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Preparing method of pearl layer powder - chitosan porous scaffold

A technology of nacre powder and porous scaffold, which is applied in medical science, tissue regeneration, prosthesis and other directions, can solve problems such as poor mechanical properties, and achieve the effects of low production cost, good mechanical properties and good biological safety.

Inactive Publication Date: 2017-01-11
SHAANXI YIPINDA PETROCHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CS has good biocompatibility and can promote wound healing, but its mechanical properties are poor due to its own porous structure.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation method of nacre powder-chitosan porous support comprises the following steps:

[0019] (1) Take 2.0g of chitosan, add it to 100 mL of acetic acid solution with a concentration of 0.1 mol / L, stir for 12 hours to obtain a chitosan solution, and let it stand for 24 hours;

[0020] (2) Weigh 14.0g sodium β-glycerophosphate, add distilled water to make up to 25mL, mix well to obtain sodium β-glycerophosphate solution;

[0021] (3) Mix the solutions of step (1) and step (2) according to the volume ratio of 7:1, stir for 2 hours under ice bath, and adjust the pH=6.5 with 36% acetic acid and 4% sodium hydroxide solution;

[0022] (4) Add nacre powder, which has the same quality as chitosan. After stirring, ultrasonicate for 2 hours at a power of 200W, stir for 48 hours in an ice bath, pour into the mold and let stand at -20°C for 12 hours, then place Freeze-dry at -20°C for 48 hours in a vacuum freeze dryer, and sterilize to obtain nacre powder-chitosan porous ...

Embodiment 2

[0024] The preparation method of nacre powder-chitosan porous support comprises the following steps:

[0025] (1) Take 2.0g of chitosan, add it to 100 mL of acetic acid solution with a concentration of 0.1 mol / L, and stir for 12 hours, wherein the stirring speed is 500rpm to obtain a chitosan solution, and let it stand for 36 hours;

[0026] (2) Weigh 14.0g sodium β-glycerophosphate, add distilled water to make up to 25mL, mix well to obtain sodium β-glycerophosphate solution;

[0027] (3) Mix the solutions of step (1) and step (2) according to the volume ratio of 7:1, stir for 2 hours under ice bath, and adjust the pH=7.5 with 36% acetic acid and 4% sodium hydroxide solution;

[0028] (4) Add nacre powder, which has the same quality as chitosan. After stirring, ultrasonicate for 2 hours at a power of 200W, stir for 48 hours in an ice bath, pour into the mold and let stand at -20°C for 12 hours, then place Freeze-dry at -20°C for 48 hours in a vacuum freeze dryer, and sterili...

Embodiment 3

[0030] The preparation method of nacre powder-chitosan porous support comprises the following steps:

[0031] (1) Take 2.0 g of chitosan, add it to 100 mL of acetic acid solution with a concentration of 0.1 mol / L, and stir for 12 hours, wherein the stirring speed is 500 rpm to obtain a chitosan solution, and let it stand for 30 hours;

[0032] (2) Weigh 14.0g sodium β-glycerophosphate, add distilled water to make up to 25mL, mix well to obtain sodium β-glycerophosphate solution;

[0033] (3) Mix the solutions of step (1) and step (2) according to the volume ratio of 7:1, stir for 2 hours under ice bath, and adjust the pH=7.0 with 36% acetic acid and 4% sodium hydroxide solution;

[0034] (4) Add nacre powder, which has the same quality as chitosan. After stirring, ultrasonicate for 2 hours at a power of 200W, stir for 48 hours in an ice bath, pour into the mold and let stand at -20°C for 12 hours, then place Freeze-dry at -20°C for 48 hours in a vacuum freeze dryer, and steri...

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PUM

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Abstract

The invention discloses a preparing method of pearl layer powder - chitosan porous scaffold, comprising the steps of (1) adding chitosan into acetic acid solution to obtain chitosan solution and standing for 24-36 h; (2) dissolving sodium Beta-glycerophosphate in distilled water to obtain the sodium Beta- glycerophosphate solution; (3) mixing the solutions from step (1) and step (2) at the volume ratio of 7:1 and stirring for 2 h under ice bath, and adjusting pH with acetic acid and sodium hydroxide solution; (4) adding nacre powder and stirring for 48 h under ice bath, pouring into mould, standing, lyophilizing and sterilizing to obtain nacre powder / chitosan porous scaffold. The compound scaffold has porous structure with uniform pore size, good biological safety and good mechanical properties, and can be applied as tissue engineering scaffold of non-bearing part in the course of repairing tissue defect.

Description

technical field [0001] The invention belongs to the field of medical material preparation, in particular to a preparation method of pearl layer powder-chitosan porous support. Background technique [0002] Preparation of suitable bioactive in vivo substitute materials to help repair human defective tissues has always been a hot spot in tissue engineering research. Nacre powder (NP) is the powder ground from the innermost layer of gastropod mollusk shells. Its main components are 95% aragonite calcium carbonate and 5% organic matter such as protein, glycoprotein, and polysaccharide. NP is widely favored by researchers in the fields of food technology and medicine. The research on compounding multiple drugs with NP has achieved relatively satisfactory results. In recent years, the osteogenic properties of NP have gradually attracted the attention of tissue engineering researchers. NP has good biocompatibility, osteoconduction and potential osteoinductivity. It is a new type o...

Claims

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Application Information

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IPC IPC(8): A61L27/44A61L27/56
CPCA61L27/446A61L27/56A61L2430/02
Inventor 李长英
Owner SHAANXI YIPINDA PETROCHEM CO LTD
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