A Plant Weak Electric Signal Amplifier

Abstract

The invention relates to a weak plant electrical signal amplifier comprising an isolation variable resistance module, a differential amplification module, a second level amplification module, a filter module and a zero setting and power amplification module, wherein a collected weak plant electrical signal is transmitted to the isolation variable resistance module, an output end of the isolation variable resistance module carries out amplifying and filtering processing on the weak plant electrical signal through the differential amplification module, the second level amplification module and the filter module in sequence, and the weak plant electrical signal is transmitted to the power amplification module to complete the amplification of the power amplification module. The weak plant electrical signal amplifier provided by the invention can effectively amplify microvolt level plant voltage signals and can collect the plant voltage signals in multiple types, and the sensitivity is high.

Description

technical field [0001] The invention relates to a signal amplifier, in particular to a plant weak electric signal amplifier. Background technique [0002] So far, there have been many studies on action potentials in plants. Studies have found that action potentials generally exist in unicellular and multicellular algae, lower plants, and higher plants; studies on the formation mechanism of plant action potential ions suggest that calcium ions, chloride Ions, potassium ions, and proton pumps are all involved; light stimulation, voltage and current stimulation, mechanical stimulation, glutamate, heat stimulation, low temperature stimulation, etc. can all trigger the generation of action potentials in plants; the stimulation of action potentials needs to reach a certain level. The threshold of action potential can trigger the generation of action potential, and its magnitude does not increase with the increase of stimulation intensity. In addition, the generation of plant actio...

AI Technical Summary

Problems solved by technology

In the early days, due to limited conditions, people used polygraphs, oscilloscopes, and general-purpose amplifiers to study plant electrical signals. However, due to limited precision, they could only investigate plant electrical signals at the millivolt level. The impedance of oscilloscopes and general-purpose amplifiers generally cannot meet the needs of plants. The demand for high impedance, therefore, due to the unsatisfactory accuracy and input impedance, the collection and analysis of plant electrical signals are greatly limited. In addition, due to the limited number of channels of these devices, it is impossible to collect a large amount of data at the same time

[0005] With the development of technology, due to the needs of animal physiological experiments, biological function experiment systems with high precision and relatively complete functions have appeared, but the amplifier impedance of general biological function experiment systems can only meet the impedance requirements of animal cell electrical signal measurement, while plant Cell impedance is higher than that of animal cells, so it cannot meet the needs of plant electrical signal acquisition in terms of effective signal acquisition; due to the low-frequency characteristics of plant electrical signals, which are different from the main frequency distribution of animal electrical signals, too many acquisition frequencies The domain will introduce unnecessary noise, so it cannot meet the needs of plant electrical signal acquisition in terms of frequency. In addition, the biological function experiment system is expensive

[0006] Non-destructive detection method (MIFE system Microelectrode ion flux measurement and SIET system Scanning ion-selective electrode technique): According to the characteristics of the diffusion of substances in the solution, the ion activity of the cell can be measured without destroying the cell, although it is non-destructive However, it has extremely high requirements on the accuracy of the distance between electrodes and the performance of the electrodes, and must rely on micromanipulation. Therefore, it is difficult to measure under the natural growth conditions of plants, and due to space constraints, it is impossible to simultaneously measure multiple cells. Measurements, i.e. inability to perform multi-dimensional stereo measurements of plant electrical signals

In addition, the price of this system can be expensive, mainly relying on imports

[0007] Patch clamp technology: Since Neher (Nel) and Sakmann (Sakmann) invented the patch clamp technology based on the voltage clamp technology in 1980, the patch clamp technology has been widely used in the field of plant electrophysiology, helping people discover a series of plant ions channel, its measurement modes can generally be divided into four types, namely: attached recording, whole-cell recording, intima-out recording and adventitia-out recording; since the patch clamp technique is suitable for single-cell measurement, and the electrodes need to be in The signal is measured on the cell membrane, but plant cells have cell walls, so this technique is more suitable for measuring animal cells or plant cells (protoplasts) with cell walls removed, and cannot be applied to certain tissues of plants or whole plants electrical signal measurement

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