Application of astaxanthin and/or lithium chloride in the preparation of drugs for preventing and treating cognitive impairment caused by chronic organophosphate poisoning

A technology for cognitive impairment and lithium chloride, applied in the direction of active ingredients of alkali/alkaline earth metal chlorides, drug combinations, organic active ingredients, etc., can solve problems that endanger human health, achieve inhibition of apoptosis, relieve anxiety and depression, Improving the effect of cognitive impairment

Active Publication Date: 2019-07-30
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2010, the detection rate of OPS pesticide residues in Kaifeng City, Henan Province was as high as 14.5%. This long-term low-dose exposure to organophosphorus pesticides seriously endangers human health
Some scholars have also reported that the application of lithium chloride in anti-myocardial apoptosis drugs induced by excessive lipid accumulation (CN201510980704.4), the application of methamphetamine cognitive impairment drugs (CN201610368414.9) and other mental stabilizer treatments However, there is no research and application report on astaxanthin combined with lithium chloride in the preparation of drugs for the prevention and treatment of cognitive impairment caused by chronic organophosphate poisoning

Method used

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  • Application of astaxanthin and/or lithium chloride in the preparation of drugs for preventing and treating cognitive impairment caused by chronic organophosphate poisoning
  • Application of astaxanthin and/or lithium chloride in the preparation of drugs for preventing and treating cognitive impairment caused by chronic organophosphate poisoning
  • Application of astaxanthin and/or lithium chloride in the preparation of drugs for preventing and treating cognitive impairment caused by chronic organophosphate poisoning

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Effects of Astaxanthin Combined with Lithium Chloride Treatment on the Behavior of Chronic Organophosphate Poisoning Cognitive Impairment Mice

[0028] 1) Morris water maze test to evaluate the effect of astaxanthin combined with lithium chloride on the behavior of poisoned mice

[0029] The animal model of chronic organophosphate poisoning was established by subcutaneous injection in mice. After the exposure, the Morris water maze was used to test the memory reproduction and spatial memory ability of the mice. For a total of 6 days, take the average escape latency of the mice in the control group within 5 days as the reference value, and calculate the ratio of the difference between the average escape latency of the model mice for 5 days and the reference value to the average escape latency of the mouse, and the value > 20% consider the model successful, and the model success rate is 90%.

[0030] After the model was successfully prepared, the astaxanthin ...

Embodiment 2

[0052] Example 2. Effect of Astaxanthin Combined with Lithium Chloride Treatment on Histomorphology of Hippocampus in Chronic Organophosphate Poisoning Cognitive Impairment Mice

[0053] 1) Congo red staining to evaluate the effect of astaxanthin combined with lithium chloride on the hippocampal injury of poisoned mice

[0054] The animal model of chronic organophosphate poisoning was established by subcutaneous injection in mice. After 8 weeks of exposure and treatment with various drugs, the skulls were opened to take hippocampal tissue for paraffin sectioning, and Congo red staining was used to evaluate the effect of astaxanthin combined with lithium chloride treatment on hippocampal injury in poisoned mice.

[0055] The result of Congo red staining figure 1 As shown, no orange-red amyloid deposits were found in the hippocampal slices of mice in the control group, and the dorsal cell bands of the hippocampal dentate gyrus were arranged neatly; Obvious striped orange-red d...

Embodiment 3

[0060] Example 3. The effect of astaxanthin combined with lithium chloride treatment on the activation level of Akt / GSK3β / CREB signaling pathway in mice with cognitive impairment in chronic organophosphate poisoning

[0061] 1) Immunohistochemical staining was used to evaluate the effect of astaxanthin combined with lithium chloride on the activation level of Akt / GSK3β / CREB signaling pathway in poisoned mice

[0062] The animal model of chronic organophosphate poisoning was established by subcutaneous injection in mice. After 8 weeks of exposure and treatment with various drugs, the skull was opened to take hippocampal tissue for paraffin sectioning, and immunohistochemical staining was used to evaluate the effect of astaxanthin combined with lithium chloride on the Akt / GSK3β / CREB signal in the exposed mice. Effects on pathway activation levels.

[0063] The results of immunohistochemical staining were as Figure 3-Figure 5 as shown, image 3 The results of p-PI3K immunohis...

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Abstract

The invention provides application of astaxanthin and / or lithium chloride in preparation of drugs for preventing and treating cognitive disorder diseases caused by chronic organophosphorus poisoning. Astaxanthin and lithium chloride inhibitors are used in a combined manner, the treatment effect of a group which uses the astaxanthin and lithium chloride inhibitors in the combined manner is more obvious than the treatment effect of a group which independently uses astaxanthin, and phosphorylation level of protein of Akt / GSK3 beta / CREB signal channels in brains of mice is remarkably higher than that of a model group. Therefore, a treating mode of using the astaxanthin and the lithium chloride in a combined manner has remarkable neuroprotective effect of resisting oxidation and inhibiting apoptosis, and the cognitive function of OPS poisoned mice is improved. The treating mode of using the astaxanthin and the lithium chloride in the combined manner has the neuroprotective effect on hippocampus injury caused by chronic organophosphorus poisoning, cognitive function disorder caused by chronic organophosphorus poisoning is improved by activating the Akt / GSK3 beta / CREB signal channels, and therefore, the treating reagent is expected to be developed into novel drugs for preventing and treating cognitive disorder caused by chronic organophosphorus poisoning.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to the application of astaxanthin and / or lithium chloride in the preparation of medicines for preventing and treating cognitive impairment caused by chronic organophosphate poisoning. Background technique [0002] Organophosphate pesticides (organophosphate pesticides, OPS) are currently widely used insecticides in the world. They have the characteristics of broad insecticidal spectrum, high efficacy and low price, and are widely used in household and agricultural pest control. In my country, according to the survey data of the State Administration of Quality Management, there are a large number of pesticide residues in food in our country, and the exceeding standard rate of organophosphorus pesticides in crops is 1%-35%. In 2010, the detection rate of OPS pesticide residues in Kaifeng City, Henan Province was as high as 14.5%. This long-term low-dose exposure to organophosphoru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/14A61K31/122A61P25/28A61P25/22A61P25/24
CPCA61K31/122A61K33/14A61K2300/00
Inventor 郝翠张美增郭云良乔静文王伟张丽娟
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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