Among them, the scanning parameters are set as: initial potential 0V, highest potential 1V, lowest point 0V, final potential 0V, scan rate 0.1V / s, scan times 100 times, sensitivity 10 -4 A / V, waiting time is 2s.
[0048] Working electrode pretreatment: place the modified glassy carbon electrode on a polishing cloth containing polishing powder with a particle size of 0.3 μm and polish it to the mirror surface, and then place the glassy carbon electrode in methanol and H at a concentration of 0.5 mol / L in sequence. 2 SO 4 The solution and ultrapure water were sonicated for 30 s respectively, and rinsed with ultrapure water for 1 min after each sonication to ...
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Abstract
The invention discloses a method for detecting the concentration of L-type tryptophan in a solution. The method comprises the following steps: detecting L-type tryptophan in a solution to be detected by using a three-electrode system by virtue of differential pulse voltammetry, and obtaining the concentration of the L-type tryptophan in the solution to be detected according to a linear equation of the L-type tryptophan, wherein a working electrode in the three-electrode system is a modified glassy carbon electrode obtained by modifying a glassy carbon electrode with aminated graphene quantum dots and beta cyclodextrin. By adopting the method, the concentration of the L-type tryptophan can be rapidly detected, and the method is high in sensitivity and high in accuracy. According to the method, a related sensation interface is established as a three-electrode system sensor for detection, tryptophan configuration positioning is implemented by using potential, and quantification is implemented by using a peak current value, so that the L-type tryptophan detection sensitivity and the accuracy are improved, and 8.5*10<-7> mol / L L-type tryptophan can be detected at least. By adopting the method disclosed by the invention, the detection cost is greatly lowered, and the operation is simple and convenient.
Description
technical field [0001] The invention relates to the technical field of isomeric amino acid detection. More specifically, the present invention relates to a method for detecting the concentration of L-tryptophan in a solution by differential pulse voltammetry based on the electrochemical sensing of aminated graphene quantum dots and β-cyclodextrin modification. technical background [0002] Tryptophan is an important precursor substance for auxin biosynthesis in plants. Its structure is similar to indole acetic acid, and it is ubiquitous in higher plants. Auxin can be synthesized from tryptophan. This product is an important nutritional agent. It can participate in the renewal of plasma proteins in animals, and can promote riboflavin to play a role. It can also help the synthesis of niacin and heme. It can significantly increase the antibodies in the fetuses of pregnant animals, and it can promote lactation for lactating cows and sows. effect. When livestock and poultry l...
Claims
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IPC IPC(8): G01N27/48G01N27/30
CPCG01N27/308G01N27/48
Inventor 黄珊肖琦卢双燕
Owner SUZHOU ZHILVE INTELLECTUAL PROPERTY OPERATION CO LTD