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A method for oxidizing chitosan quaternary ammonium salt cross-linked collagen

A technology of oxidizing chitosan and quaternary ammonium salt, applied in bandages, surgery, surgical adhesives, etc., can solve the problems of difficult to meet the requirements of use, large steric hindrance, low degree of cross-linking, etc., to overcome the adverse effects , low cross-linking conditions, reducing the effect of antigenicity

Active Publication Date: 2018-11-27
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Chitosan quaternary ammonium salt has good biocompatibility and excellent antibacterial properties, but its interaction with collagen is weak, and it is difficult to directly form a strong covalent bond
Simple physical blending can improve the use performance of collagen to a certain extent, endow antibacterial and antibacterial properties, but it is often difficult to meet the use requirements
Using carbodiimide as a reaction medium, chitosan quaternary ammonium salt can be covalently linked to collagen, but due to the steric hindrance of the two molecules, the degree of crosslinking is low, and by-products may occur , can not better meet the needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Dissolve type I collagen in acetic acid-sodium acetate solution (0.5M, pH4) at 4°C to prepare a 5 mg / mL type I collagen solution;

[0028] (2) Prepare oxidized O-hydroxypropyltrimethylammonium chloride N-trimethylchitosan with a degree of oxidation of 75% into a solution with a concentration of 0.4mg / mL, and gradually dissolve it at 4°C Add dropwise to 5 mg / mL type I collagen solution, so that the final mass concentration is 5% of the collagen concentration;

[0029] (3) React with magnetic stirring at 4°C for 24 hours;

[0030] (4) Add lysine solution with a concentration of 10mg / mL to the solution so that the final mass concentration is 1% of the collagen concentration, and react with magnetic stirring at 4°C for 6h;

[0031] (5) Inject the collagen solution into a dialysis bag with a molecular weight cut-off of 3500Da, dialyze in water for 3 days, and change the water for injection every 8 hours;

[0032] (6) Freeze-dry the cross-linked collagen using a freeze ...

Embodiment 2

[0034] (1) Weigh 3 grams of collagen and dissolve it in 3000 ml of acetic acid solution with pH 5.0 at 4°C;

[0035] (2) Weigh 0.5 g of oxidized N,N,N-trimethyl chitosan quaternary ammonium salt with an oxidation degree of 40%, place it in 50ml of acetic acid solution with pH 5.0, and shake it to dissolve completely;

[0036] (3) Slowly add the oxidized chitosan quaternary ammonium salt solution dropwise to the collagen solution, and keep stirring for 12 hours at 4°C;

[0037] (4) Add 0.06 g of glycine to the collagen solution, and keep stirring for 8 hours at 4°C;

[0038] (5) Inject the reaction solution into a dialysis bag with a molecular weight cut-off of 5000 Da, dialyze in ultrapure water for 5 days, and change the ultrapure water every 12 hours;

[0039] (6) Air-dry the dialyzed collagen solution to form a collagen film.

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PUM

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Abstract

The invention discloses an oxidized chitosan quaternary ammonium salt and collagen crosslinking method. The method comprises the steps that oxidized chitosan quaternary ammonium salt containing active formyl groups is crosslinked with collagen under certain conditions, and chitosan quaternary ammonium salt is introduced into collagen in the mode of covalent bonds. According to the method, by means of collagen crosslinking, collagen can achieve double bacteria resistance and bacteriostasis performance on the basis of improving the use performance of collagen and keeping the biocompatibility of collagen, and a new way is created for new-generation collagen materials with good bacterium resistance and biocompatibility. The method can be used for preparing biological materials.

Description

technical field [0001] The invention relates to a method for oxidizing chitosan quaternary ammonium salt to crosslink collagen, belonging to the field of biomedical materials. Background technique [0002] Collagen is the main component of the extracellular matrix. It is the most abundant and widely distributed protein in animals. It is rich in animal skin, bones, tendons, visceral interstitial cells, ligaments, blood vessels, and sclera. Collagen has many advantages such as low immunogenicity, low irritation, low cytotoxicity, good biocompatibility, good performance of promoting cell growth and biodegradability, and is widely used in the field of biomedicine. But at the same time, collagen also has disadvantages such as poor physical and mechanical properties, intolerance to degradation, and susceptibility to microorganisms, which often require cross-linking and functionalization to improve. [0003] Infection by pathogenic microorganisms such as bacteria and fungi often c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/24C08J7/14C08J9/28C08J5/18A61L24/00A61L24/10A61L24/08A61L15/42A61L15/46A61L15/32A61L15/28A61L15/62C08L89/00C08L5/08
CPCA61L15/28A61L15/325A61L15/425A61L15/46A61L15/62A61L24/001A61L24/0015A61L24/0036A61L24/08A61L24/102A61L2300/232A61L2300/404C08J3/246C08J5/18C08J7/14C08J9/28C08J2201/0484C08J2201/0504C08J2389/00C08J2405/08C08L5/08
Inventor 但卫华陈一宁但年华
Owner SICHUAN UNIV
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