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Preparation method of antibacterial acellular dermal matrix dressing material

A technology of acellular dermis and dermal matrix, which is applied in the field of preparation of antibacterial acellular dermal matrix dressings, can solve the problems of low antibacterial performance, limit the wide application of acellular dermal matrix dressings, and poor biocompatibility, and achieve low antigenicity Effect

Inactive Publication Date: 2017-01-11
GUANGDONG TAIBAO MEDICAL SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, acellular dermal matrix (ADM) dressings prepared by traditional methods have disadvantages such as poor permeability, low antibacterial performance, and poor biocompatibility, which severely limit the wide application of acellular dermal matrix dressings in clinical practice.

Method used

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  • Preparation method of antibacterial acellular dermal matrix dressing material
  • Preparation method of antibacterial acellular dermal matrix dressing material
  • Preparation method of antibacterial acellular dermal matrix dressing material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Peel off the skin of healthy newborn pigs and remove the hair; use a skin remover to make a sliced ​​skin with a thickness of 0.8mm; use laser drilling technology to make holes in the sliced ​​skin with a hole diameter of 0.3mm and a hole distance of 2mm ; Then use cobalt 60 to irradiate and sterilize the tomographic skin slices, the dose is 30kGy, and the irradiation time is 12h; the sterilized dermis matrix is ​​placed in a -40°C ultra-low temperature refrigerator for 3h, and then placed in - Pre-freeze in a 75°C ultra-low temperature refrigerator for 3 hours, take it out, put it in a 37°C water bath to thaw for 1 hour, and freeze and thaw repeatedly 3 times; add the freeze-thawed dermal matrix to the buffer and clean it with ultrasonic waves, and the frequency of ultrasonic shock treatment 5 times, each time for 30 minutes; then use a mass concentration of 1.0% NaOH solution and a mass concentration of 0.4% ammonium lauryl sulfate anionic surfactant mixture to degreas...

Embodiment 2

[0026] Peel off the skin of healthy newborn pigs and remove the hair; use a skin remover to make a sliced ​​skin with a thickness of 0.5 mm; use laser drilling technology to make holes in the sliced ​​skin with a hole diameter of 0.1 mm and a hole distance of 1.5 mm; then sterilize the sliced ​​skin slices by irradiation with cobalt 60, the dose is 25kGy, and the irradiation time is 10h; the sterilized dermal matrix is ​​pre-frozen in a -30℃ ultra-low temperature refrigerator for 2h, and then placed in Take it out after being pre-frozen in the ultra-low temperature refrigerator at -70°C for 2 hours, thaw it in a 37°C water bath for 1 hour, and freeze and thaw twice; add the freeze-thawed dermal matrix into the buffer and clean it with ultrasonic waves The frequency is 3 times, each time for 30 minutes; then use a mass concentration of 1.0% NaOH solution and a mass concentration of 0.1% fatty alcohol sodium isethionate anionic surfactant mixture to degrease the cleaned dermal ma...

Embodiment 3

[0028] Peel off the skin of healthy newborn pigs and remove the hair; use a skin remover to make a sliced ​​skin with a thickness of 1.5 mm; use laser drilling technology to make holes in the sliced ​​skin with a hole diameter of 0.5 mm and a hole distance of 2.5 mm; then sterilize the tomographic skin slices with cobalt 60 irradiation, the dose is 50kGy, and the irradiation time is 15h; the sterilized dermal matrix is ​​pre-frozen in a -50℃ ultra-low temperature refrigerator for 3h, and then placed in Take it out after being pre-frozen in the ultra-low temperature refrigerator at -80°C for 3 hours, thaw it in a water bath at 37°C for 1 hour, and freeze and thaw repeatedly 4 times; The frequency is 7 times, each time for 30 minutes; then degrease the cleaned dermal matrix with a mass concentration of 2.0% NaOH solution and a mass concentration of 0.6% dodecyl phosphate anionic surfactant mixture; Decellularize the decellularized dermal matrix with 3.0% TritonX-100; then soak t...

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Abstract

The invention belongs to the technical field of biological materials of tissue engineering, and particularly relates to a preparation method of an antibacterial acellular dermal matrix dressing material. The preparation method comprises the following steps: selecting a healthy mammal, stripping the skin of the mammal, removing hair, preparing split-thickness skin, performing laser drilling, performing low temperature freeze thawing, performing ultrasonic oscillation, performing degreasing treatment, performing acellular treatment, soaking water-soluble chitosan, performing freeze drying, packaging and performing irradiation sterilization, thus obtaining the antibacterial acellular dermal matrix dressing material. The antibacterial acellular dermal matrix dressing material disclosed by the invention has a collagen three-dimensional entire structure, is thorough in decellularization and short in manufacture cycle, and has the characteristic of antibacterial function.

Description

technical field [0001] The invention relates to the technical field of biological materials, in particular to a preparation method of an antibacterial acellular dermal matrix dressing. Background technique [0002] The epidermis, dermis, and subcutaneous tissue together constitute the skin structure of the human body. As one of the most important organs in the human body, the skin covers the surface of the human body and has defense functions, sensory functions, immune functions, absorption functions, body temperature regulation functions, excretion functions and secretion and other functions. When human skin is damaged, especially skin burns, it is easy to cause various damages to the body after burns, such as immune system imbalance, excessive loss of water, protein and other cell components, and increased metabolism. Severe burns can even be life-threatening. Therefore, wound dressing must be used to cover the wound surface after injury, which can temporarily protect the...

Claims

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Application Information

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IPC IPC(8): A61L15/40A61L15/28A61L15/42A61L15/46
CPCA61L15/40A61L15/28A61L15/42A61L15/425A61L15/46C08L5/08
Inventor 卢亢陈锦涛吴帅
Owner GUANGDONG TAIBAO MEDICAL SCI TECH
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