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Structural analysis method of beta-glucan

A technology for structural analysis and dextran, applied in the field of chemical analysis, can solve the problems of difficulty in obtaining high-resolution spectra, affecting the measurement results, harsh measurement conditions, etc., achieving strong operability, reducing the desorption process, and high accuracy Effect

Inactive Publication Date: 2017-01-11
NINGBO XINUOYA MARINE BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the related equipment of infrared spectroscopy and nuclear magnetic resonance is expensive, the cost is very high, the measurement conditions are harsh, the equipment requirements are high, and it is not suitable for general laboratory operations; and β-glucan is a macromolecular polysaccharide structure with high viscosity and low solubility. High, which directly affects the measurement results of infrared spectroscopy and nuclear magnetic resonance, it is difficult to obtain high-resolution spectra, which causes certain difficulties in solving the spectrum

Method used

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  • Structural analysis method of beta-glucan

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Embodiment 1

[0028] One, the reagent involved in this embodiment and its preparation are as follows:

[0029] ①Preparation of 0.1mol / L PH 7~8 phosphate buffer solution: Weigh 4.644g of anhydrous sodium dihydrogen phosphate (NaH 2 PO 4 ) and 21.945g disodium hydrogen phosphate dodecahydrate (Na 2 HPO 4 12H 2 0), put it in a beaker, and add a small amount of distilled water to dissolve it. After all the solids are dissolved, transfer the solution to a 1000ml volumetric flask. Rinse the beaker for dissolving with distilled water three times, and add the water after rinsing into the volumetric flask. Tolerance, that is.

[0030] ② Alkaline copper tartrate solution: weigh 15g of copper sulfate (CuSO 4 ·5H 2 O) and 0.05g methylene blue, dissolved in distilled water and diluted to 1000ml.

[0031] ③Alkaline copper tartrate B solution: Weigh 50g potassium sodium tartrate and 75g sodium hydroxide (NaOH), dissolve in distilled water, then add 4g potassium ferrocyanide [K 4 Fe(CN) 6 ], after...

Embodiment 2

[0058] The reagent preparation and preliminary treatment of this embodiment are the same as those in Embodiment 1.

[0059] This embodiment is a method for analyzing the structure of β-glucan, comprising the following steps:

[0060] (4) take m 0 The sample to be tested in parts by weight is dissolved in water to form V m ml solution, 2mg≤m 0 ≤10mg; draw 5.00ml each of alkaline copper tartrate solution A and solution B, put them in a conical flask, add 10ml of distilled water, add 3 glass beads, add the sample solution dropwise from the burette at a fast and then slow speed, and titrate Keep the solution boiling. When the solution turns from blue to light, titrate at a rate of 1 drop every 2s until the blue color of the solution just fades as the end point, and record the volume V consumed by the sample solution 0 ; Parallel operation 3 times, get its average value, draw the reducing sugar amount (in glucose, %) of untreated sample

[0061] x 0 ...

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Abstract

The invention provides a structural analysis method of beta-glucan. The structural analysis method comprises the following steps: (1) measuring the reducing sugar quantity x0 by using an alkaline cupric tartrate solution titration method; (2) measuring the reducing sugar quantity x1 of a sample treated by using amylase by the alkaline cupric tartrate solution titration method; (3) measuring the reducing sugar quantity x2 of a sample treated by helicase by using the alkaline cupric tartrate solution titration method; (4) dissolving a to-be-measured sample in water, and adding enough sodium periodate for reacting; after complete reaction, adding ethylene glycol to break excess sodium periodate; measuring the optical density of the solution at a wavelength of 223nm; and calculating the consumption amount n1 mole of periodic acid; and (5) taking the solution obtained by the step (4), performing titration with a sodium hydroxide solution, and calculating the formic acid release amount n2 mole. Through calculation, the beta-glucan linked by 1,3- and 1,6-glucosidic bonds and 1,3- and 1,4-glucosidic bonds can be identified by a chemical analysis method.

Description

【Technical field】 [0001] The present invention relates to a chemical analysis method, in particular to a structural analysis method of β-glucan. 【Background technique】 [0002] There are a wide range of sources of β-glucan. The main sources of β-glucan on the market are oat glucan, fungal glucan, seaweed glucan, yeast glucan, etc. β-glucans from different sources have different structures, resulting in different biological activities. There are two structures of β-glucan: one is a mixture of β-1,3-glucan and β-1,4-glucan, such as oat β-glucan; the other is based on 1, β-glucans with 3-glycosidic bonds as the main chain and 1,6-glycosidic bonds as branched chains, such as fungal glucan, seaweed glucan, etc. The research results show that β-glucan linked by 1,3-, 1,6-glycosidic bonds has multiple functions such as inhibiting tumors, activating the body's immunity, anti-aging, anti-allergic, and stabilizing intestinal flora. More than oat glucan linked by 1,3-, 1,4-glycosidi...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N21/78
CPCG01N21/33G01N21/78
Inventor 贾艳
Owner NINGBO XINUOYA MARINE BIOTECH CO LTD
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