Nostoc commune polysaccharide extraction method
A fungus and polysaccharide technology is applied in the field of extraction of fungus polysaccharide, which can solve the problems of low polysaccharide extraction rate, complex preparation process, and high loss of raw materials, and achieves simple operation method, high extraction rate and recovery rate, and reduced raw material cost. Effect
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Embodiment 1
[0052] A method for extracting polysaccharides from fungus, such as figure 1 shown, including the following steps:
[0053] (1) Preparation of ground fungus powder suspension: 100 g of ground fungus powder was dissolved in [Bmim]Cl ionic liquid with a concentration of 0.4 mol / L, and extracted for 30 minutes to prepare ground fungus powder suspension.
[0054] (2) Preparation of ground fungus cell crushing solution: use ultrasonic-microwave combined reaction system to crush the ground fungus powder cells in the prepared ground fungus powder suspension, set the ultrasonic power to 150w, ultrasonic treatment time 8min; microwave power to 160w , microwave heating treatment time 3min, during microwave heating treatment, adopt intermittent treatment mode, heating 1min, intermittent 1min, centrifugation treatment 15min with the speed of 3500rpm afterwards, take supernatant, obtain the ground fungus cell broken liquid.
[0055] (3) Preparation of crude extract of Auricularia auricula...
Embodiment 2
[0062] Weigh 100g of ground fungus powder, add 15 times the amount of 0.3mol / L ionic liquid, and extract at room temperature for 30 minutes; at 100w ultrasonic power, 5min ultrasonic time, 150w microwave power, microwave heating adopts intermittent treatment, heating 1min, intermittent 1min, microwave Treat for 3 minutes, centrifuge at 3000 rpm for 15 minutes to obtain supernatant;
[0063] Add ammonium sulfate to the supernatant to make the saturation 70%, let stand at 4°C for 2 hours, centrifuge at 5000 rpm for 10 minutes, and take the supernatant to obtain the crude extract of Auricularia polysaccharide;
[0064] The crude polysaccharide extract and the reverse micelles solution were fully mixed according to V / V=1:1, extracted for 30 minutes, and centrifuged at 2000rpm for 5 minutes to obtain the reverse micelles phase; the reverse micelles solution was chloroform with a volume ratio of 1:1 After mixing with n-butanol, add TOMAC to make a mixed solution with a final concent...
Embodiment 3
[0069] Weigh 100g of ground fungus powder, add 25 times the amount of 0.7mol / L ionic liquid, and extract at room temperature for 50 minutes; at 200w ultrasonic power, 10min ultrasonic time, 200w microwave power, microwave heating adopts intermittent treatment, heating 1min, intermittent 1min, microwave Treat for 5 minutes, centrifuge at 5000 rpm for 20 minutes to obtain supernatant;
[0070] Add ammonium sulfate to the supernatant to make the saturation 70%, let stand at 8°C for 2 hours, centrifuge at 7000rpm for 15 minutes, and take the supernatant to obtain the crude extract of Auricularia polysaccharide;
[0071] The crude polysaccharide extract and the reverse micelles solution were fully mixed according to V / V=2:1, extracted for 60 minutes, and centrifuged at 3000rpm for 10 minutes to obtain the reverse micelles phase; wherein the reverse micelles solution was chloroform with a volume ratio of 4:1 After mixing with n-butanol, add TOMAC to make a mixed solution with a conc...
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