Retina containing new zealand spinach polypeptide
A technology of apricot and eye mask, which is applied in the field of fish collagen moisturizing mask and its preparation, can solve the problems of unreported and low molecular weight, and achieve the effect of alleviating dark circles, alleviating edema, and expanding the optional types
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Embodiment 1
[0041] Take 500g of apricots, wash them, make a slurry, add 0.01mol / L sodium chloride solution 15 times the amount of apricots, ultrasonically extract at 40°C for 30 minutes, centrifuge at 3500r / min to get the supernatant, add citric acid to adjust the pH To 4.3, centrifuge at 3500r / min to collect the precipitate, wash with water, incubate at 80°C for 8min, centrifuge at 3200r / min to collect the precipitate, add an appropriate amount of neutral phosphate buffer solution to completely dissolve the precipitate, add 3% neutral protease, and incubate at 48°C Decompose for 8 hours, inactivate the enzyme at 70°C for 5 minutes, add citric acid to adjust the pH to 5.1, add 6% compound enzyme, enzymolyze at 50°C for 7 hours, inactivate the enzyme for 10 minutes at 85°C, filter with ultrafiltration membrane, and separate the molecular weight of 500Da-5000Da Apricot peptides for use.
Embodiment 2
[0043] Take 500g of apricot, wash it, beat it into a slurry, add 0.01mol / L sodium chloride solution of 15 times the amount of apricot, ultrasonically extract at 50°C for 20min, centrifuge at 4000r / min to get the supernatant, add citric acid to adjust the pH Centrifuge at 5,3300r / min to collect the precipitate, wash with water, incubate at 83°C for 5min, centrifuge at 3000r / min to collect the precipitate, add an appropriate amount of neutral phosphate buffer solution to completely dissolve the precipitate, add 4% neutral protease, and incubate at 50°C Decompose for 6.5 hours, inactivate the enzyme at 70°C for 5 minutes, add citric acid to adjust the pH to 5.6, add 8% compound enzyme, hydrolyze for 6 hours at 47°C, inactivate the enzyme for 10 minutes at 85°C, filter with ultrafiltration membrane, and the separation molecular weight is 500Da-5000Da The apricot peptides for use.
Embodiment 3
[0045] Take 500g of apricot, wash it, beat it into a slurry, add 0.01mol / L sodium chloride solution 15 times the amount of apricot, ultrasonically extract at 45°C for 25min, centrifuge at 4500r / min to get the supernatant, add citric acid to adjust the pH Centrifuge at 3.5, 3000r / min to get the precipitate, wash with water, keep warm at 85°C for 10min, centrifuge at 3500r / min to get the precipitate, add an appropriate amount of neutral phosphate buffer solution to completely dissolve the precipitate, add 5% neutral protease, and incubate at 45°C Decompose for 5 hours, inactivate the enzyme at 70°C for 5 minutes, add citric acid to adjust the pH to 4.6, add 4% compound enzyme, enzymatically hydrolyze for 8 hours at 43°C, inactivate the enzyme for 10 minutes at 85°C, use ultrafiltration membrane to filter, and separate the molecular weight of 500Da-5000Da Apricot peptides for use.
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